Effect of Adhered Bacteria on the Binding of Acanthamoeba to Hydrogel Lenses

Zooming in on the intracellular microbiome composition of bacterivorous Acanthamoeba isolates

“…However, the study found a distinct bacterial microbiome in amoebae compared with the microbiomes present in their soil habitat [ 64 ]. Our study, similar to previous findings [ 65 , 66 ], demonstrates a higher prevalence of Gram-negative bacteria in all isolates, suggesting a preference of Acanthamoeba spp. for Gram-negative bacteria.…”

Zooming in on the intracellular microbiome composition of bacterivorous Acanthamoeba isolates

“…However, the study found a distinct bacterial microbiome in amoebae compared with the microbiomes present in their soil habitat [ 64 ]. Our study, similar to previous findings [ 65 , 66 ], demonstrates a higher prevalence of Gram-negative bacteria in all isolates, suggesting a preference of Acanthamoeba spp. for Gram-negative bacteria.…”

Effect of a Multipurpose Contact Lens Solution on the Survival and Binding of Acanthamoeba Species on Contact Lenses Examined With a No-Rub Regimen

“…20 The study data further support observations that the binding of amoebae to contact lenses differs from the irreversible primary adhesion to lenses reported for bacteria, notably Pseudomonas aeruginosa. [21][22][23] The observations on RMP susceptibilities of Acanthamoeba coupled with the rather loose attachment to the lens may explain further the observations of Stevenson and Seal 6 on an apparent decrease in amoebic keratitis in the United Kingdom after the introduction and use of multipurpose solutions. Acanthamoeba species are ubiquitous in nature, and they should be expected to occur in natural water and certain domestic tap waters.…”

Coinfection with Acanthamoeba and Pseudomonas in Contact Lens–Associated Keratitis

“…2Y4 This is in marked contrast to reports from other Southeast Asian countries like Singapore, where most cases of Acanthamoeba keratitis are related to contact lens wear. 5,6 It has been noted that when cells of Acanthamoeba species were incubated with P. aeruginosa and Staphylococcus epidermidis in a contact lens system, Acanthamoeba showed significantly increased binding with Pseudomonas but not with S. epidermidis. 6 Both Acanthamoeba and P, aeruginosa share many characteristics as eye pathogens.…”

“…5,6 It has been noted that when cells of Acanthamoeba species were incubated with P. aeruginosa and Staphylococcus epidermidis in a contact lens system, Acanthamoeba showed significantly increased binding with Pseudomonas but not with S. epidermidis. 6 Both Acanthamoeba and P, aeruginosa share many characteristics as eye pathogens. Both can adhere to, colonize, and invade injured corneal tissue, 7 can produce tissue-destructive enzymes, 8,9 and have been recovered individually as contaminants of contact lens care systems.…”

Zooming in on the intracellular microbiome composition of bacterivorous Acanthamoeba isolates

“…However, the study found a distinct bacterial microbiome in amoebae compared with the microbiomes present in their soil habitat [ 64 ]. Our study, similar to previous findings [ 65 , 66 ], demonstrates a higher prevalence of Gram-negative bacteria in all isolates, suggesting a preference of Acanthamoeba spp. for Gram-negative bacteria.…”

Effect of a Multipurpose Contact Lens Solution on the Survival and Binding of Acanthamoeba Species on Contact Lenses Examined With a No-Rub Regimen

“…20 The study data further support observations that the binding of amoebae to contact lenses differs from the irreversible primary adhesion to lenses reported for bacteria, notably Pseudomonas aeruginosa. [21][22][23] The observations on RMP susceptibilities of Acanthamoeba coupled with the rather loose attachment to the lens may explain further the observations of Stevenson and Seal 6 on an apparent decrease in amoebic keratitis in the United Kingdom after the introduction and use of multipurpose solutions. Acanthamoeba species are ubiquitous in nature, and they should be expected to occur in natural water and certain domestic tap waters.…”

Coinfection with Acanthamoeba and Pseudomonas in Contact Lens–Associated Keratitis

“…2Y4 This is in marked contrast to reports from other Southeast Asian countries like Singapore, where most cases of Acanthamoeba keratitis are related to contact lens wear. 5,6 It has been noted that when cells of Acanthamoeba species were incubated with P. aeruginosa and Staphylococcus epidermidis in a contact lens system, Acanthamoeba showed significantly increased binding with Pseudomonas but not with S. epidermidis. 6 Both Acanthamoeba and P, aeruginosa share many characteristics as eye pathogens.…”

“…5,6 It has been noted that when cells of Acanthamoeba species were incubated with P. aeruginosa and Staphylococcus epidermidis in a contact lens system, Acanthamoeba showed significantly increased binding with Pseudomonas but not with S. epidermidis. 6 Both Acanthamoeba and P, aeruginosa share many characteristics as eye pathogens. Both can adhere to, colonize, and invade injured corneal tissue, 7 can produce tissue-destructive enzymes, 8,9 and have been recovered individually as contaminants of contact lens care systems.…”

Zooming in on the intracellular microbiome composition of bacterivorous Acanthamoeba isolates

“…However, the study found a distinct bacterial microbiome in amoebae compared with the microbiomes present in their soil habitat [ 64 ]. Our study, similar to previous findings [ 65 , 66 ], demonstrates a higher prevalence of Gram-negative bacteria in all isolates, suggesting a preference of Acanthamoeba spp. for Gram-negative bacteria.…”

Effect of a Multipurpose Contact Lens Solution on the Survival and Binding of Acanthamoeba Species on Contact Lenses Examined With a No-Rub Regimen

“…20 The study data further support observations that the binding of amoebae to contact lenses differs from the irreversible primary adhesion to lenses reported for bacteria, notably Pseudomonas aeruginosa. [21][22][23] The observations on RMP susceptibilities of Acanthamoeba coupled with the rather loose attachment to the lens may explain further the observations of Stevenson and Seal 6 on an apparent decrease in amoebic keratitis in the United Kingdom after the introduction and use of multipurpose solutions. Acanthamoeba species are ubiquitous in nature, and they should be expected to occur in natural water and certain domestic tap waters.…”

Coinfection with Acanthamoeba and Pseudomonas in Contact Lens–Associated Keratitis

“…2Y4 This is in marked contrast to reports from other Southeast Asian countries like Singapore, where most cases of Acanthamoeba keratitis are related to contact lens wear. 5,6 It has been noted that when cells of Acanthamoeba species were incubated with P. aeruginosa and Staphylococcus epidermidis in a contact lens system, Acanthamoeba showed significantly increased binding with Pseudomonas but not with S. epidermidis. 6 Both Acanthamoeba and P, aeruginosa share many characteristics as eye pathogens.…”

“…5,6 It has been noted that when cells of Acanthamoeba species were incubated with P. aeruginosa and Staphylococcus epidermidis in a contact lens system, Acanthamoeba showed significantly increased binding with Pseudomonas but not with S. epidermidis. 6 Both Acanthamoeba and P, aeruginosa share many characteristics as eye pathogens. Both can adhere to, colonize, and invade injured corneal tissue, 7 can produce tissue-destructive enzymes, 8,9 and have been recovered individually as contaminants of contact lens care systems.…”