PLoS ONE volume 9, issue 5, Pe98360 2014 DOI: 10.1371/journal.pone.0098360 View full text
Stephanie Trauth, Ilka B. Bischofs

Abstract: Fluorescent protein promoter reporters are important tools that are widely used for diverse purposes in microbiology, systems biology and synthetic biology and considerable engineering efforts are still geared at improving the sensitivity of the reporter systems. Here we focus on dark noise, i.e. the signal that is generated by the empty vector control. We quantitatively characterize the dark noise of a few common bacterial reporter systems by single cell microscopy. All benchmarked reporter systems generated …

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