Effect of antioxidants resveratrol and quercetin on in vitro evaluation of frozen ram sperm

Silva, Ellen Cordeiro Bento da; Cajueiro, J.F.P.; Silva, S.V.; Soares, Pierre Castro; Guerra, Maria Madalena Pessoa

doi:10.1016/j.theriogenology.2011.11.023

Cited by 126 publications

(127 citation statements)

References 25 publications

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“…Regarding male reproductive system, some studies have focused in the actions of resveratrol with divergent results (Branco et al, 2010;Collodel et al, 2011;Garcez et al, 2010;Juan et al, 2005;Ourique et al, 2013;Silva et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…In a different specie, ram, the use of RSV in the cryopreservation medium causes a decrease in the mitochondrial membrane potential without any effects in spermatozoa motility, or the integrity of acrosome or plasma membrane (Silva et al, 2012). Collodel et al (2011) describe that 100 µM resveratrol treatment leads to a loss in viability in human spermatozoa as well as in rat spermatocytes, although at 15 µM has a protector effect against ROS.…”

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confidence: 99%

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The Effect of Resveratrol on the Quality of Extended Boar Semen During Storage at 17ºC

Martín‐Hidalgo¹,

Llera²,

Henning³

et al. 2013

JAS

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The natural polyphenol resveratrol may be beneficial to many aspects of cell function and animal health, although its actions in the male reproductive system vary depending on animal species. This work investigates resveratrol effects on the quality of preserved boar semen during liquid storage at 17ºC. We used three approaches: 1) evaluation of conventional parameters of seminal quality, 2) measurement of specific response to capacitating stimuli, and 3) evaluation of mitochondria membrane potential and ATP content. Resveratrol supplementation causes i) a loss in the response of liquid stored boar spermatozoa to capacitating stimuli, ii) a decrease in the sperm ATP content and iii) a reduction in the mitochondrial membrane potential. Moreover, higher concentrations of resveratrol increase plasma membrane phospholipid disorder and reduce the percentage of motile spermatozoa. These results suggest that semen doses supplemented with resveratrol could be considered sub-fertile compared with semen stored hypothermically in standard conditions.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

The Effect of Resveratrol on the Quality of Extended Boar Semen During Storage at 17ºC

Martín‐Hidalgo¹,

Llera²,

Henning³

et al. 2013

JAS

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“…The plasma membrane integrity was determined by a double staining method with carboxyfluorescein diacetate (CFDA; C5041) and propidium iodide (PI; P4170), according to Silva et al (2012). 200 cells per slide were analyzed in an epifluorescence microscope (Carl Zeiss, Göttingen, Germany, 400x), using a DBP 485-520nm excitation filter and a DBP 580-630nm emission filter.…”

Section: Methodsmentioning

confidence: 99%

“…To determine the acrosome integrity, a fluorescein isothiocyanate probe conjugated with peanut agglutinin (FITC-PNA; L7381) was used (Silva et al, 2012). A total number of 200 spermatozoa per slide were examined using a LP 515 nm emission filter and a BP 450-490 nm excitation filter in an epifluorescence microscope (Carl Zeiss, Göttingen, Germany, 1000x).…”

Section: Methodsmentioning

confidence: 99%

“…Both phytochemical agents are found in plants and their derived products (Kelly, 2011;Planas et al, 2011). Despite trans-resveratrol and quercetin being described as potent antioxidants, its effects on semen are inconclusive (Silva et al, 2012). Based on this information and on the need to develop a suitable method to freeze goat semen, the aim of this study was to evaluate the effect of different concentrations of trans-resveratrol or quercetin on the cryopreservation of goat sperm.…”

Section: Introductionmentioning

confidence: 99%

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High resveratrol or quercetin concentrations reduce the oscillation index of frozen goat semen

Silva

Arruda

Silva

et al. 2016

Arq. Bras. Med. Vet. Zootec.

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The aim of this study was to evaluate the effect of different concentrations of trans-resveratrol or quercetin on the ability of goat sperm to withstand being frozen. Six pools of semen obtained from six male goats were processed with different concentrations of resveratrol or quercetin (Experiment 1: 0, 15, 25, 50, 75 or 100µM resveratrol; Experiment 2: 0, 15, 25, 50, 75 or 100µM quercetin) and frozen. After thawing, the semen was evaluated for sperm kinematics, plasma membrane and acrosome integrity, morphology and oxidative stress following 0 and 1h of incubation. Immediately after thawing (0h), wobble (oscillation index) in the groups treated with 100µM of quercetin or resveratrol was lower (P<0.05) than in those treated with 0 and 25µM resveratrol and 0µM quercetin, respectively. After 1h of incubation, the total motility in treatments with 15, 50 and 75µM quercetin, as well as the plasma membrane integrity in all quercetin concentrations were lower (P<0.05) than at 0h. In opposition, the linearity of semen samples treated with 100µM quercetin and the straightness of those treated with 75 and 100µM quercetin were lower (P<0.05) at 0h than at 1h after thawing. Thus, it can be concluded that resveratrol and quercetin at high concentrations (100µM) transiently reduce the wobble of goat sperm submitted to frozen storage, and that quercetin (75 and 100µM) increases the linearity and straightness over time, which can be favorable for fertility.

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Cryopreservation of Sperm: Effects on Chromatin and Strategies to Prevent Them

Paoli¹,

Pelloni²,

Lenzi³

et al. 2019

Genetic Damage in Human Spermatozoa

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Effect of antioxidants resveratrol and quercetin on in vitro evaluation of frozen ram sperm

Cited by 126 publications

References 25 publications

The Effect of Resveratrol on the Quality of Extended Boar Semen During Storage at 17ºC

The Effect of Resveratrol on the Quality of Extended Boar Semen During Storage at 17ºC

High resveratrol or quercetin concentrations reduce the oscillation index of frozen goat semen

Cryopreservation of Sperm: Effects on Chromatin and Strategies to Prevent Them

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