Efeito protetor do per-condicionamento isquêmico remoto nas lesões da síndrome de isquemia e reperfusão renal em ratos

Abstract

Protective effect of remote ischemic per-conditioning in the Protective effect of remote ischemic per-conditioning in the Protective effect of remote ischemic per-conditioning in the Protective effect of remote ischemic per-conditioning in the Protective effect of remote ischemic per-conditioning in the ischemia and reperfusion-induce renal injury in rats ischemia and reperfusion-induce renal injury in rats ischemia and reperfusion-induce renal injury in rats ischemia and reperfusion-induce renal injury in rats ischemia and reperfusion-induce renal injury in rats Efeito protetor do per-condicionamento isquêmico remoto nas lesões da Efeito protetor do per-condicionamento isquêmico remoto nas lesões da Efeito protetor do per-condicionamento isquêmico remoto nas lesões da Efeito protetor do per-condicionamento isquêmico remoto nas lesões da Efeito protetor do per-condicionamento isquêmico remoto nas lesões da síndrome de isquemia e reperfusão renal em ratos síndrome de isquemia e reperfusão renal em ratos síndrome de isquemia e reperfusão renal em ratos síndrome de isquemia e reperfusão renal em ratos síndrome de isquemia e reperfusão renal em ratos Fifteen rats (Rattus norvegicus) were randomized into three groups (n = 5): Group Normality (GN), ControlIschemia and Reperfusion (GIR) and Group remote ischemic per-conditioning (GPER). With the exception of the GN group, all others underwent renal ischemia for 30 minutes. In group GPER we performed the ischemic remote per-conditioning, consisting of three cycles of ischemia and reperfusion applied every five minutes during the ischemic period, to the left hindlimb of the rats by means of a tourniquet. To quantify the lesions we measured serum levels of creatinine and urea, as well as analyzed renal histopathology. Results Results ResultsResults Results: The GPER group presented with better levels of urea (83.74 ± 14.58%) and creatinine (0.72 ± 26.14%) when compared to GIR group, approaching the GN group. Histopathologically, the lower levels of medullary congestion and hydropic degeneration were found in group GPER. Conclusion Conclusion Conclusion Conclusion Conclusion: The remote ischemic per-conditioning had a significant protective effect on renal ischemia and reperfusion.

Perconditioning combined with postconditioning on kidney ischemia and reperfusion

“…The vascular anatomy of kidney is unique 22,30 where glomerulus receive blood supply prior to medullar area (tubulus), being very different from the heart or liver vascular anatomy; myocites have also different cell biology. Our findings that PER might confer protection to the glomerulus and tubules and POS only to the glomerulus, support that tissue conditioning techniques might work in a tissue dependent manner.…”

“…We tested three cycles of 5 min of reperfusion followed by 5 min ischemia, interval referred to previous literature 18,22,32 . Whether, the ischemic per and postconditioning plays its role in an 'on-off' style or a 'dose-dependent' one 22 …”

“…Whether, the ischemic per and postconditioning plays its role in an 'on-off' style or a 'dose-dependent' one 22 …”

“…Renal ischemia was induced by applying a microsurgical clip on the renal artery. Hindlimb ischemia was achieved using an elastic rubber band tied around the thigh of the left leg, through a non-invasive method that was successfully used in previous studies 18,22 .…”

“…PER might be even more practical than POS, because it is performed in a distant tissue and is a non-invasive procedure, thus does not require direct access to the artery and could be easily applied during endovascular revascularization 18,22 . Mechanisms underlying the remote ischemic conditioning are barely understood.…”