Effect of amino acids on glutathione production by Saccharomyces cerevisiae

Alfafara, Catalino G.; Kanda, Akihisa; Shioi, Toru; Shimizu, Hiroshi; Shioya, Suteaki; Suga, Kazuyoshi

doi:10.1007/bf00170199

Cited by 64 publications

(32 citation statements)

References 11 publications

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“…A high-GSH-accumulating yeast strain, S. cerevisiae KY5711, was used. The components of the culture medium have been described previously (Alfafara et al 1992). Glucose was supplied at 30 g-1-1 for preculture, 10 g.1-1 for batch culture in the jar fermentor and 100-500 g.1-1 for the feed medium.…”

Section: Methodsmentioning

confidence: 99%

“…In Saccharomyces cerevisiae (the commonly used microorganism for commercial GSH production), the effects of cysteine and related compounds on GSH production were checked by Alfafara et al (1992). Cysteine was found to be the only amino acid that enhanced GSH production by increasing the specific GSH production rate twofold compared to the control.…”

Section: Introductionmentioning

confidence: 99%

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Cysteine addition strategy for maximum glutathione production in fed-batch culture of Saccharomyces cerevisiae

Alfafara

Miura

Shimizu

et al. 1992

Appl Microbiol Biotechnol

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A good cysteine addition method that could increase the specific glutathione (GSH) production rate (Pc) was investigated and utilized to maximize total GSH production in fed-batch culture of Saccharomyce s cerevisiae. The single-shot addition of cysteine was a better method compared to a continuous method that maintained a constant cysteine concentration in the.. reactor. The shot method increased Pc about twofold compared to a culture without cysteine. The increase in Pc by the shot method can be achieved without growth inhibition if the cysteine dose is maintained at 0.7 mmol.g-1 cell or less. The positive effect on Pc (at every specific growth rate,/z) was saturated when the cysteine shot concentrations was 3 mM or more. A simple model was developed consisting of mass balance equations and the relationship between p and Pc, for the single cysteine shot addition method. From this model an optimal operating strategy was determined to maximize total GSH production in fed-batch culture. This optimal operation consisted of separating the process into phases of (1) cell growth and (2) GSH production, through a bang-bang profile control of/z, and a shot of cysteine just at the start of the GSH production phase. In other words, the cysteine shot time and the ~ switching time should be the same. For a total feeding time of 10 h, both the switching time of p and cysteine shot time were calculated to be about 6.4 h.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cysteine addition strategy for maximum glutathione production in fed-batch culture of Saccharomyces cerevisiae

Alfafara

Miura

Shimizu

et al. 1992

Appl Microbiol Biotechnol

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“…The free thiol group of cysteine is involved in the formation of disulphide bonds, crucial for the stability of certain proteins, and is also an important catalytic and redox center in various enzymes, cofactors, and regulatory proteins. Cysteine is also the rate-limiting nutrient in glutathione biosynthesis (Alfafara et al 1992;Wen et al 2004), the major redox buffer and detoxification molecule in the cell. Studies have revealed that the entire sulfur assimilation pathway leading to cysteine biosynthesis is upregulated during increased cellular demands of glutathione upon exposure to heavy metals or other toxic compounds in yeasts and plants (Vido et al 2001;Fauchon et al 2002;Aranda and del Olmo 2004;Mendoza-Cozatl et al 2005).…”

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confidence: 99%

Yct1p, a Novel, High-Affinity, Cysteine-Specific Transporter From the Yeast Saccharomyces cerevisiae

Kaur

Bachhawat

2007

Genetics

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Cysteine transport in the yeast Saccharomyces cerevisiae is mediated by at least eight different permeases, none of which are specific for cysteine. We describe a novel, high-affinity, (K m ¼ 55 mm), cysteine-specific transporter encoded by the ORF YLL055w that was initially identified by a combined strategy of data mining, bioinformatics, and genetic analysis. Null mutants of YLL055w, but not of the other genes encoding for transporters that mediate cysteine uptake such as GAP1, GNP1, MUP1, or AGP1 in a met15D background, resulted in a growth defect when cysteine, at low concentrations, was provided as the sole sulfur source. Transport experiments further revealed that Yll055wp was the major contributor to cysteine transport under these conditions. The contributions of the other transporters became relevant only at higher concentrations of cysteine or when YLL055w was either deleted or repressed. YLL055w expression was repressed by organic sulfur sources and was mediated by the Met4p-dependent sulfur regulatory network. The results reveal that YLL055w encodes the principal cysteine transporter in S. cerevisiae, which we have named YCT1 (yeast cysteine transporter). Interestingly, Yct1p belongs to the Dal5p family of transporters rather than the amino acid permease family to which all the known amino acid transporters belong.

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“…However, an increased biomass can inevitably result in lowered intracellular GSH content. Alternatively, addition of precursor amino acids was an easy approach (Alfafala et al, 1992a(Alfafala et al, , 1992b. As it is known, the major functions of plasma membrane were to regulate what comes in and goes out of cells.…”

Section: Disccussionmentioning

confidence: 99%

Novel pH control strategy for glutathione overproduction in batch cultivation of Candida utilis

Liang¹,

Wang²,

Xie³

et al. 2009

Afr. J. Biotechnol.

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The effects of pH values on cell growth and glutathione (GSH) production were studied in batch cultivation of Candida utilis. According to the fact that lower pH value favors cells growth but retards GSH production and higher pH value promotes GSH production while inhibits cells growth, a pH-shift strategy, optimized via simulating Gauss function, was developed. By applying two-stage pH-shift strategy of controlling pH at 5.0 for first 7.5 h and switching to 6.0 afterwards, final GSH yield and productivity reached 279 and 12.7 mg/l/h after 22 h cultivation, increased by 30 and 42%, respectively, compared to constant pH 5.5 operation. Moreover, by feeding glucose instead of sulphuric acid (H 2 SO 4 ) solution to control pH, maximal GSH yield of 315 mg/l was achieved, suggesting application of pH-shift strategy for GSH overproduction as being feasible.

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Effect of amino acids on glutathione production by Saccharomyces cerevisiae

Cited by 64 publications

References 11 publications

Cysteine addition strategy for maximum glutathione production in fed-batch culture of Saccharomyces cerevisiae

Cysteine addition strategy for maximum glutathione production in fed-batch culture of Saccharomyces cerevisiae

Yct1p, a Novel, High-Affinity, Cysteine-Specific Transporter From the Yeast Saccharomyces cerevisiae

Novel pH control strategy for glutathione overproduction in batch cultivation of Candida utilis

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