Effect of a hypo-osmotic shock on the volume and the ion content of rat kidney cortex slices

Mechanisms in Volume Regulation in Ehrlich Ascites Tumor Cells

“…In some vertebrate cell types a net loss of free amino acids and related compounds dur ing R VD contributes to the volume recovery, similar to the findings reported for volume regulation in all invertebrate phyla studied so far [see Gilles, 1979;Seel et al, 1980;Pierce, 1982], The dominating part of the net loss of osmotically active nonelectrolytes is ac counted for by taurine (2-aminoethanesulfonic acid), glycine, alanine, and other small, non-essential amino acids. This decrease ac counts in Ehrlich ascites cells for approxi mately 30% of the total decrease in osmoti cally active substances [Hoffmann and Hcndil, 1976].…”

Mechanisms in Volume Regulation in Ehrlich Ascites Tumor Cells

“…In some vertebrate cell types a net loss of free amino acids and related compounds dur ing R VD contributes to the volume recovery, similar to the findings reported for volume regulation in all invertebrate phyla studied so far [see Gilles, 1979;Seel et al, 1980;Pierce, 1982], The dominating part of the net loss of osmotically active nonelectrolytes is ac counted for by taurine (2-aminoethanesulfonic acid), glycine, alanine, and other small, non-essential amino acids. This decrease ac counts in Ehrlich ascites cells for approxi mately 30% of the total decrease in osmoti cally active substances [Hoffmann and Hcndil, 1976].…”

Proceedings of the Physiological Society, 25‐26 March 1986, University College London/Middlesex Meeting: Communications

“…However, although NPS have been identified in mammalian kidneys (Ullrich & Jarausch, 1956;Robinson, Owen & Schmidt-Nielsen, 1966) it is uncertain whether they play a role in cell volume regulation. Seel, Rorive, Pequeux & Gilles (1980) (a) keeping NaCl constant (250 mmol/l) and varying urea, or (b) keeping urea constant (500 mmol/l) and varying NaCl, showed that cell volume maintenance was more efficient, and IC NPS far higher, when urea rather than NaCl was the principal osmotic additive. Although volume maintenance may partly be ascribed to the greater permeancy of urea relative to NaCl, there may also be a contributory effect of ideogenic NPS which is dependent upon urea (the major medullary osmolyte during antidiuresis), since equimolar replacement of urea with relatively impermeant sucrose or sorbitol did not affect NPS.…”

Amino acid transport and cell volume regulation in Ehrlich ascites tumour cells.

“…The role of amino acids in volume regulatory responses has been demonstrated * The order of names is alphabetical in many invertebrates (see Gilles, 1979) and several vertebrates (see Hoffmann, 1977). With respect to mammalian cells, the involvement of amino acids has so far only been demonstrated in Ehrlich ascites tumour cells (Hoffmann & Hendil, 1976) and rat kidney cortex (Sell, Rorive, Pequeux & Gilles, 1980). In Ehrlich cells, it has been demonstrated that the loss of KCl is a consequence of very selective increases in the permeabilities to K+ and Cl-followed by a decrease in the permeability to Na+ (Hoffmann, 1978).…”

Mechanisms in Volume Regulation in Ehrlich Ascites Tumor Cells

“…In some vertebrate cell types a net loss of free amino acids and related compounds dur ing R VD contributes to the volume recovery, similar to the findings reported for volume regulation in all invertebrate phyla studied so far [see Gilles, 1979;Seel et al, 1980;Pierce, 1982], The dominating part of the net loss of osmotically active nonelectrolytes is ac counted for by taurine (2-aminoethanesulfonic acid), glycine, alanine, and other small, non-essential amino acids. This decrease ac counts in Ehrlich ascites cells for approxi mately 30% of the total decrease in osmoti cally active substances [Hoffmann and Hcndil, 1976].…”

Proceedings of the Physiological Society, 25‐26 March 1986, University College London/Middlesex Meeting: Communications

