Effect of buried potential barrier in label-less electrochemical immunodetection of glutathione and glutathione-capped gold nanoparticles

Stobiecka, Magdalena; Hepel, Maria

doi:10.1016/j.bios.2011.01.038

Cited by 56 publications

(21 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…1 and 3). The similar phenomenon was reported by Stobiecka and Hepel [23]. They stated that the charge redistribution was responsible for signal transduction in label-free electrochemical glutathione immunodetection.…”

Section: Detection Of the Anti-hemagglutinin Antibodies In Hen Serasupporting

confidence: 55%

A biosensor based on electroactive dipyrromethene-Cu(II) layer deposited onto gold electrodes for the detection of antibodies against avian influenza virus type H5N1 in hen sera

et al. 2015

View full text Add to dashboard Cite

This paper describes the development of a biosensor for the detection of anti-hemagglutinin antibodies against the influenza virus hemagglutinin. The steps of biosensor fabrications are as follows: (i) creation of a mixed layer containing the thiol derivative of dipyrromethene and 4-mercapto-1-butanol, (ii) complexation of Cu(II) ions, (iii) oriented immobilization of the recombinant histidine-tagged hemagglutinin, and (iv) filling free spaces with bovine serum albumin. The interactions between recombinants hemagglutinin from the highly pathogenic avian influenza virus type H5N1 and anti-hemagglutinin H5 monoclonal antibodies were explored with Osteryoung square-wave voltammetry. The biosensor displayed a good detection limit of 2.4 pg/mL, quantification limit of 7.2 pg/mL, and dynamic range from 4.0 to 100.0 pg/mL in buffer. In addition, this analytical device was applied for the detection of antibodies in hen sera from individuals vaccinated and non-vaccinated against the avian influenza virus type H5N1. The limit of detection for the assay was the dilution of sera 1: 7 × 10(6), which is about 200 times better than the enzyme-linked immunosorbent assay.

show abstract

Section: Detection Of the Anti-hemagglutinin Antibodies In Hen Serasupporting

confidence: 55%

A biosensor based on electroactive dipyrromethene-Cu(II) layer deposited onto gold electrodes for the detection of antibodies against avian influenza virus type H5N1 in hen sera

et al. 2015

View full text Add to dashboard Cite

show abstract

“…In general, those publications that report a lower limit of detection require two functionalization protocols, one for the sensing surface and another one for additional nanostructures that act as signal amplifiers. For instance, the methods described by Stobiecka et al (Stobiecka andHepel 2011) andGarcía-Marín et al (García-Marín et al 2014) use gold nanoparticles to enhance the sensitivity after the previous immobilization of the analyte on the NP surface, which can hinder GSH quantification in more complex matrices.…”

Section: Ellipsometric Immunosensor Based On Gallium Nanostructured Smentioning

confidence: 99%

Immunosensing platform based on gallium nanoparticle arrays on silicon substrates

Marín

Hernández

Ruiz

et al. 2015

Biosensors and Bioelectronics

View full text Add to dashboard Cite

show abstract

“…As the consequence, upon binding of the S100B protein, a decrease of the Cu(II) redox current was observed (Figure 3A–C, Table 1). The idea of charge redistribution responsible for signal transduction has been recently reported by Stobiecka and Hepel [36]. …”

Section: Resultsmentioning

confidence: 99%

Voltammetric Detection of S100B Protein Using His-Tagged Receptor Domains for Advanced Glycation End Products (RAGE) Immobilized onto a Gold Electrode Surface

Mikuła

Wysłouch‐Cieszyńska

Zhukova

et al. 2014

Sensors

View full text Add to dashboard Cite

In this work we report on an electrochemical biosensor for the determination of the S100B protein. The His-tagged VC1 domains of Receptors for Advanced Glycation End (RAGE) products used as analytically active molecules were covalently immobilized on a monolayer of a thiol derivative of pentetic acid (DPTA) complex with Cu(II) deposited on a gold electrode surface. The recognition processes between the RAGE VC1 domain and the S100B protein results in changes in the redox activity of the DPTA-Cu(II) centres which were measured by Osteryoung square-wave voltammetry (OSWV). In order to verify whether the observed analytical signal originates from the recognition process between the His6–RAGE VC1 domains and the S100B protein, the electrode modified with the His6–RAGE C2 and His6–RAGE VC1 deleted domains which have no ability to bind S100B peptides were applied. The proposed biosensor was quite sensitive, with a detection limit of 0.52 pM recorded in the buffer solution. The presence of diluted human plasma and 10 nM Aβ1-40 have no influence on the biosensor performance.

show abstract

Effect of buried potential barrier in label-less electrochemical immunodetection of glutathione and glutathione-capped gold nanoparticles

Cited by 56 publications

References 41 publications

A biosensor based on electroactive dipyrromethene-Cu(II) layer deposited onto gold electrodes for the detection of antibodies against avian influenza virus type H5N1 in hen sera

A biosensor based on electroactive dipyrromethene-Cu(II) layer deposited onto gold electrodes for the detection of antibodies against avian influenza virus type H5N1 in hen sera

Immunosensing platform based on gallium nanoparticle arrays on silicon substrates

Voltammetric Detection of S100B Protein Using His-Tagged Receptor Domains for Advanced Glycation End Products (RAGE) Immobilized onto a Gold Electrode Surface

Contact Info

Product

Resources

About