Effect of a High Protein Intake on Calcium Metabolism in the Rat

Bell, Roma R.; Engelmann, D.; Sie, Ten-Lin; Draper, H. H.

doi:10.1093/jn/105.4.475

Cited by 72 publications

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“…However, in these rats, the oil content in the diet had a significant effect on fecal Ca excretion and the protein content in the diet had a significant effect on urinary Ca excretion. Several previous studies on humans (11,12) and rats (13)(14)(15) reported that urinary calcium excretion increased as dietary protein intake increased. Azami et al (3) and Kitano et al (4) reported the relationship between protein intake and urinary calcium excretion in multiparous rats.…”

Section: Discussionmentioning

confidence: 93%

Effect of the Protein and Oil Contents on Calcium Utilization in the Diets of Female Fischer Rats That Were Fed by Three Different Feeding Methods

Ohtsuka

Kitano

Maeda

et al. 2006

J Nutr Sci Vitaminol

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SummaryWe studied the effects of varying the protein and oil contents in the diet and three different feeding methods on the utilization of dietary calcium (Ca) in female Fischer rats. The experimental diets were based on the AIN-76 diet. Rats were fed one of nine experimental diets containing different levels of protein and oil. The experimental diets contained 10, 20 or 40% of protein (milk casein) and 5, 10 or 20% of soybean oil. The three meal feeding methods were ad libitum feeding, pair feeding and adjustable feeding. The experimental diets that were given by prepared feeding were supplemented with a mineral and vitamin solution. The effects of the protein and oil contents in the diet were analyzed by two-way ANOVA. Among rats that were fed ad libitum, the oil content in the diet affected the degree of mineral and vitamin intake. Among the rats fed ad libitum, the oil content in the diet had a significant effect on the level of Ca intake, although it did not have a significant effect on the level of energy intake. For pair feeding, the feeding volume was limited in each group; therefore, there was a significant difference in energy intake and there was no significant difference in Ca intake among each diet group. For adjustable feeding, there was considerable mineral and vitamin intake, which effected the reduction of feeding volume depending on the oil volume in the diet. There were no significant differences in the energy, Ca and other mineral and vitamin intakes among the nine groups that were fed using adjustable feeding. In analyzing two-way ANOVA, in which the parameters were the contents of protein and oil in the experimental diets, there were differences in Ca utilization among rats that were fed using the three feeding methods. This result was shown to reflect on the difference as energy, mineral including Ca, and vitamin intake. However, the protein content in the diet had a significant effect on urinary Ca excretion in all three feeding methods. In addition, it was clear that the intake of protein and oil affected kidney calcification in all three feeding methods. The AIN Experimental Diet Committee reported that kidney calcification was found among rats that were fed the AIN-76 diet and that one of the causes of kidney calcification was the Ca/P ratio in this diet. The protein and oil contents in the diets had significant effects on the degree of kidney calcification among rats that were fed by pair feeding or adjustable feeding. The results of this study suggest that the protein and oil contents in the diet play an important role in kidney calcification. Key Words calcium balance, protein intake, oil intake, kidney calcification, feeding method There have been many nutrition studies on the bioavailability of minerals including calcium, phosphorus (P) and magnesium (Mg) balance studies in experimental animals such as rats. We previously studied the effect of varying the level of protein intake on calcium utilization and found that a high-protein diet increased the absorption and urinary excreti...

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Section: Discussionmentioning

confidence: 93%

Effect of the Protein and Oil Contents on Calcium Utilization in the Diets of Female Fischer Rats That Were Fed by Three Different Feeding Methods

Ohtsuka

Kitano

Maeda

et al. 2006

J Nutr Sci Vitaminol

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“…induction of osteoporosis, a number of studies have been conducted to observe the effect of a high protein diet on bone and Ca metabolism (2)(3)(4)(5)(6)(7)(8)(9). In our previous report (9), it was clarified that urinary Ca excretion was increased and apparent Ca absorption was decreased when growing rats were fed a high protein diet.…”

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confidence: 99%

“…Reduced retention of Ca might induce impaired bone formation and resorption. Draper and co-workers reported that a high protein diet did not increase the bone resorption using 45Ca kinetics (3,6,8) but radiographic evidence of a reduction in metaphyseal bone density indicating the depression of bone formation was found in rats fed a high sulfur-containing amino acids diet (7).…”

mentioning

confidence: 99%

“…showed that a high protein diet decreased the incorporation of 45Ca into the bone matrix at the stage of osteogenesis and suggested that the decrease in bone formation was induced by the restricted availability of Ca at the site of mineralization (5). Weiss et al used an 80% casein-included diet as a high protein diet (5), while other investigators commonly used 35-40% protein diet as a high protein diet (3,(8)(9)(10). It was reported that a dietary protein level changed the degree of hypercalciuria (10).…”

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confidence: 99%

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Effects of a high protein diet on bone formation and calcium metabolism in rats.

