Effect of Clostridium butyricum on High-Fat Diet-Induced Intestinal Inflammation and Production of Short-Chain Fatty Acids

Choi, Yonghoon; Choi, Soo In; Kim, Na Young; Nam, Ryoung Hee; Jang, Jae Young; Na, Hee Young; Shin, Cheol Min; Lee, Dong Ho; Min, Huitae; Kim, Yeon-Ran; Seok, Yeong‐Jae

doi:10.1007/s10620-023-07835-2

Cited by 13 publications

(12 citation statements)

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“… 36 In contrast, the effect of C. butyricum on HFD-induced intestinal inflammation was more prominent in males than in females. 15 In the present study, the effect of Biovita and C. butyricum was different depending on sex. The Firmicutes/Bacteroidetes ratio, which is known to increase with obesity and a HFD, was not evident in our present study.…”

Section: Discussionmentioning

confidence: 44%

“…To assess the influence of gut microbial changes on colonic epithelial cell status, we determined mRNA expression in the colonic mucosa in a previous study. 15 The results of mRNA expression levels in colonic mucosa were compared with those of the relative abundance of A. muciniphila , which could be the key microbial member influence to colonic mucosal environment comprising stool microbiota with an average of 20% in all groups ( Fig. 3 ).…”

Section: Resultsmentioning

confidence: 99%

“…This effect was supported by the increased concentration of fecal butyric acid by administration of C. butyricum groups. 15 …”

Section: Discussionmentioning

confidence: 99%

“…The diet and administration of probiotics in each group has been described in detail in a previous paper and are shown in Supplementary Figure 1 . 15 As there is an abbreviation for each group, the name and number of rats in each group are as follows ( Supplementary Fig. 1A ): males with a control diet (M. CON, n = 8); female rats with a chow diet (F. CON, n = 8); males with a HFD (M. HFD, n = 8); females with a HFD (F. HFD, n = 8); males with a HFD and Biovita (M. Bio, n = 8); females with the HFD and Biovita (F. Bio, n = 8); males with a HFD and low concentrations of C. butyricum (M. LCB, n = 8); females with a HFD and low concentrations of C. butyricum (F. LCB, n = 8); male rats with a HFD and medium concentrations of C. butyricum (M. MCB, n = 8); female rats with a HFD and medium concentrations of C. butyricum (F. MCB, n = 8); male with a HFD and high concentrations of C. butyricum (M. HCB, n = 8); and females with a HFD and high concentrations of C. butyricum (F. HCB, n = 8).…”

Section: Methodsmentioning

confidence: 99%

“… 13 , 14 A previous study found an improvement in high-fat diet (HFD)-induced colonic inflammation by Clostridium butyricum and Biovita through the production of butyric acid in young F344 rats, and the changes were found to be more distinguished in males than in females. 15 The commercialized probiotic complex with 3 bacterial species, anaerobic C. butyricum , facultative anaerobe Lactobacillus sporogenes , and aerobic Bacillus subtilis, Biovita (Ildong Pharmaceutical, Co, Ltd, Seoul, Korea) has been widely used to treat constipation, loose stool, abdominal bloating, and functional gastrointestinal disorders from their various proliferative sites depends on oxygen concentration of gastrointestinal tract. 16 Moreover, all 3 bacteria could form spore which keeps probiotics easy to store and stable delivery to the intestine.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Clostridium butyricum on Gut Microbial Changes and Functional Profiles of Metabolism in High-fat Diet–fed Rats Depending on Age and Sex

Choi,

Kim,

Choi

et al. 2024

J Neurogastroenterol Motil

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Background/Aims A high-fat diet (HFD) causes dysbiosis and promotes inflammatory responses in the colon. This study aims to evaluate the effects of Clostridium butyricum on HFD-induced gut microbial changes in rats. Methods Six-week-old Fischer-344 rats with both sexes were given a control or HFD during 8 weeks, and 1-to-100-fold diluted Clostridium butyricum were administered by gavage. Fecal microbiota analyses were conducted using 16S ribosomal RNA metagenomic sequencing and predictive functional profiling of microbial communities in metabolism. Results A significant increase in Ruminococcaceae and Lachnospiraceae , which are butyric acid-producing bacterial families, was observed in the probiotics groups depending on sex. In contrast, Akkermansia muciniphila , which increased through a HFD regardless of sex, and decreased in the probiotics groups. A. muciniphila positively correlated with Claudin-1 expression in males ( P < 0.001) and negatively correlated with the expression of Claudin-2 ( P = 0.042), IL-1β ( P = 0.037), and IL-6 ( P = 0.044) in females. In terms of functional analyses, a HFD decreased the relative abundances of M00131 (carbohydrate metabolism module), M00579, and M00608 (energy metabolism), and increased those of M00307 (carbohydrate metabolism), regardless of sex. However, these changes recovered especially in male C. butyricum groups. Furthermore, M00131, M00579, and M00608 showed a positive correlation and M00307 showed a negative correlation with the relative abundance of A. muciniphila ( P < 0.001). Conclusion The beneficial effects of C. butyricum on HFD-induced gut dysbiosis in young male rats originate from the functional profiles of carbohydrate and energy metabolism.

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Section: Discussionmentioning

confidence: 44%

Section: Resultsmentioning

confidence: 99%

“…This effect was supported by the increased concentration of fecal butyric acid by administration of C. butyricum groups. 15 …”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%