a b s t r a c tEcosystem services evaluation aims at understanding the status of ecosystem services on different spatial and temporal scale. In this paper, we selected the middle reach of the Heihe River Basin (HRB), which is the second largest inland river basin in China, as one of the typical area to estimate the ecosystem services values (ESVs) corresponding to the land use changes. Based on the land use data and ecosystem service value coefficients, the total ecosystem services values (TESVs) of the middle reach of the HBR are quantitatively calculated, which were 9.244 Â 108, 9.099 Â 108, 9.131 Â 108 and 9.146 Â 108 USD in 1988, 2000 respectively. During 1988, the decrease of grassland, forest land, water area and unused land contributed 148.94%, 57.85%, 87.87% and 16.42% respectively to the net loss of TESVs, while the dramatic increase of cultivated land improved the TESVs with contribution of À211.08% to the net loss of TESVs. Expansion of cultivated land, which especially caused the loss of grassland and forest land, directly exerted negative impacts on the provision of ecosystem services in the study area. The findings of this research indicated that land use change was an important form of human activities, which had a strong impact on ecosystem services.