Effect of Alpinia galanga extract on cartilage degradation and on gene expression in human chondrocyte and synovial fibroblast metabolism

Pothacharoen, Peraphan; Choocheep, Kanyamas; Pitak, Tanyaluck; Pompimon, Wilart; Premanode, Bhusana; Hardingham, Timothy E.; Kongtawelert, Prachya

doi:10.2478/s11535-006-0030-6

Cited by 10 publications

(11 citation statements)

References 32 publications

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“…Cells for pellet culture were trypsinized, and the total number of cells was calculated based on a hemocytometer count: 1 × 10 6 cells/pellet, cultured in 1 ml pellet media (DMEM supplemented with 10% FBS and pen/strep which contained 10 7 M dexamethasone, 25 μg/ml L-ascorbate 2-phosphate and 1× ITS 1+). The growth factor pellet media was basic pellet media plus 100 ng/ml IGF-1 and 10 ng/ml TGFβ3 [ 14 ].…”

Section: Methodsmentioning

confidence: 99%

In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA

et al. 2009

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BackgroundMatrix metalloproteinase (MMPs) synthesized and secreted from connective tissue cells have been thought to participate in degradation of the extracellular matrix. Increased MMPs activities that degrade proteoglycans have been measured in osteoarthritis cartilage. This study aims to suppress the expression of the MMP-3 gene in in vitro human chondrosarcoma using siRNA.MethodsCells were categorized into four groups: control (G.1); transfection solution treated (G.2); negative control siRNA treated (G.3); and MMP-3 siRNA treated (G.4). All four groups were further subdivided into two groups - treated and non-treated with IL-1β- following culture for 48 and 72 h. We observed the effects of gene suppression according to cell morphology, glycosaminoglycan (GAG) and hyaluronan (HA) production, and gene expression by using real-time polymerase chain reaction (PCR).ResultsIn IL-1β treated cells the apoptosis rate in G.4 was found to be lower than in all other groups, while viability and mitotic rate were higher than in all other groups (p < 0.05). The production of GAG and HA in G.4 was significantly higher than the control group (p < 0.05). MMP-3 gene expression was downregulated significantly (p < 0.05).ConclusionMMP-3 specific siRNA can inhibit the expression of MMP-3 in chondrosarcoma. This suggests that MMP-3 siRNA has the potential to be a useful preventive and therapeutic agent for osteoarthritis.

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Section: Methodsmentioning

confidence: 99%

In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA

et al. 2009

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“…Reverse transcription reaction was performed by using 1.0 µg of total RNA and the RNA was reverse-transcribed into cDNA using RevertAid TM First Strand cDNA synthesis kit (Fermentus Inc, Ontario, Canada). The cDNA was amplified for 35 cycles by using two oligonucleotide primers derived from previously published sequences of MMP -1, -2, -3, -13, IL-1ß, ICE, and GAPDH genes (Pothacharoen et al, 2006). The PCR products were subjected to 1.5% agarose gel electrophoresis.…”

Section: Reverse Transcriptase-polymerase Chain Reaction (Rt-pcr)mentioning

confidence: 99%

Active Compound of <em>Zingiber Cassumunar</em> Roxb. Down-Regulates the Expression of Genes Involved in Joint Erosion in a Human Synovial Fibroblast Cell Line

Chaiwongsa

Ongchai²,

Boonsing³

et al. 2012

Afr. J. Trad. Compl. Alt. Med.

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Rheumatoid arthritis (RA) is a chronic inflammatory disease of the synovium. It is involved in up-regulation of pro-inflammatory cytokines and matrix metalloproteinases (MMPs), resulting in joint inflammation and erosion. Zingiber cassumunar Roxb. has long been used to reduce joint pain and inflammation. This study aimed to investigate the inhibitory activities of an active compound of Z. cassumunar, (E)-4-(3',4'-dimethoxyphenyl)but-3-en-1-ol (compound D), against cytokine-induced up-regulation of catabolic genes involved in cartilage degradation in RA. Synovial fibroblast cell line, SW982, was cultured in media containing interleukin-1ß (IL-1ß), in the presence or absence of compound D at the concentration range of 1 to 100 µM. After 24 hours, the cells were analyzed for the expressions of MMPs, IL-1ß and interleukin-1ß-converting enzyme (ICE) by RT-PCR. MMPs activities in the culture media were analyzed by zymographic techniques. Dexamethasone was used as the positive control. It was found that compound D at the concentration of 10 -100 M significantly decreased the mRNA expressions of MMP-1, -2, -3, and -13 which was induced by IL-1ß (P<0.05) concomitantly with a decrease in activities of these MMPs in the culture media. An increase in the mRNA expression of IL-1ß and ICE was also suppressed by compound D. The results suggest that the potent activities of this compound may be involved in the reduction of IL-1ß protein synthesis in both pro-form and active form which played an important role in up-regulation of MMPs. This study first revealed the chondroprotective activity of Z. cassumunar in the transcriptional level by suppressing cytokine-induced catabolic genes which caused cartilage erosion in RA.

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“…The plant's anti-rheumatic actions are attributed to suppressing prostaglandin synthesis through the inhibition of cyclooxygenase-1 and cyclooxygenase-2 (Grzanna et al 2005). Moreover, our previous studies have indicated that A. galanga hexane extract possesses anti-inflammatory effects on IL-1β-induced inflammation in synovial fibroblasts and cartilage explants (Pothacharoen et al 2006). Thus, in this study, we examined the characteristics of Figure 2.…”

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confidence: 98%

Alpinia galanga extracts downregulate interleukin-1β-induced matrix metalloproteinases expression in human synovial fibroblasts

Pothacharoen

Choocheep

Phitak

et al. 2010

In Vitro Cell.Dev.Biol.-Animal

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Alpinia galanga has been used as alternative medicine for anti-rheumatic activities. However, the precise action of the extract on arthritic diseases is not yet fully understood. In this study, we investigated the effects of A. galanga extracts on the expression of genes involved in catabolic activities in an interleukin-1β (IL-1β)-induced human synovial fibroblast as an inflammatory model. Confluent primary human synovial fibroblasts were treated for 24 h with A. galanga hexane extracts in the presence of recombinant human IL-1β. MMPs in the culture medium were monitored by gelatin zymography. Total RNA was isolated from the cell lysate and analyzed via semi-quantitative RT-PCR. After treatment with A. galanga extracts, MMP-2 activity in the culture medium was significantly reduced. In addition, MMP-1, MMP-3, MMP-13, and Cox-2 expression were downregulated. These data suggest that the decrease of gene expression and production of MMPs in synovial fibroblasts against inflammatory stimuli could be due to the effects of the A. galanga extracts. Therefore, A. galanga extracts might be a promising therapeutic agent for arthritis.

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Effect of Alpinia galanga extract on cartilage degradation and on gene expression in human chondrocyte and synovial fibroblast metabolism

Cited by 10 publications

References 32 publications

In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA

In vitro suppression of the MMP-3 gene in normal and cytokine-treated human chondrosarcoma using small interfering RNA

Active Compound of <em>Zingiber Cassumunar</em> Roxb. Down-Regulates the Expression of Genes Involved in Joint Erosion in a Human Synovial Fibroblast Cell Line

Alpinia galanga extracts downregulate interleukin-1β-induced matrix metalloproteinases expression in human synovial fibroblasts

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