Effect of chloride on p-aminohippurate analyses by the Bratton-Marshall method.

Waugh, William H.

doi:10.1152/jappl.1974.37.5.752

Cited by 5 publications

(3 citation statements)

References 6 publications

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“…Smith et al (1945) modified this procedure by producing a cadmium sulfate filtrate of plasma or urine that was first acidified by 0.2 volume of 1.2N hydrochloric acid prior to the sequential steps noted above. It was several decades later that it was realized that chloride and temperature were critical for the colour development in these assays (Waugh, 1974). Nevertheless, fresh preparation of reagents (nitrite and sulfamate), sequential addition of three reagents, timing of which is critical, and temperature dependence of these assays make these techniques cumbersome.…”

Section: Discussionmentioning

confidence: 99%

A simple technique for the detection of anti-malarial drug formulations and their presence in human urine

Lugimbana¹,

Malebo²,

Segeja³

et al. 2009

Tanzania J Hlth Res

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A simple, sensitive, specific assay technique for the detection and semi-quantification of chloroquine, amodiaquine, quinine, primaquine, sulfadoxine and pyrimethamine in formulations and in human urine by using thin layer chromatography (TLC) was developed and tested in the laboratory. The method involved developing test samples spotted on TLC chromatogram by diethylamine-toluene-isopropanol (1:4:5 v/v/v) as the eluting solvent. The solvent system diethylamine-toluene-isopropanol (1:4:5 v/v/v) enabled the elution and detection of all the tested antimalarial drugs in solution and those spiked in human urine. Detection limits for chloroquine, amodiaquine, quinine and primaquine were the lowest at 0.00025 mg/ml. Sulfadoxine exhibited a detection limit of 0.0005 mg/ml whereas that of pyrimethamine was 0.001 mg/ml. The results indicate the suitability of this technique in antimalarial drug quality and bioavailability studies. It is envisaged that this technique will adequately address the role of drug absorption and excretion in the chemotherapy of malaria as well as to detect types of antimalarial drugs commonly used in the community.

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Section: Discussionmentioning

confidence: 99%

A simple technique for the detection of anti-malarial drug formulations and their presence in human urine

Lugimbana¹,

Malebo²,

Segeja³

et al. 2009

Tanzania J Hlth Res

View full text Add to dashboard Cite

show abstract

“…Smith et al (19) modified this procedure by producing a cadmium sulfate filtrate of plasma or urine that was first acidified by 0.2 volume of 1.2 N-hydrochloric acid before the sequential steps noted above. It was several decades later that it was realized that chloride and temperature were critical for color development in the above assays (22). The above assay is not specific for p-aminohippuric acid.…”

Section: Discussionmentioning

confidence: 99%

Rapid microplate method for PAH estimation

Agarwal

2002

American Journal of Physiology-Renal Physiology

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Evaluation of renal hemodynamics requires estimation of effective renal plasma flow, which is commonly measured by the renal clearance of p-aminohippuric acid (PAH). There are many existing methods for PAH assay that are complicated, expensive, or time consuming. We describe a rapid, precise, and accurate microplate-based assay of PAH using p-dimethylaminocinnamaldehyde, which produces a red color on reaction with PAH, and compare it with a reference HPLC method. Renal PAH clearances were measured in 10 volunteers, and clearances were calculated by using the new and HPLC methods. There was excellent agreement between the HPLC and the microplate method of PAH assay. The average ratio of microplate to HPLC method was nearly 1.0, and the limits of agreement (2 SD) for plasma, urine, and clearances were 17.2, 19.3, and 25.5%, respectively. Intraday coefficient of variation for urine and plasma was <7%; interday coefficient of variation was <6% for urine and plasma samples. The microplate method is a reliable alternative to a reference HPLC method and can be performed for a fraction of the cost, time, and reagents.

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“…94 In the dedication of his History of Ireland to the Earl of Leicester ( 1571) Campion recalled 'how often at Oxford, how often at the Court, how at Rycote, how at Windsor ... you have not ceased to further with advice and countenance with authority the hope and expectation of me, a single student'. 95 On 29 June 1580, a fortnight after landing in England, he preached in the hall of Lord Norreys's house in London. 96 So perhaps he also returned, in disguise, to Rycote.…”

Section: Campion In the Thames Valley 1580mentioning

confidence: 99%

Campion in the Thames Valley, 1580

Hodgetts¹

2010

Recusant History

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Between July and October 1580, Robert Persons and Edmund Campion, who had landed at Dover in June, ‘passed through the most part of the shires of England, preaching and administering the sacraments in almost every gentleman's and nobleman's house that we passed by, whether he was Catholic or not, provided he had any Catholics in his house to hear us’. Between Christmas 1580 and Whitsun 1581 Campion went on a similar journey to the North, the itinerary of which can be recovered in fair detail from entries in the Acts of the Privy Council and from a summary of the subsequent examinations which was annotated by Lord Burghley. The mission of Campion and Persons (and of the dozen or so others who accompanied them from Rome) has rightly been regarded as a pivotal event in the story of Elizabethan recusancy. But no serious attempt has ever been made to reconstruct their itineraries in 1580.

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Effect of chloride on p-aminohippurate analyses by the Bratton-Marshall method.

Cited by 5 publications

References 6 publications

A simple technique for the detection of anti-malarial drug formulations and their presence in human urine

A simple technique for the detection of anti-malarial drug formulations and their presence in human urine

Rapid microplate method for PAH estimation

Campion in the Thames Valley, 1580

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