Effect of cholesterol on the hydration properties of ester and ether lipid membrane interphases

Pérez, Hugo Alejandro; Alarcón, L. M.; Verde, Alejandro R.; Appignanesi, Gustavo A.; Giménez, Rodrigo Esteban; Disalvo, E.A.; Frías, María de los Ángeles

doi:10.1016/j.bbamem.2020.183489

Cited by 9 publications

(6 citation statements)

References 38 publications

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“…The fusogenic effect of cholesterol could be two-fold: on the one hand, cholesterol may increase the lipid packing stress, leading to the formation of membrane defects which promote fusion, while on the other hand it induces dehydration of the membrane interface, thus facilitating the approach between the fusogenic bilayers. [57][58][59] Consistently, further increasing cholesterol content up to 50% mol caused a further increase in fusion efficiency, as displayed in Fig. S4c,f.…”

Section: Increased Dope and Cholesterol Concentration Improves Lipid ...supporting

confidence: 73%

Modulating membrane fusion through the design of fusogenic DNA circuits and bilayer composition

et al. 2022

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Section: Increased Dope and Cholesterol Concentration Improves Lipid ...supporting

confidence: 73%

Modulating membrane fusion through the design of fusogenic DNA circuits and bilayer composition

et al. 2022

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“…In membrane composed of ester-linked phospholipids, laurdan GP values of approximately 0.5 denote ordered gel phases, whereas values below zero are typical for fluid-like lipid phases. Due to the absence of a carboxyl group in ether bound lipids, such as those used in the current work, the position of laurdan in the membrane is somewhat different, and the impact on laurdan GP is still debated 58 . The insertion of laurdan may also be impacted by the presence of the polyisoprenoid chains instead of acyl chains.…”

Section: Resultsmentioning

confidence: 99%

Characterisation of a synthetic Archeal membrane reveals a possible new adaptation route to extreme conditions

et al. 2021

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It has been proposed that adaptation to high temperature involved the synthesis of monolayer-forming ether phospholipids. Recently, a novel membrane architecture was proposed to explain the membrane stability in polyextremophiles unable to synthesize such lipids, in which apolar polyisoprenoids populate the bilayer midplane and modify its physico-chemistry, extending its stability domain. Here, we have studied the effect of the apolar polyisoprenoid squalane on a model membrane analogue using neutron diffraction, SAXS and fluorescence spectroscopy. We show that squalane resides inside the bilayer midplane, extends its stability domain, reduces its permeability to protons but increases that of water, and induces a negative curvature in the membrane, allowing the transition to novel non-lamellar phases. This membrane architecture can be transposed to early membranes and could help explain their emergence and temperature tolerance if life originated near hydrothermal vents. Transposed to the archaeal bilayer, this membrane architecture could explain the tolerance to high temperature in hyperthermophiles which grow at temperatures over 100 °C while having a membrane bilayer. The induction of a negative curvature to the membrane could also facilitate crucial cell functions that require high bending membranes.

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“…As seen in Table 1, the measured anisotropy values in the Chol-containing membranes are comparable with those obtained in pure PC and PC/CL membranes, therefore the TDV location in the non-polar membrane part seems less probable due to a well-known ability of Chol to produce tighter lateral packing of lipid molecules (condensing effect) [26]. An assumption was made that the observed increase in p K value in the presence of cholesterol is associated with the ability of Chol to alter hydration and packing density of lipid membranes [27][28][29][30]. It was previously shown that cholesterol is capable of producing the increase in separation of phospholipid headgroups [27].…”

Section: Resultsmentioning

confidence: 95%

“…It was previously shown that cholesterol is capable of producing the increase in separation of phospholipid headgroups [27]. Moreover, numerous studies provide evidence for the decreased polarity at the level of glycerol backbone of phospholipids in the presence of cholesterol [28][29][30]. The TDV sensitivity to the membrane hydration is confirmed by the observed blue shift of the position of emission maxima in the presence of Chol in comparison with CL-containing and pure PC membranes, indicating that TDV accommodates in the less polar environment in the presence of cholesterol.…”

Section: Resultsmentioning

confidence: 98%

Interactions of Novel Phosphonium Dye with Lipid Bilayers: A Fluorescence Study

Zhytniakivska

2021

EEJP

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The phosphonium-based optical probes attract ever growing interest due to their excellent chemical and photophysical stability, high aqueous solubility, long wavelength absorption and emission, large extinction coefficient, high fluorescence quantum yield, low cytotoxicity, etc. The present study was focused on assessing the ability of the novel phosphonium dye TDV to monitor the changes in physicochemical properties of the model lipid membranes. To this end, the fluorescence spectral properties of TDV have been explored in lipid bilayers composed of zwitterionic lipid phosphatidylcholine (PC) and its mixtures with cholesterol (Chol) or/and anionic phospholipid cardiolipin (CL). It was observed that in the buffer solution TDV possesses one well-defined fluorescence peak with the emission maximum at 533 nm. The dye transfer from the aqueous to lipid phase was followed by the enhancement of the fluorescence intensity coupled with a red shift of the emission maximum up to 67 nm, depending on the liposome composition. The quantitative information about the dye partitioning into lipid phase of the model membranes was obtained through approximating the experimental dependencies of the fluorescence intensity increase vs lipid concentration by the partition model. Analysis of the partition coefficients showed that TDV has a rather high lipid-associating ability and displays sensitivity to the changes in physicochemical properties of the model lipid membranes. The addition of CL, Chol or both lipids to the PC bilayer gives rise to the increase of the TDV partition coefficients compared to the neat PC membranes. The enhancement of the phosphonium dye partitioning in the CL and Chol-containing lipid bilayers has been attributed to the cardiolopin- and cholesterol-induced changes in the structure and physicochemical characteristics of the polar membrane region.

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Effect of cholesterol on the hydration properties of ester and ether lipid membrane interphases

Cited by 9 publications

References 38 publications

Modulating membrane fusion through the design of fusogenic DNA circuits and bilayer composition

Modulating membrane fusion through the design of fusogenic DNA circuits and bilayer composition

Characterisation of a synthetic Archeal membrane reveals a possible new adaptation route to extreme conditions

Interactions of Novel Phosphonium Dye with Lipid Bilayers: A Fluorescence Study

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