-The overseeding of ryegrass (Lolium multiflorum Lam) on sub-tropical native pastures increases the availability and quality of forage in the cold season. However, soil chemical amendment and nitrogen use can affect the floristic composition, with consequences to the primary and secondary production. In a randomized complete block design with three replications, the effect of nitrogen fertilization (40, 90 and 140 kg/ha of nitrogen) on native pasture overseeded with ryegrass in two consecutive years was assessed regarding production and composition of pasture and performance of beef calves. In both years, the forage accumulation rate and forage production responded linearly to the increase in nitrogen rates as a result The production and composition of forage and livestock production are improved by the use of nitrogen, but the efficiency of responses can be affected by sowing date and nitrogen fertilization.