Effect of acute heat stress on heat shock protein 70 messenger RNA and on heat shock protein expression in the liver of broilers

Gabriel, Jane Eyre; Ferro, J. A.; Stefani, Rosane M. P.; Ferro, Maria Inês Tiraboschi; Gomes, Suely L.; Macari, Marcos

doi:10.1080/00071669608417875

Cited by 51 publications

(25 citation statements)

References 25 publications

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“…The results coincide with those of Gabriel et al (1996) and Dionello et al (2001). In addition, mRNA expression of chicken HSP70 was tissue dependent, which agrees with the results of Givisiez et al (2003).…”

Section: Discussionsupporting

confidence: 82%

Promoter methylation negatively correlated with mRNA expression but not tissue differential expression after heat stress

Gan

Zhang²,

et al. 2013

Genet. Mol. Res.

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ABSTRACT. DNA methylation plays a central role in gene expression. In this study, we detected the promoter methylation pattern of the chicken heat shock protein 70 (HSP70) gene and its association with messenger RNA (mRNA) expression before and after heat shock. The results showed that mRNA expression increased in response to heat stress and peaked at 3 h before dropping. Hypomethylation of the HSP70 promoter occurred in all of the groups studied, but the difference between groups within tissue type was not significant. The DNA methylation level of the control and the 6-h treatment groups was slightly higher than that of the 3-h treatment group in brain tissue and leg muscle. Correlation analysis between mRNA expression and DNA methylation of HSP70 showed that DNA methylation was negatively associated with mRNA expression in leg muscle (P = 0.0124), indicating that DNA methylation may be negatively associated with the expression of HSP70, although the difference was not significant. We concluded that the expression of HSP70 is heat inducible and tissue dependent and that heat induction may correlate with DNA methylation pattern in the HSP70 promoter, whereas tissue dependence is unrelated to DNA methylation pattern.

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Section: Discussionsupporting

confidence: 82%

Promoter methylation negatively correlated with mRNA expression but not tissue differential expression after heat stress

Gan

Zhang²,

et al. 2013

Genet. Mol. Res.

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“…The present study found that the transcriptional levels of HSF1 and HSP70 as well as the protein expression increased in the normal muscles of the T and T/W groups. Gabriel et al (1996) found that acute heat stress induces an increase in HSP70 mRNA in broiler liver, which is similar to that of mammals. Delezie et al (2007) indicated that HSP70 mRNA increases in the liver of broilers when transported at high stocking densities.…”

Section: Discussionmentioning

confidence: 54%

Expression of heat shock protein 70 in transport-stressed broiler pectoralis major muscle and its relationship with meat quality

Xing

Wang

Han

et al. 2017

Animal

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Omics research has indicated that heat shock protein 70 (HSP70) is a potential biomarker of meat quality. However, the specific changes and the potential role of HSP70 in postmortem meat quality development need to be further defined. In this study, Arbor Acres broiler chickens (n = 126) were randomly categorized into three treatment groups of unstressed control (C), 0.5-h transport (T) and subsequent water shower spray following transport (T/W). Each treatment consisted of six replicates with seven birds each. The birds were transported according to a designed protocol. The pectoralis major (PM) muscles of the transport-stressed broilers were categorized as normal and pale, soft and exudative (PSE)-like muscle samples according to L* and pH 24 h values to test the expression and location of HSP70. Results revealed that the activities of plasma creatine kinase and lactate dehydrogenase increased significantly ( P < 0.05) in normal and PSE-like muscle samples after transportation. The mRNA expression of HSP70 in normal muscle samples increased significantly ( P < 0.05) compared with that in the controls after stress. The protein expression of HSP70 increased significantly in normal muscle samples and decreased significantly ( P < 0.05) in PSE-like muscles. Immuno-fluorescence showed that HSP70 was present in the cytoplasm and on surface membranes of PM muscle cells in the normal samples following stress. Meanwhile, HSP70 was present on the surface membranes and extracellular matrix but was barely visible in the cytoplasm of the PSE-like samples. Principal component analysis showed high correlations between HSP70 and meat quality and stress indicators. In conclusion, this research suggests that the variation in HSP70 expression may provide a novel insight into the pathways underlying meat quality development.