“…However, although NPS have been identified in mammalian kidneys (Ullrich & Jarausch, 1956;Robinson, Owen & Schmidt-Nielsen, 1966) it is uncertain whether they play a role in cell volume regulation. Seel, Rorive, Pequeux & Gilles (1980) (a) keeping NaCl constant (250 mmol/l) and varying urea, or (b) keeping urea constant (500 mmol/l) and varying NaCl, showed that cell volume maintenance was more efficient, and IC NPS far higher, when urea rather than NaCl was the principal osmotic additive. Although volume maintenance may partly be ascribed to the greater permeancy of urea relative to NaCl, there may also be a contributory effect of ideogenic NPS which is dependent upon urea (the major medullary osmolyte during antidiuresis), since equimolar replacement of urea with relatively impermeant sucrose or sorbitol did not affect NPS.…”

Amino acid transport and cell volume regulation in Ehrlich ascites tumour cells.

“…The role of amino acids in volume regulatory responses has been demonstrated * The order of names is alphabetical in many invertebrates (see Gilles, 1979) and several vertebrates (see Hoffmann, 1977). With respect to mammalian cells, the involvement of amino acids has so far only been demonstrated in Ehrlich ascites tumour cells (Hoffmann & Hendil, 1976) and rat kidney cortex (Sell, Rorive, Pequeux & Gilles, 1980). In Ehrlich cells, it has been demonstrated that the loss of KCl is a consequence of very selective increases in the permeabilities to K+ and Cl-followed by a decrease in the permeability to Na+ (Hoffmann, 1978).…”

Mechanisms in Volume Regulation in Ehrlich Ascites Tumor Cells

“…In some vertebrate cell types a net loss of free amino acids and related compounds dur ing R VD contributes to the volume recovery, similar to the findings reported for volume regulation in all invertebrate phyla studied so far [see Gilles, 1979;Seel et al, 1980;Pierce, 1982], The dominating part of the net loss of osmotically active nonelectrolytes is ac counted for by taurine (2-aminoethanesulfonic acid), glycine, alanine, and other small, non-essential amino acids. This decrease ac counts in Ehrlich ascites cells for approxi mately 30% of the total decrease in osmoti cally active substances [Hoffmann and Hcndil, 1976].…”

Proceedings of the Physiological Society, 25‐26 March 1986, University College London/Middlesex Meeting: Communications

“…However, although NPS have been identified in mammalian kidneys (Ullrich & Jarausch, 1956;Robinson, Owen & Schmidt-Nielsen, 1966) it is uncertain whether they play a role in cell volume regulation. Seel, Rorive, Pequeux & Gilles (1980) (a) keeping NaCl constant (250 mmol/l) and varying urea, or (b) keeping urea constant (500 mmol/l) and varying NaCl, showed that cell volume maintenance was more efficient, and IC NPS far higher, when urea rather than NaCl was the principal osmotic additive. Although volume maintenance may partly be ascribed to the greater permeancy of urea relative to NaCl, there may also be a contributory effect of ideogenic NPS which is dependent upon urea (the major medullary osmolyte during antidiuresis), since equimolar replacement of urea with relatively impermeant sucrose or sorbitol did not affect NPS.…”

Amino acid transport and cell volume regulation in Ehrlich ascites tumour cells.

“…The role of amino acids in volume regulatory responses has been demonstrated * The order of names is alphabetical in many invertebrates (see Gilles, 1979) and several vertebrates (see Hoffmann, 1977). With respect to mammalian cells, the involvement of amino acids has so far only been demonstrated in Ehrlich ascites tumour cells (Hoffmann & Hendil, 1976) and rat kidney cortex (Sell, Rorive, Pequeux & Gilles, 1980). In Ehrlich cells, it has been demonstrated that the loss of KCl is a consequence of very selective increases in the permeabilities to K+ and Cl-followed by a decrease in the permeability to Na+ (Hoffmann, 1978).…”