Effect of 17β-estradiol on adhesion of Mytilus galloprovincialis hemocytes to selected substrates. Role of alpha2 integrin subunit

Koutsogiannaki, Sophia; Kaloyianni, Martha

doi:10.1016/j.fsi.2011.04.003

Cited by 11 publications

(6 citation statements)

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“…Genomic (Zhang et al 2012) and transcriptomic (Zhao et al 2012) analyses have suggested that collagen might play an important role in shell formation and soft tissue growth and repair in bivalves. In addition, collagen also appears to play a relevant role in the adhesion and migration of haemocytes to the ECM (Koutsogiannaki and Kaloyianni, 2011) and likely plays a crucial role in the process of cell immunity during inflammatory response (Adams, 2018). Fibulin have been reported…”

Section: Accepted Manuscriptmentioning

confidence: 99%

Gill transcriptomic analysis in fast- and slow-growing individuals of Mytilus galloprovincialis

et al. 2019

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The molecular basis underlying the mechanisms at the origin of growth variation in bivalves is still poorly understood, although several genes have been described as upregulated in fast-growing individuals. In the present study, we reared mussel spat of the species Mytilus galloprovincialis under diets below the pseudofaeces threshold (BP) and above the pseudofaeces threshold (AP). After 3 months, F and S mussels from each condition were selected to obtain 4 experimental groups: FBP, SBP, FAP and SAP. We hypothesized that the nurturing conditions during the growing period would modify the molecular basis of their growth rate differences. To decipher the molecular mechanisms underlying the growth variation, the gill transcriptomes for the four mussel groups were analysed. Gene expression analysis revealed i) a low number (12) of genes differentially expressed in association with diet and ii) 117 genes differentially expressed by the fast-and slow-growing mussels. According to Biological Process GO term analysis transcriptomic differences between the F and S mussels were mainly based on the upregulation of: response to the stimulus, growth and cell activity. Regarding the KEGG terms, carbohydrate metabolism and the Krebs cycle were upregulated in F mussels, whereas biosynthetic processes were upregulated in S mussels. In accordance with their larger gill surface area and higher rates of feeding and growth, the F individuals overexpressed genes in their gill tissues, and these were involved in i) growth (insulin-like growth factors and myostatin); ii) maintenance of the structure and functioning of extracellular matrix (collagen, laminin, fibulin and decorin); iii) filtration and ciliary activity (mucin, fibrocystin, dynein and tilB homolog protein genes); iv) aerobic metabolism (citrate synthase and carbonic anhydrase); and v) the immune-system, probably in association with haemocytes. In contrast, S individuals overexpressed a different series of genes pertaining to immune system (leucine-rich repeat protein and galectin), along with genes involved in the response to cellular stress (Heat shock protein (HSP24) and metalloendopeptidase) as well as anaerobic metabolism (C4-dicarboxylate transporter). These results might suggest that S individuals would have a greater prevalence of pathogens/diseases or a higher susceptibility to the pathogens. Highlights ► Fast-and slow-growing mussel (Mytilus galloprovincialis) spat were found to differentially express 117 genes in gill tissue. ► Fast-growing mussels overexpressed genes involved in response to stimulus,growth and cell activity processes. ► Slow-growing mussels overexpressed genes involved in immune and defence processes. ► Feeding above or below the pseudofaeces production threshold did not exert a relevant effect on the gill transcriptome.

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Section: Accepted Manuscriptmentioning

confidence: 99%

Gill transcriptomic analysis in fast- and slow-growing individuals of Mytilus galloprovincialis

et al. 2019

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“…Although information regarding the evolutionary and functional relationships among invertebrate integrins is not clear, and limited by the few genome-sequenced species, more and more integrin family members are identified in non-model invertebrates, such as in the Mollusca, which is the biggest phylum in the marine animal kingdom with the most species. The molecular aspects of some integrins in mollusks such as Lymnaea stagnalis ( 39 ), Mytilus trossulus ( 40 , 41 ), B. glabrata ( 23 , 42 ), Mytilus galloprovincialis ( 43 ), and C. gigas ( 25 , 44 – 46 ) have been described, but the complete access to information regarding any of the mollusk species remains unavailable. The Pacific oyster ( C. gigas ), a sessile filter feeder exposed to a wide range of biotic and abiotic stresses, is an attractive model to study the diversity of immune receptor families and the mediation of associated immune responses in invertebrates ( 47 , 48 ).…”

Section: Introductionmentioning

confidence: 99%

The Members of the Highly Diverse Crassostrea gigas Integrin Family Cooperate for the Generation of Various Immune Responses

Qiu

Wang

et al. 2020

Front. Immunol.

