Effect of BDNF secretion by astrocyte on learning and memory: A modeling approach

Hassanpoor, Hossein; Fallah, Ali; Raza, Mohsin

doi:10.1109/icabme.2013.6648850

Cited by 2 publications

(3 citation statements)

References 21 publications

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“…At DIV7, the percentage of glia in the neuronal cultures was estimated by counting GFAP -positive cells and observed to be around 32% in FBS, 23% in B27 and 29% in ACM ( Table 2). As expected, because of the presence of several growth factors 19,21 , FBS and ACM resulted in higher percentages, despite the addition of the proliferation blocker cytosine-β-Darabinofuranoside (Ara-C) at DIV2 (see Methods). The morphological differences were progressively more pronounced with the extension of the culturing time required for glial maturation.…”

Section: Astrocytes Morphology and Percentagementioning

confidence: 79%

“…Finally, since ACM alone is supposed to provide all the nutrients for long -term neuronal survival (e.g. lipids, BDNF and thrombospondins [19][20][21] ), the use of additional growth factors in the culturing medium is not necessary.…”

Section: Discussionmentioning

confidence: 99%

“…Different options are now available for a successful hippocampal cell culture, all of them involving astrocytes: I-direct plating of dissociated neurons on a glial feeder layer 17 , II-suspension of the coverslip with plated neurons above a glial feeder layer 18 and III-culturing neurons in astrocyte-conditioned medium (ACM). This third method has an advantage: it does not require a feeder cell's layer while providing all the soluble factors released by astrocytes, such as growth factors, signaling molecules and lipids [19][20][21] . ACM has been demonstrated to increase neuronal survival and stem cell differentiation [22][23][24] .…”

Section: Introduction *Manuscript (With Page Numbers) Click Here To Dmentioning

confidence: 99%

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An improved method for growing neurons: Comparison with standard protocols

Pozzi

Ban

Iseppon

et al. 2017

Journal of Neuroscience Methods

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Highlights An improved protocol for primary hippocampal cell cultures is proposed.  The method relies on serum-free astrocytes conditioned medium (ACM).  The ACM method is extensively compared with other two commonly used protocols.  ACM improved morphology and function of both short-and long-term cultures. AbstractBackground: Since different culturing parameters -such as media composition or cell density -lead to different experimental results, it is important to define the protocol used for neuronal cultures. The vital role of astrocytes in maintaining homeostasis of neurons -both in vivo and in vitro -is well established: the majority of improved culturing conditions for primary dissociated neuronal cultures rely on astrocytes.New Method: Our culturing protocol is based on a novel serum-free preparation of astrocyteconditioned medium (ACM). We compared the proposed ACM culturing method with other two commonly used methods: Neurobasal/B27-and FBS-based media. We performed morphometric characterization by immunocytochemistry and functional analysis by calcium imaging for all three culture methods at 1,7,14 and 60 days in vitro (DIV).Results: ACM-based cultures gave the best results for all tested criteria, i.e. growth cone's size and shape, neuronal outgrowth and branching, network activity and synchronization, maturation and long-term survival. The differences were more pronounced when compared with FBS-based medium. Neurobasal/B27 cultures were comparable to ACM for young cultures (DIV1), but not for culturing times longer than DIV7. Comparison with Existing Method(s):ACM-based cultures showed more robust neuronal outgrowth at DIV1. At DIV7 and 60, the activity of neuronal network grown in ACM had a more vigorous spontaneous electrical activity and a higher degree of synchronization. Conclusions:We propose our ACM-based culture protocol as an improved and more suitable method for both short-and long-term neuronal cultures.

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Section: Astrocytes Morphology and Percentagementioning

confidence: 79%

Section: Discussionmentioning

confidence: 99%