Ectopic expression of LLAG1, an AGAMOUS homologue from lily (Lilium longiflorum Thunb.) causes floral homeotic modifications in Arabidopsis

Benedito, Vagner A.; Visser, Peter B.; Tuyl, J.M. van; Angenent, Gerco C.; Vries, S.C. de; Krens, Frans A.

doi:10.1093/jxb/erh156

Cited by 47 publications

(39 citation statements)

References 49 publications

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“…It included a non-functional N terminal extension of the MADS-box since the truncated AG without this N terminal extension functioned normally in vitro (Huang et al 1993;Pollock and Treisman 1991). However, not all AG homolog genes have this N terminal extension (Benedito et al 2004;Kang et al 1995;Li et al 2002). Non-function of this extension might be the reason for its loss during evolution (Benedito et al 2004).…”

Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of an AGAMOUS Homolog from Fraxinus pennsylvanica

Pijut

2010

Plant Mol Biol Rep

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An AGAMOUS homolog (FpAG) was isolated from green ash (Fraxinus pennsylvanica) using a reverse transcriptase polymerase chain reaction method. Southern blot analysis indicated that FpAG was present as a singlecopy sequence in the genome of green ash. RNA accumulated in the reproductive tissues (female inflorescence, male inflorescence, and fruit) and vegetative tissues (leaves and in vitro-germinated seedlings). Expression was higher in reproductive tissues than in vegetative tissues. Ectopic expression of FpAG in transgenic Arabidopsis plants resulted in a range of weak to strong APETALA2 (AP2) mutant-like phenotypes, including early flowering, curly leaves, and conversion of petals to stamens. These data indicate functional homology between FpAG and AGAMOUS.

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Section: Discussionmentioning

confidence: 99%

Isolation and Characterization of an AGAMOUS Homolog from Fraxinus pennsylvanica

Pijut

2010

Plant Mol Biol Rep

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“…LLAG1 and LMADS10 were expressed in stamens and carpels, whereas LMADS2 mRNA accumulated primarily in ovules and weakly in style tissue of the carpel (Benedito et al, 2004;Hsu et al, 2010;Tzeng et al, 2002). Ectopic expression of LLAG1 and LMADS10 in A. thaliana affected the outer two whorls; sepals were converted into carpel-like structures in whorl 1, and petals were sometimes converted into stamen-like structures in whorl 2 in transformants expressing 35S::LLAG1 (Benedito et al, 2004), whereas these were occasionally absent in transformants expressing 35S::LMADS10 (Hsu et al, 2010). The conversion of sepals into carpelloid structures and petals into staminoid structures occurred in A. thaliana transformants overexpressing LMADS2 (Tzeng et al, 2002); therefore, lily D-lineage genes may have stronger effects than C-lineage genes in A. thaliana (Hsu et al, 2010).…”

Section: -2) Lily-type Flowers-a Perianth With Two Petaloid Whorlsmentioning

confidence: 97%

Section: -2) Lily-type Flowers-a Perianth With Two Petaloid Whorlsmentioning

confidence: 99%

“…Two distinct C-lineage genes, LLAG1 and LMADS10, and one D-lineage gene, LMADS2, have been isolated (Benedito et al, 2004;Hsu et al, 2010;Tzeng et al, 2002). LLAG1 and LMADS10 were expressed in stamens and carpels, whereas LMADS2 mRNA accumulated primarily in ovules and weakly in style tissue of the carpel (Benedito et al, 2004;Hsu et al, 2010;Tzeng et al, 2002). Ectopic expression of LLAG1 and LMADS10 in A. thaliana affected the outer two whorls; sepals were converted into carpel-like structures in whorl 1, and petals were sometimes converted into stamen-like structures in whorl 2 in transformants expressing 35S::LLAG1 (Benedito et al, 2004), whereas these were occasionally absent in transformants expressing 35S::LMADS10 (Hsu et al, 2010).…”

Section: -2) Lily-type Flowers-a Perianth With Two Petaloid Whorlsmentioning

confidence: 99%

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Molecular Mechanism Regulating Floral Architecture in Monocotyledonous Ornamental Plants

Kanno

2016

The Hortic J

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Molecular and genetic analyses of flower development have been conducted primarily in dicot model plants such as Arabidopsis thaliana and Antirrhinum majus. The obtained data are the basis for the ABC model, which was extended to the ABCE model of floral development. This model has been validated in many dicot species using genetic transformation studies and mutant analyses. Many dicot flowers have two distinctive perianth whorls, which include greenish sepals and showy petals. By contrast, the monocot lily flower has two almost identical petaloid whorls, the inner and outer tepals. To explain this type of floral morphology, a modified ABC model, the further extended modified ABCE model, was proposed. According to this model, B-class genes are expressed in whorls 1-3, and whorls 1 and 2 form petaloid structures. This review describes the molecular mechanisms regulating flower development in monocots. Since the showy perianth is one of the most important traits in floricultural crops, we focused on the B-and C-class genes, which are related to the development and modification of the perianth. The review describes the expression patterns of floral organ identity genes, and presents functional studies using double-flowered and viridiflora cultivars in some monocot species. Besides lily-type flowers, there are several types of monocot flower, such as commelina type with two whorls of distinctive perianth, orchid and grass flowers. The review also describes the molecular analyses of these types of monocot flower.

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“…In monocots, AG-like genes have been studied in rice (Kang et al 1995(Kang et al , 1998, maize (Mena et al 1996), lily (Tzeng et al 2002;Benedito et al 2004), Hyacinthus (Li et al 2002;Xu et al 2004), and Asparagus (Yun et al 2004a,b). While phylogenetic positions of D-lineage AG-like genes from grass plants are unclear because the phylogenetic positions of these genes in NJ tree does not coincide in those of 4).…”

Section: Phylogenetic Implications Of the MD Motif In Monocotsmentioning

confidence: 99%

Spatiotemporal expression of duplicate AGAMOUS orthologues during floral development in Phalaenopsis

et al. 2006

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The AGAMOUS (AG) family of MADS-box genes plays important roles in controlling the development of the reproductive organs of flowering plants. To understand the molecular mechanisms behind the floral development in the orchid, we isolated and characterized two AG-like genes from Phalaenopsis that we denoted PhalAG1 and PhalAG2. Phylogenetic analysis indicated that PhalAG1 and PhalAG2 fall into different phylogenetic positions in the AG gene family as they belong to the C- and D-lineages, respectively. Reverse transcription-polymerase chair reaction (RT-PCR) analyses showed that PhalAG1 and PhalAG2 transcripts were detected in flower buds but not in vegetative organs. Moreover, in situ hybridization experiments revealed that PhalAG1 and PhalAG2 hybridization signals were observed in the lip, column, and ovule during the floral development of Phalaenopsis, with little difference between the expression patterns of the two genes. These results suggest that both AG-like genes in Phalaenopsis act redundantly with each other in floral development.

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Ectopic expression of LLAG1, an AGAMOUS homologue from lily (Lilium longiflorum Thunb.) causes floral homeotic modifications in Arabidopsis

Cited by 47 publications

References 49 publications

Isolation and Characterization of an AGAMOUS Homolog from Fraxinus pennsylvanica

Isolation and Characterization of an AGAMOUS Homolog from Fraxinus pennsylvanica

Molecular Mechanism Regulating Floral Architecture in Monocotyledonous Ornamental Plants

Spatiotemporal expression of duplicate AGAMOUS orthologues during floral development in Phalaenopsis

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