Effect of Aqueous Extract of Liquorice (G. Glabra L) in Treatment of Stress Induced Gastric Ulcer in Wistar Rats

Abstract

Background: Peptic ulcer is a disease affecting a large number of people, caused due to various reasons including infection with H pylori, consumption of alcohol, NSAIDs and stress. Although not a life-threatening disease, it causes undue distress and discomfort and accounts for increased time off from work thereby leading to manpower and economic losses. In this condition, histamine receptor blockers provide relief. Objectives: Natural remedies for peptic ulcer have not been effectively utilized in clinical set up. In the present study, we evaluated the effect of water extract of Glycyrrhiza Glabra L, commonly known as Liquorice on stressinduced gastric inflammation. Materials & Methods: Liquorice extract was prepared. Male Wistar rats (n=5 in each group) were procured from the central animal house and divided into four groups namely Group 1(control); Group 2 (low dose treatment); Group 3 (high dose treatment) and group 4 (prophylactic). They were housed individually and subjected to restraint stress in PVC pipes for 2 hours daily and their stomach was examined for ulcer in Licorice (oral) fed groups and control. Results: Control group showed the inflammatory changes, ulcer index showed a significant lesion, whereas the animals treated with low dose and high dose showed proportionate decrease in the severity of inflammation as evidenced by the histological features. Fourth group of rats which was treated prophylactically before they were subjected to stress, showed no sign of stress-induced inflammatory changes in the gastric mucosa. Conclusion: The results of this study strongly suggested that the G Glabra extract reduces the inflammation and relieves the ulcers and also it can be used as a prophylactic agent to prevent the peptic ulcer in vulnerable subjects.

A study to compare the effect of milk, licorice and tiger nut on stomach ulcer induced in rats

“…Peptic ulcer is a common ailment which has a prevalence rate of about 0.2% in the population worldwide (Mathur et al, 2019). The symptoms include dysphagia, recurrent vomiting, weight loss, or blood in stools.…”

“…Ant-acids are used in milder cases.Avoidance of oral intake of NSAIDs and aspirin,and other gastric irritants also are useful. However,some traditional treatments were also employed which are less effective (Mathur et al, 2019).From the above it is clear that Peptic ulcer is a disease known since antiquity, but there are few studies innovating diet therapy as treatment for this disease.…”

“…2016) studied the effect of Liquorice and a combination of other herbs namely, Pulasari (Alyxiareinwardtii), and Sembung (Blumeabalsamifera) including a mixture of all these were tested in Gastric ulcer induced with Aspirin in rats.The researchers noted the gastric protective effects of these substances. Recently, Mathur et al,(2019) suggested that the G Glabra extract reduces the inflammation and relieves the ulcers and also it can be used as a prophylactic agent to prevent the peptic ulcer in vulnerable subjects. But Ottenbacher, and blehm (2015)reported that it should be used with caution in patients suffering from cardiovascular disease and/or hypertension.…”

A prospective, single centre, open label, single arm pilot study to evaluate the efficacy and safety of Amlapitta Mishran Suspension in participants with endoscopic gastritis

“…Amlapitta Mishran Suspension is documented for its anti-ulcer effect in an experimental model of indomethacin-induced gastric ulcers in rats [ 5 ]. It has multiple ingredients with different actions that act in synergy and these ingredients possess anti-emetic, anti-ulcer, anti-secretory, gastroprotective, and acid-neutralizing properties [ [6] , [7] , [8] , [9] , [10] , [11] , [12] , [13] , [14] , [15] , [16] , [17] , [18] ].…”

“… Yashti ( Glycyrrhiza glabra ) aqueous extract is reported for its anti-ulcerogenic potential in an experimental model of stress-induced gastric ulcer in Wistar rats [ 14 ]. It is also reported for its healing effect in Helicobacter pylori infected peptic ulcers [ 15 ]. Haritaki ( Terminalia chebula ), Bibhitaka ( Terminalia belerica ), Amalaki ( Emblica officinalis ) in combination are termed as Triphala , these herbs individually and in combination are documented to possess anti-ulcer and cytoprotective effect [ 16 , 17 ].…”