Editorial: Silkworm Genome

Couble, Pierre; Mita, Kasuei; Xia, Qingyou

doi:10.1016/j.ibmb.2009.01.004

Cited by 4 publications

(2 citation statements)

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“…S1). It was located on chromosome 25 in silkworm precise mapping [37], which includes a single copy of a completed 705 bp coding frame, and consists of four exons spanning a 2.9 kb fragment in nscaf2823 (Fig. 1A).…”

Section: Resultsmentioning

confidence: 99%

Basic Helix-Loop-Helix Transcription Factor Bmsage Is Involved in Regulation of fibroin H-chain Gene via Interaction with SGF1 in Bombyx mori

Zhao

Lei

et al. 2014

PLoS ONE

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Silk glands are specialized in the synthesis of several secretory proteins. Expression of genes encoding the silk proteins in Bombyx mori silk glands with strict territorial and developmental specificities is regulated by many transcription factors. In this study, we have characterized B. mori sage, which is closely related to sage in the fruitfly Drosophila melanogaster. It is termed Bmsage; it encodes transcription factor Bmsage, which belongs to the Mesp subfamily, containing a basic helix–loop–helix motif. Bmsage transcripts were detected specifically in the silk glands of B. mori larvae through RT-PCR analysis. Immunoblotting analysis confirmed the Bmsage protein existed exclusively in B. mori middle and posterior silk gland cells. Bmsage has a low level of expression in the 4th instar molting stages, which increases gradually in the 5th instar feeding stages and then declines from the wandering to the pupation stages. Quantitative PCR analysis suggested the expression level of Bmsage in a high silk strain was higher compared to a lower silk strain on day 3 of the larval 5th instar. Furthermore, far western blotting and co-immunoprecipitation assays showed the Bmsage protein interacted with the fork head transcription factor silk gland factor 1 (SGF1). An electrophoretic mobility shift assay showed the complex of Bmsage and SGF1 proteins bound to the A and B elements in the promoter of fibroin H-chain gene(fib-H), respectively. Luciferase reporter gene assays confirmed the complex of Bmsage and SGF1 proteins increased the expression of fib-H. Together, these results suggest Bmsage is involved in the regulation of the expression of fib-H by being together with SGF1 in B. mori PSG cells.

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Section: Resultsmentioning

confidence: 99%

Basic Helix-Loop-Helix Transcription Factor Bmsage Is Involved in Regulation of fibroin H-chain Gene via Interaction with SGF1 in Bombyx mori

Zhao

Lei

et al. 2014

PLoS ONE

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show abstract

“…This assembly revealed that the B. mori genome contains chromosomal remnants of an ancestral karyotype that existed before the divergence of vertebrates and insects, which was not as clear in other insect genomes (Couble et al, 2008). Therefore, the B. mori genome project offers an interesting opportunity to systematically survey its GPCRs, in an attempt to facilitate relevant research, such as characterizing B. mori GPCRs, understanding the regulation of GPCRs throughout Lepidopteran development, and illustrating the evolution and adaptation of insects.…”

Section: Introductionmentioning

confidence: 99%