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Studies on invertebrate immune receptors can provide insights into characteristics specific to innate immune system. Here, eight α and three β integrins are identified from an invertebrate, the Pacific oyster Crassostrea gigas, and their possible immune functions are studied. Oyster α/β integrins exhibit a higher degree of sequence and structural variability than the members from Homo sapiens and Drosophila melanogaster. The analysis reveals that oyster RGD-and laminin-binding receptor homologs are present in the phylogenetic tree of α integrins, but the other six oyster α integrins mainly form a species-specific branch; meanwhile, oyster β integrins are clustered with insect β integrins but distinct from a member from the mollusk Biomphalaria glabrata. Although phylogenetically lacking the important α integrin branches of LDV-binding, PS3-type, and αI-containing integrins, oyster integrins can bind to most ECM ligands, including RGDCP, LDVCP, GFOGERCP, and laminin protein in a distinct binding pattern. Besides, oyster integrins are distributed in different hemocyte subpopulations, while only specific integrins are selectively involved in hemocyte phagocytosis, migration, and encapsulation, and some of them participate in more than one immune response in a sophisticated pattern. Especially, oyster β integrins are arranged in the core to mediate complex immune responses, unlike the counterparts in humans that mainly depend on αI-containing integrins to incite immune reactions. This study represents the first comprehensive attempt to reveal the structural and evolutionary features of the integrin family and their involvement in cellular immune responses in the non-model invertebrate C. gigas and sheds light on the characteristics specific to the innate immune system in the integrin family.

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“…E 2 also induced significant morphological changes in hemocytes, indicating activation, and stimulated oxidative burst, intracellular ROS production, lysozyme release and NO production (Canesi et al, 2004a(Canesi et al, , 2006b(Canesi et al, , 2007a. In the same experimental conditions, it was later shown that E 2 affected hemocyte adhesion to different substrates, and increased a2 integrin subunit levels (Koutsogiannaki and Kaloyianni, 2011). All the effects of E 2 occurred very rapidly, from 5 to 30-60 min post addition, and were mediated by non genomic mechanisms of action similar to those identified in mammalian cells; these involved increase of cytosolic Ca 2+ , activation of MAPKs (ERK, p38 and JNK) and PKC, and phosphorylation of the transcription factors STAT3, STAT5 and CREB (Canesi et al, 2004a(Canesi et al, , 2006b(Canesi et al, , 2007bPorte et al, 2006).…”

Section: Rapid Estrogen Signaling In Mytilus Hemocytesmentioning

confidence: 83%

“…However, the molluscan ER is an example of how the presence of homologous receptor sequences does not imply a homologous mechanism of action in vertebrate and invertebrate systems. Moreover, all components involved in membrane activated E 2 signaling (MAPKs, PI3K, PKC, Ca 2+ , GPRC, cAMP) (reviewed in Canesi et al 2007b;Fabbri and Capuzzo, 2010) as well as EGF-mediated pathways (Canesi et al, 2000) and integrin receptors (Koutsogiannaki and Kaloyianni, 2011;Maiorova and Odintsova, 2015) are present in mussel hemocytes. In this light, the involvement of putative GPER may further contribute to explain the rapid effects of E 2 in these cells.…”

Section: Transcriptomics Reveal Alternative Estrogen Signaling Pathwa...mentioning

confidence: 99%

Estrogenic compounds as exogenous modulators of physiological functions in molluscs: Signaling pathways and biological responses

Balbi

Ciacci

Canesi

2019

Comparative Biochemistry and Physiology Part C: Toxicology &

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Effect of 17β-estradiol on adhesion of Mytilus galloprovincialis hemocytes to selected substrates. Role of alpha2 integrin subunit

Cited by 11 publications

References 85 publications

Gill transcriptomic analysis in fast- and slow-growing individuals of Mytilus galloprovincialis

Gill transcriptomic analysis in fast- and slow-growing individuals of Mytilus galloprovincialis

The Members of the Highly Diverse Crassostrea gigas Integrin Family Cooperate for the Generation of Various Immune Responses

Estrogenic compounds as exogenous modulators of physiological functions in molluscs: Signaling pathways and biological responses

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