Effect of 2,4-Thiazolidinedione on Limousin Cattle Growth and on Muscle and Adipose Tissue Metabolism

Abstract

The main adipogenic transcription factor PPARγ possesses high affinity to 2,4-TZD, a member of the Thiazolidinedione family of insulin-sensitizing compounds used as adipogenic agents. We evaluated 2,4-TZD's effect on bovine growth and PPAR tissue expression. Seventeen Limousin bulls (18 month-old; 350 kg body weight (BW)) were assigned into 2 treatments: control and 2,4-TZD (8 mg/70 kg BW) and were fed until bulls reached 500 kg BW. They were weighed and their blood was sampled. DNA, RNA, and protein were determined in liver; skeletal muscle; subcutaneous (SC), omental, perirenal adipose tissues (AT) to determine protein synthesis rate and cellular size. Expression of PPAR mRNA was measured in liver and muscle (PPARα, -δ, and -γ) and SC adipose tissue (γ) by real-time PCR. No significant differences were found (P > 0.1) in weight gain, days on feed, and carcass quality. Muscle synthesis was greater in controls (P < 0.05); cell size was larger with 2,4-TZD (P < 0.05). PPARα, -δ, and -γ expressions with 2,4-TZD in liver were lower (P < 0.01) than in muscle. No differences were found for PPARγ mRNA expression in SCAT. The results suggest the potential use of 2,4-TZD in beef cattle diets, because it improves AT differentiation, liver, and muscle fatty acid oxidation that, therefore, might improve energy efficiency.

Dietary Supplementation with Pioglitazone Hydrochloride and Resveratrol Improves Meat Quality and Antioxidant Capacity of Broiler Chickens

“…More adipocytes and larger cell sizes have been observed in mice supplemented with PGZ [13,14,18]. According to previous reports, PGZ has the potential to promote fat deposition in animal muscle [19,20]. Consistent with these findings, our previous studies confirmed that dietary supplementation with 15 mg/kg PGZ can increase the IMF in the longissimus thoracis muscle of pigs [21,22].…”

“…From the present and some previous studies [13][14][15][16][17][18][19][20][21][22], we can conclude that dietary PGZ can increase muscle fat deposition and improve the meat quality of food animals. It has potential as a new functional feed additive.…”

Dietary supplementation with pioglitazone hydrochloride improves intramuscular fat, fatty acid profile, and antioxidant ability of thigh muscle in yellow‐feathered chickens

“…7 In studies of ruminants, intramuscular adipose deposition has already been demonstrated to be increased by dietary supplementation with PGZ. 8 In our early exploration, PGZ also showed a positive effect on IMF and the marbling score of finishing pigs. 9,10 However, it is still unclear whether PGZ supplementation would improve poultry meat quality and increase IMF.…”

“…Pioglitazone hydrochloride (PGZ), a high‐affinity ligand of PPAR γ , therefore interested us . In studies of ruminants, intramuscular adipose deposition has already been demonstrated to be increased by dietary supplementation with PGZ . In our early exploration, PGZ also showed a positive effect on IMF and the marbling score of finishing pigs .…”

Functional Role of PPARs in Ruminants: Potential Targets for Fine-Tuning Metabolism during Growth and Lactation

“…Among the few available studies, it was observed that liver of dairy cows expresses a similar amount of PPARA and PPARD but does not express PPARG [44]. In a recent study where the expression of the three PPAR isotypes was evaluated in liver and muscle of beef bulls, the greatest expression was observed for PPARA , followed by PPARG , with the lowest expression for PPARD in liver, while, the largest expression in muscle was observed for PPARG [71]. This relative distribution among tissues is somewhat comparable to our data (Figure 1(b)).…”