Efeitos da aplicação tópica do mel de Melipona subnitida em feridas infectadas de ratos

Summary Twenty‐four samples of Apis mellifera honey and twenty‐four samples of Melipona subnitida (Jandaira) honey were collected in the northeast of Brazil. Moisture, hydroxymethylfurfural, free acidity, insoluble solids in water, diastase activity, ashes, electrical conductivity, proteins, lipids, total carbohydrates, energy and sugars were the parameters analysed. The efficiency of the qualitative tests (Fiehe's test, Lugol's reaction, Lund's reaction) was tested. Pollen types and the corresponding plant species were identified in all samples (3 in Apis and 1 in Melipona). Apis mellifera honey samples demonstrated parameters in accordance with the Brazilian Legislation, while the Melipona subnitida honey samples displayed moisture (24.80%) and diastase activity (null) in discordance with the established by the regulation for Apis mellifera honeys. Apis honey samples presented higher values of electric conductivity (284.00 μS cm−1) than the obtained from the Jandaira honey samples (102.77 μS cm−1) as well as a darker colour (26.67 mmPfund) when compared with Jandaira honey (7.00 mmPfund). The concentration of the glucose, fructose and sucrose was higher in the Apis honeys than in the Jandaira honey. The characteristics of the two types of honey were very different, highlighting the need of developing specific legislation for stingless bees' honey.

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“…(), Alves et al . (), who have also found higher values of moisture content in stingless bees' honey. On the other hand, Almeida‐Muradian et al .…”

Section: Resultsmentioning

confidence: 83%

“…Similar values (0.01%) were obtained by Alves et al . (), who analysed stingless bees' honey. Concerning the Apis honey, the ash content was slightly inferior to the obtained by Estevinho et al .…”

Section: Resultsmentioning

confidence: 99%

Comparative study of the physicochemical and palynological characteristics of honey from Melipona subnitida and Apis mellifera

Almeida-Muradian

Stramm

Horita

et al. 2013

Int J of Food Sci Tech

113

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“…Until then, the methods used to evaluate the healing of these wounds evaluated at most only two dimensions (area) without assessing the depth [1][2][3][4][5][6][7][8][9][10][11][12] , which is the third dimension. The purpose of this study was to …”

Section: Discussionmentioning

confidence: 99%

A new proposal to evaluate the healing of open skin wounds: volumetry

Paulo

Fiorot

2011

Acta Cir. Bras.

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PURPOSE:To present a new proposal to evaluate the healing of an open subcutaneous and skin wound, which we termed "volumetry". METHODS:A total of 32 circular wounds were performed in the subcutaneous tissues and skin of four feet of pigs (8 each). Each wound had about 1 cm in diameter and was 0.2 cm deep. Volume was calculated from the wound filled with saline and mass Xantopren. With the aid of a magnifying glass and local lighting, the liquid was dripped with a micropipette inside the wound until complete fullness. Volume repletion was calculated in microliters, corresponding to the volume of the wound. The mass of Xantopren was placed inside the wound to obtain a mold of the lesion. Mold volume was calculated using the formula of the volume of a cylinder closest resembling a geometric figure with mold. RESULTS:The calculation of wound volume was possible with both Xantopren and mold techniques. Volume as calculated by micropipette was 119.37 ± 30.87 microliters while the volume calculated by mold was 122.41 ± 33.90 mm3 (p=0.79). CONCLUSIONS: CONCLUSÕES:A volumetria realizada em patas de suíno é um método simples, reprodutível. A volumetria realizada com líquido não diferiu da volumetria realizada com massa de Xantopren. Esse método poderá ser útil para auxiliar na análise da cicatrização da ferida cutânea aberta em trabalhos clínicos e experimentais.Descritores: Cicatrização. Pele. Suínos. -Acta Cirúrgica Brasileira -Vol. 26 (6) 2011Paulo DNS et al.

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“…14 A recent paper found that honey from Melipona subnitida Ducke, 1910 reduces the healing time of wounds, and was effective in eliminating Gram-negative bacteria such as Pseudomonas sp., Klebsiella sp., Escherichia coli, and Acinetobacter sp. 15 Melipona marginata Lepeletier, 1836 is commonly known as ''manduri, manduri menor, mandurim, minduri, gurupu-do-miúdo, and taipeira'' in Brazil. This stingless bee is an endangered species that can be found in Northeast, Southeast and South Brazil as well as in Argentina and Paraguay.…”

Section: Introductionmentioning

confidence: 99%

Topical Anti-Inflammatory Activity of a Monofloral Honey of Mimosa scabrella Provided by Melipona marginata During Winter in Southern Brazil

Borsato

Prudente²,

Döll-Boscardin³

et al. 2014

Journal of Medicinal Food

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Melipona marginata is an endangered species of stingless bee from Brazil that produces honey with particular physicochemical features and a remarkable exotic flavor. To the best of our knowledge, this is the first report devoted to exploring the medicinal potential of this honey. Thus, the aim of this paper was to investigate the potential anti-inflammatory activity of honey extract from M. marginata on skin inflammation. The honey sample was classified as a monofloral honey of Mimosa scabrella. The presence of 11 phenolic compounds as kaempferol and caffeic acid was detected using the high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-UV-ESI-MS) method. The anti-inflammatory activity was measured using a 12-O-tetradecanoylphorbol-13-acetate-induced ear edema model of inflammation in mice. The topical application of the M. marginata honey extract (1.0 mg/ear) was able to reduce ear edema with an inhibitory effect of 54 ± 5%. This extract decreased the myeloperoxidase activity in 75 ± 3%, which suggests a lower leucocyte infiltration that was confirmed by histological analysis. This extract also provided a reduction of 55 ± 14% in the production of reactive oxygen species. This anti-inflammatory activity could be due to a synergic effect of the phenolic compounds identified in the honey sample. Taken together, these results open up new possibilities for the use of M. marginata honey extract in skin disorders.

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Efeitos da aplicação tópica do mel de Melipona subnitida em feridas infectadas de ratos

Cited by 18 publications

References 16 publications

Comparative study of the physicochemical and palynological characteristics of honey from Melipona subnitida and Apis mellifera

Comparative study of the physicochemical and palynological characteristics of honey from Melipona subnitida and Apis mellifera

A new proposal to evaluate the healing of open skin wounds: volumetry

Topical Anti-Inflammatory Activity of a Monofloral Honey of Mimosa scabrella Provided by Melipona marginata During Winter in Southern Brazil

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