Ectopic Expression of Capsicum-Specific Cell Wall Protein Capsicum annuum Senescence-Delaying 1 (CaSD1) Delays Senescence and Induces Trichome Formation in Nicotiana benthamiana

Abstract

Secreted proteins are known to have multiple roles in plant development, metabolism, and stress response. In a previous study to understand the roles of secreted proteins, Capsicum annuum secreted proteins (CaS) were isolated by yeast secretion trap. Among the secreted proteins, we further characterized Capsicum annuum senescence-delaying 1 (CaSD1), a gene encoding a novel secreted protein that is present only in the genus Capsicum. The deduced CaSD1 contains multiple repeats of the amino acid sequence KPPIHNHKPTDYDRS. Interestingly, the number of repeats varied among cultivars and species in the Capsicum genus. CaSD1 is constitutively expressed in roots, and Agrobacterium-mediated transient overexpression of CaSD1 in Nicotiana benthamiana leaves resulted in delayed senescence with a dramatically increased number of trichomes and enlarged epidermal cells. Furthermore, senescence-and cell division-related genes were differentially regulated by CaSD1-overexpressing plants. These observations imply that the pepper-specific cell wall protein CaSD1 plays roles in plant growth and development by regulating cell division and differentiation.

SNP Marker Integration and QTL Analysis of 12 Agronomic and Morphological Traits in F8RILs of Pepper (Capsicum annuum L.)

“…Moreover, medical usage of pepper is also developed to alleviate the pain and long-term inflammation (Ben Chaim et al, 2006). Therefore, it has been attracting attention of breeders and geneticists (Hwang et al, 2009;Kwon and Kim, 2009;Seo et al, 2012).…”

OsWRKY42 Represses OsMT1d and Induces Reactive Oxygen Species and Leaf Senescence in Rice

“…Overnight cultures were harvested and resuspended in 10 mM MgCl 2 to an OD 600 of 0.5. After incubation at room temperature for 3 h, the cells were used to infiltrate six-week-old káÅçíá~å~= ÄÉåíÜ~ãá~å~ leaves using a needleless syringe (1 ml) as described previously (Romeis et al, 2001;Seo et al, 2012). Plants were grown and maintained throughout the experiments in a growth chamber at a temperature of 25°C and under 12 h of light.…”

“…2) (Romeis et al, 2001;Seo et al, 2012). At 10 days after inoculation, only lë= tohvQOJÅjóÅ expression (OsW42) caused early leaf senescence compared to the controls, ^ÖêçÄ~ÅíÉêáìã= background GV3101 strain and ^ÖêçÄ~ÅíÉêáìã carrying empty vector (EV) or PRpWdcm (GFP).…”

Identification of CaLOP Regulating Development and Growth Through Virus-Induced Gene Silencing in Pepper

“…식물의 다양한 방어기제에 중요한 역할을 하는 것으로 알려 진 분비단백질은 식물이 병원균에 공격을 받았을 때 세포 편광과 유두 형성을 통한 세포벽 비대에 관여하는 것으로 보고되었다 (Schulze-Lefert, 2004). 또한, PR 단백질(pathogenesis-related protein)과 같은 분비단백질은 systemic acquired resistance (SAR)을 통한 식물의 저항성에 기여하는 것으로 보고되었고 (Schmelzer, 2002;Yeom et al, 2012), 식물의 형태 변화, 조직 분 화 및 생장과 같은 생육과 발달에도 중요한 역할을 한다고 알려져 있다 (Yamane et al, 2005;Lee et al, 2011;Yeom et al, 2011;Seo et al, 2012).…”

“…고추 역병균에 대해 저항성 고추와 감수성 고추에서 특이적으로 발현되는 분비단 백질을 코딩하는 유전자들에 대한 발현 분석을 통해 후보 유전자들을 선별하고, 이들에 대해 바이러스 기반 유전자 침묵 기법 (virus-induced gene silencing, VIGS)을 통해 저항성 기능 분석을 수행하였다. 또한, 병원균과 상호작용하는 CaS 유전자가 식 물의 생육과 발달에도 중요한 역할을 할 것이라는 가능성도 제시하였다 (Seo et al, 2012). 하지만, 그 유전자들의 다양한 환경 스트레스에 대한 상호작용과 식물의 생육 및 발달에 관한 생물학적 기능에 대해서는 제한적으로 연구가 진행되었다.…”