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“…Losses due to mortality and impaired performance of the birds were decreased in acclimated birds, and a greater heat resistance was achieved by exposing birds to cycling temperatures (May et al 1987;Davis et al 1991) or neonatal heat stress (Arjona et al 1988(Arjona et al , 1990Yahav and Hurwitz 1996). Molecular mechanisms responsible for improved thermotolerance have not been fully elucidated (Gabriel et al 1996).It is known that heat stress and other kinds of stressors (e.g., anoxia and heavy metal ions) rapidly induce the synthesis of heat shock proteins (Hsp), which, in a normal envi- …”

mentioning

confidence: 99%

Heat or cold chronic stress affects organ weights and Hsp70 levels in chicken embryos

Givisiez¹,

Silva²,

Mazzi³

et al. 2001

Can. J. Anim. Sci.

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M. 2001. Heat or cold chronic stress affects organ weights and Hsp70 levels in chicken embryos. Can. J. Anim. Sci. 81: 83-87. This study was carried out with the objective of evaluating the effect of heat (38.8°C) or cold (35.8°C) stress on chicken embryo development and tissues Hsp70 levels, after the 13th day of incubation. Embryo weight (percent egg weight), organ weight (percent embryo weight) and Hsp70 levels (ng Hsp70 µg -1 total protein) in different tissues (liver, breast muscle, heart, lungs, brain and kidney) were studied at the end of incubation. Cold stress induced a lower embryo weight and lower kidney and lungs weights, whereas heart and liver were lighter in heat-stressed embryos. An interaction between temperature and age was obtained only for Hsp70 levels in kidney and heart. Cold-stressed embryos showed higher Hsp70 levels in the brain, lungs and liver; a decrease in brain and breast muscle Hsp70 levels was seen from the 19th to 20th days in control embryos. Hsp70 levels increased with age in kidneys of control embryos and in heart of heat-and cold-stressed embryos. In conclusion, this study showed that chicken embryo organ weights are affected by incubation temperature, and that Hsp70 expression is tissue dependent (higher levels being seen in the brain) being cold-stress more effective in increasing Hsp70 levels in most studied tissues.Key words: Chicken embryo, development, Hsp70, incubation temperature Givisiez, P. E. N., da Silva, M. M., Mazzi, C. M., Ferro, M. I. T., Ferro, J. A., Gonzales, E. et Macari, M. 2001. Le stress chronique causé par la chaleur ou le froid modifie le poids des organes et la concentration de Hsp70 chez l'embryon du poulet. Can. J. Anim. Sci. 81: 83-87. L'étude devait servir à évaluer les effets de la chaleur (38,8°C) ou du froid (35,8°C) sur l'embryogenèse du poulet et sur la concentration de Hsp70 dans les tissus après 13 jours d'incubation. On a mesuré le poids de l'embryon (% du poids de l'oeuf), le poids des organes (% du poids de l'embryon) et la concentration de Hsp70 (ng Hsp70 µg -1 protéines totales) dans divers tissus (foie, muscles de la poitrine, coeur, poumons, encéphale et reins) à la fin de l'Incubation. Le stress causé par le froid diminue le poids de l'embryon et celui des reins et des poumons, tandis que c'est le coeur et le foie qui sont plus petits chez les embryons soumis au stress causé par la chaleur. Une interaction entre la température et l'âge n'a été observée qu'au niveau de concentration de Hsp70 dans les reins et le coeur. Les embryons soumis au stress causé par le froid présentaient plus de Hsp70 dans l'encéphale, les poumons et le foie; chez les embryons témoins, la concentration de Hsp70 dans le cerveau et les muscles de la poitrine diminue entre le 19 e et le 20 e jour d'incubation. La concentration de Hsp70 s'accroît avec l'âge dans les reins des embryons témoins et dans le coeur des embryons soumis à l'un ou l'autre stress thermique. Les auteurs en concluent que chez l'embryon de poulet, le poids des organes est affecté par la te...

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Effect of acute heat stress on heat shock protein 70 messenger RNA and on heat shock protein expression in the liver of broilers

Cited by 51 publications

References 25 publications

Promoter methylation negatively correlated with mRNA expression but not tissue differential expression after heat stress

Promoter methylation negatively correlated with mRNA expression but not tissue differential expression after heat stress

Expression of heat shock protein 70 in transport-stressed broiler pectoralis major muscle and its relationship with meat quality

Heat or cold chronic stress affects organ weights and Hsp70 levels in chicken embryos

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