Effect of cold plasma treatment on the antigenicity of peanut allergen Ara h 1

Venkataratnam, Harshitha; Sarangapani, Chaitanya; Cahill, Orla; Ryan, Catherine Barry

doi:10.1016/j.ifset.2019.02.001

Cited by 78 publications

(54 citation statements)

References 50 publications

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“…The changes in the percentage of conformational units for both Ara h 1 and Ara h 2 are presented in Table 1. Thus, implying that both Ara h 1 and Ara h 2 predominantly contain α-helix and these results are consistence with previous results from Venkatratnam et al 23 . With increases in the plasma treatment dose, a progressive decrease in the molar ellipticity was observed for both Ara h 1 and Ara h 2 in DPF and WP.…”

Section: Sds-pagesupporting

confidence: 93%

“…Additionally, the difference in the reduction in antigenicity of these allergens are due to changes in the protein structure. Similar reductions were achieved by Venkataratnam et al 23 for cold plasma treatment of peanut allergens. Wu et al 43 reported that the mechanism of plasma allergen reduction depends on the form of protein structure.…”

Section: Sds-pagesupporting

confidence: 74%

“…The decrease in the band intensities of WP and DPF could be attributed to plasma induced changes in the protein structure or oxidation of protein resulting in the formation of disulphide bonds. An earlier study using an atmospheric DBD plasma system, resulted in no changes in Ara h 1 for either WP and DPF 23 . Many authors have reported that the effects induced by plasma depends on the system configuration 21,37 .…”

Section: Sds-pagementioning

confidence: 77%

“…SDS-PAGE gels were prepared, as described in Sect. 2.4 and the proteins separated were transferred to nitrocellulose membrane at a constant voltage of 20 V for 1 h. Immunoblotting was performed according to Venkataratnam et al 23 with slight modifications. The nitrocellulose membrane were then blocked with 5% skim milk in phosphate-buffer saline containing 2% Tween 20 (PBST) for 2 h at room temperature, followed by overnight incubation with rabbit anti-Ara h 1 (1:5,000 in PBST, Indoor Biotechnologies) and rabbit anti-Ara h 2 (1:5,000 in PBST, Indoor Biotechnologies) at 4 °C.…”

Section: Extraction and Purificationmentioning

confidence: 99%

“…Several authors have reported possible changes in the protein structure of the PPO and POD enzymes due to plasma treatment 22 . Recently, Meinlschmidt et al 20 and Venkataratnam et al 23 reported changes in the immunoreactivity of soy and allergenicity of peanut after cold plasma treatment. However, data on the effect of cold plasma on the allergenicity of major peanut allergens are sparse.…”

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confidence: 99%

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Impact of cold plasma processing on major peanut allergens

Venkataratnam

Cahill

Sarangapani

et al. 2020

Sci Rep

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Cold plasma is emerging as a novel food processing technology, with demonstrated efficacies for microbial inactivation and residual chemical dissipation of food products. Given the technology’s multimodal action it has the potential to reduce allergens in foods, however data on the efficacy and mechanisms of action are sparse. This study investigates the efficacy of cold plasma on major peanut allergens (Ara h 1 and Ara h 2). For this purpose, dry, whole peanut (WP) and defatted peanut flour (DPF) were subjected to an atmospheric air discharge using a pin to plate cold plasma reactor for different treatment durations. With increases in plasma exposure, SDS-PAGE analysis revealed reduced protein solubility of the major peanut allergens. Alterations in allergenicity and structure of Ara h 1 and Ara h 2 were examined using ELISA and circular dichroism (CD) spectroscopy. Competitive ELISA with proteins purified from plasma treated WP or DPF revealed reduced antigenicity for both Ara h 1 and Ara h 2. The highest reduction in antigenicity was 65% for Ara h 1 and 66% Ara h 2 when purified from DPF. Results from CD spectroscopy analysis of purified proteins strongly suggests the reduction in antigenicity is due to modifications in the secondary structure of the allergens induced by plasma reactive species. Cold plasma is effective at reducing peanut protein solubility and causes changes in allergen structure leading to reduced antigenicity.

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Section: Sds-pagesupporting

confidence: 93%

Section: Sds-pagesupporting

confidence: 74%

Section: Sds-pagementioning

confidence: 77%

Section: Extraction and Purificationmentioning

confidence: 99%

mentioning

confidence: 99%

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Impact of cold plasma processing on major peanut allergens

Venkataratnam

Cahill

Sarangapani

et al. 2020

Sci Rep

Self Cite

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Mitigating the allergenicity of peanut allergen Ara h 1 by cold atmospheric pressure argon plasma jet

et al. 2023

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BACKGROUND Peanut allergy is recognized as a major food allergy that triggers severe and even fatal symptoms. Avoidance of peanuts in the diet is the main option for current safety management. Processing techniques reducing peanut allergenicity are required to develop other options. Cold plasma is currently considered as a novel non‐thermal approach to alter protein structure and has the potential to alleviate immunoreactivity of protein allergen. RESULTS The application of a cold argon plasma jet to peanut protein extract could reduce the amount of a 64 kDa protein band corresponding to a major peanut allergen Ara h 1 using sodium dodecyl sulfate–polyacrylamide gel electrophoresis, but the overall protein size distribution did not change significantly. A decrease in peanut protein solubility was a possible cause that led to the loss of protein content in the soluble fraction. Immunoblotting and enzyme‐linked immunosorbent assay elucidated that the immunoreactivity of Ara h 1 was significantly decreased with the time treated with plasma. Ara h 1 antigenicity reduced by 38% after five scans (approximately 3 min) of cold argon plasma jet treatment, and the reduction was up to 66% after approximately 15 min of treatment. CONCLUSION The results indicate that cold argon plasma jet treatment could be a suitable platform for alleviating the immunoreactivity of peanut protein. This work demonstrates an efficient, compact, and rapid platform for mitigating the allergenicity of peanuts, and shows great potential for the plasma platform as a non‐thermal technique in the food industry. © 2023 Society of Chemical Industry.

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Current Technology Readiness Levels (TRL) of Nonthermal Technologies and Research Gaps for Improved Process Control and Integration into Existing Production Lines

Ebert

Aganovic

2022

Food Engineering Series

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Effect of cold plasma treatment on the antigenicity of peanut allergen Ara h 1

Cited by 78 publications

References 50 publications

Impact of cold plasma processing on major peanut allergens

Impact of cold plasma processing on major peanut allergens

Mitigating the allergenicity of peanut allergen Ara h 1 by cold atmospheric pressure argon plasma jet

Current Technology Readiness Levels (TRL) of Nonthermal Technologies and Research Gaps for Improved Process Control and Integration into Existing Production Lines

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