Funaba

Kawashima

Yano

et al. 1990

Journal of Nutritional Science and Vitaminology, J Nutr Sci Vit

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SummaryThe effects of a high protein diet on bone formation and calcium (Ca) metabolism were evaluated in rats using an ectopic endo chondral bone induction model. A control diet (18% casein) or a high protein diet (18% casein+20% lactalbumin) was given to 50-day-old rats. Ten days after the feeding of the experimental diet, rats were intramuscularly implanted with demineralized bone powder (day 0) . On day 14 and day 21, the implanted bone powder was harvested , and blood and urine samples were also obtained. Urinary Ca excretion was not increased on day 12-14; however, it was elevated on day 19-21 in rats fed the high protein diet compared with rats fed the control diet. The high protein diet remarkably stimulated urinary sulfate excretion in both sampling periods, which reflected dietary sulfur-containing amino acids contents. Also, rats fed the high protein diet exhibited a decrease in serum Ca concentrations. There was little difference in Ca contents and the activities of alkaline phosphatase and acid phosphatase in the implants between control group and high protein diet group on day 14 and day 21. Histological examination in the implanted demineralized bone powder on day 14 indicated only cartilage in rats fed the high protein diet in contrast to the occurrences of osteogenesis and remodeling in those fed the control diet. Retarded bone formation in rats fed the high protein diet might be owing to, in part at least, a restricted amount of Ca utilized at the stage of cartilage calcification. Key Words high protein diet, ectopic bone formation, urinary calcium excretion, rat Since a report by Wachman and Bernstein in 1968 (1) that an increase in protein intake caused hypercalciuria, leading to a body calcium (Ca) loss and an 559

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“…Em estudo realizado com ratos, Bell et al (1975) relataram que os animais que consumiram dietas com altos níveis de proteína apresentaram hipercalciúria e diminuição de cálcio fecal. Segundo esses autores a redução de cálcio nas fezes estaria associada ao aumento da absorção intestinal de cálcio ou da reabsorção óssea ou ainda de ambos os fatores.…”

Section: Feed Conversionunclassified

Desempenho, rendimento de carcaça e excreção de cálcio de frangos de corte alimentados com diferentes níveis de aminoácidos e cálcio no período de 22 a 42 dias de idade

Araújo

Artoni

Araujo³

et al. 2002

R. Bras. Zootec.

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RESUMO -Um experimento foi conduzido com o objetivo de avaliar diferentes níveis de aminoácidos e de cálcio para frangos de corte no período de 22 a 42 dias de idade. Avaliou-se o desempenho, o rendimento de carcaça e a excreção de cálcio. Foram utilizadas 540 aves, machos, da marca comercial Cobb, distribuídas em delineamento inteiramente casualizado, em esquema fatorial 3x2, ou seja, 3 níveis de aminoácidos (metionina, metionina+cistina, lisina e treonina -100%, 125% e 150% NRC,1994) e 2 níveis de cálcio (75% e 100% NRC,1994), com 3 repetições de 30 aves cada. Os aminoácidos estudados foram metionina, metionina+cistina, lisina e treonina. O desempenho das aves foi avaliado pelo peso vivo, ganho de peso, consumo de ração e conversão alimentar. O rendimento de carcaça e a percentagem de cada parte foram avaliados de 2 aves de cada repetição, escolhidas ao acaso, perfazendo um total de 6 animais por tratamento. O rendimento de carcaça foi realizado após o abate levando-se em consideração a percentagem de peito, pernas (coxa+sobrecoxa), asas, dorso, cabeça+pescoço e pés em relação à carcaça eviscerada. Além disso, analisou-se o cálcio excretado pelas aves. Para tanto, utilizou-se 72 aves, alojadas em gaiolas, seguindo o mesmo delineamento descrito anteriormente. Não houve interação entre os fatores estudados para as características avaliadas. As que consumiram dietas com 125% e 150% de aminoácidos apresentaram melhor conversão alimentar. O rendimento de peito foi afetado pelos níveis de cálcio.Palavras-chave: aminoácidos, cálcio, desempenho, frangos de corte, rendimento de carcaça Performance, Carcass Yield and Calcium Excretion of Broilers Fed Diets withDifferent Amino Acids and Calcium Levels, from 22 to 42 Days of Age ABSTRACT -One experiment was conducted to evaluate different amino acid (AA) and calcium (Ca) levels for broilers from 22 to 42 days of age. Evaluated the performance, carcass yield and calcium excretion. Five forty hundred birds, Male broilers Cobb, were randomly assigned to 6 treatments in a 3x2 factorial arrangement, considering the factors AA (100, 125 and 150% NRC, 1994) and Ca (75 and 100% NRC, 1994), in three replications of 30 birds each. The studied AA were methionine, methionine+cystine, lysine and threonine. The performance of the birds was analyzed according to body weight, weight gain, feed intake and feed conversion. Carcass yield and the percentage of each part were evaluated from 2 birds randomly taken from each pen at 42 days of age, in a total of 6 birds per treatment. Carcass yield was evaluated just after the slaughter in relation to individual percentage carcass as well as breast, legs (thigh and drumstick), wing, back, head+neck and feet. Besides, the excreted calcium of the birds was analyzed. For so much, it was used 72 birds, in experiment, housed in cages, following the same design described previously. It was not found interaction between the factors studied. The birds fed diet 125% and 150% AA showed improvement in feed conversion. Breast yield was affected by dietary...

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Effect of a High Protein Intake on Calcium Metabolism in the Rat

Cited by 72 publications

References 0 publications

Effect of the Protein and Oil Contents on Calcium Utilization in the Diets of Female Fischer Rats That Were Fed by Three Different Feeding Methods

Effect of the Protein and Oil Contents on Calcium Utilization in the Diets of Female Fischer Rats That Were Fed by Three Different Feeding Methods

Effects of a high protein diet on bone formation and calcium metabolism in rats.

Desempenho, rendimento de carcaça e excreção de cálcio de frangos de corte alimentados com diferentes níveis de aminoácidos e cálcio no período de 22 a 42 dias de idade

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