Ecdysteroid activity during metamorphosis and in male and female adults of four blowfly species

Accessory Gland as a Site for Prothoracicotropic Hormone Controlled Ecdysone Synthesis in Adult Male Insects

“…Ecdysteroid levels in adult males are generally lower compared to the high-level pulses produced by the PG that drive transitions during development and those of female insects [22], [39]. This is consistent with our finding that Halloween gene expression in male adults is low compared to larvae, pupae and females.…”

Accessory Gland as a Site for Prothoracicotropic Hormone Controlled Ecdysone Synthesis in Adult Male Insects

“…Ecdysteroid levels in adult males are generally lower compared to the high-level pulses produced by the PG that drive transitions during development and those of female insects [22], [39]. This is consistent with our finding that Halloween gene expression in male adults is low compared to larvae, pupae and females.…”

Ecdysteroids: the overlooked sex steroids of insects? Males: the black box

“…This clearly indicated that (adult) males do not synthesize 20E. This is in agreement with the RIA data of Briers et al (1983) in several flies: ecdysteroid titres in males were almost undetectable.…”

“…To my knowledge, the antibody prepared and used by Briers and De Loof (1981a, b) has been the only one that was very specific for 20E. Ecdysteroid titre measurements by RIA with this antibody in four fly species, namely Sarcophaga (Neobellieria) bullata, Lucilia caesar, Phormia terraenovae, and Calliphora vicina showed that females have titres ranging from 7-15 ng/mL while in males the values were always below the detection limit of 1 ng/mL (Briers et al, 1983). Injection of 20E into males of all these species as well as Drosophila, induced vitellogenin synthesis (Huybrechts & De Loof, 1977, 1982Bownes et al, 1982).…”

Transcriptomic Evidence for Cell-Autonomous Sex Differentiation of the Gynandromorphic Fat Body in the Silkworm, Bombyx mori

“…This study was performed to quantify the integrity of secondary sexual trait development in gynandromorphic silkworms to clarify the contributions of cell-autonomous and non-cell-autonomous regulation. As a secondary sexual trait, we focused on the fat body because it shows remarkable sexual dimorphism in physiological properties represented by the biosynthesis of plasma proteins and proteins involved in oogenesis regulated by both cell-autonomous and non-cell-autonomous mechanisms [ 37 , 56 , 58 , 59 , 60 , 62 , 63 ].…”

Accessory Gland as a Site for Prothoracicotropic Hormone Controlled Ecdysone Synthesis in Adult Male Insects

“…Ecdysteroid levels in adult males are generally lower compared to the high-level pulses produced by the PG that drive transitions during development and those of female insects [22], [39]. This is consistent with our finding that Halloween gene expression in male adults is low compared to larvae, pupae and females.…”

Ecdysteroids: the overlooked sex steroids of insects? Males: the black box

“…This clearly indicated that (adult) males do not synthesize 20E. This is in agreement with the RIA data of Briers et al (1983) in several flies: ecdysteroid titres in males were almost undetectable.…”

“…To my knowledge, the antibody prepared and used by Briers and De Loof (1981a, b) has been the only one that was very specific for 20E. Ecdysteroid titre measurements by RIA with this antibody in four fly species, namely Sarcophaga (Neobellieria) bullata, Lucilia caesar, Phormia terraenovae, and Calliphora vicina showed that females have titres ranging from 7-15 ng/mL while in males the values were always below the detection limit of 1 ng/mL (Briers et al, 1983). Injection of 20E into males of all these species as well as Drosophila, induced vitellogenin synthesis (Huybrechts & De Loof, 1977, 1982Bownes et al, 1982).…”

Transcriptomic Evidence for Cell-Autonomous Sex Differentiation of the Gynandromorphic Fat Body in the Silkworm, Bombyx mori

“…This study was performed to quantify the integrity of secondary sexual trait development in gynandromorphic silkworms to clarify the contributions of cell-autonomous and non-cell-autonomous regulation. As a secondary sexual trait, we focused on the fat body because it shows remarkable sexual dimorphism in physiological properties represented by the biosynthesis of plasma proteins and proteins involved in oogenesis regulated by both cell-autonomous and non-cell-autonomous mechanisms [ 37 , 56 , 58 , 59 , 60 , 62 , 63 ].…”

Accessory Gland as a Site for Prothoracicotropic Hormone Controlled Ecdysone Synthesis in Adult Male Insects

“…Ecdysteroid levels in adult males are generally lower compared to the high-level pulses produced by the PG that drive transitions during development and those of female insects [22], [39]. This is consistent with our finding that Halloween gene expression in male adults is low compared to larvae, pupae and females.…”

Ecdysteroids: the overlooked sex steroids of insects? Males: the black box

“…This clearly indicated that (adult) males do not synthesize 20E. This is in agreement with the RIA data of Briers et al (1983) in several flies: ecdysteroid titres in males were almost undetectable.…”

“…To my knowledge, the antibody prepared and used by Briers and De Loof (1981a, b) has been the only one that was very specific for 20E. Ecdysteroid titre measurements by RIA with this antibody in four fly species, namely Sarcophaga (Neobellieria) bullata, Lucilia caesar, Phormia terraenovae, and Calliphora vicina showed that females have titres ranging from 7-15 ng/mL while in males the values were always below the detection limit of 1 ng/mL (Briers et al, 1983). Injection of 20E into males of all these species as well as Drosophila, induced vitellogenin synthesis (Huybrechts & De Loof, 1977, 1982Bownes et al, 1982).…”

Transcriptomic Evidence for Cell-Autonomous Sex Differentiation of the Gynandromorphic Fat Body in the Silkworm, Bombyx mori

“…This study was performed to quantify the integrity of secondary sexual trait development in gynandromorphic silkworms to clarify the contributions of cell-autonomous and non-cell-autonomous regulation. As a secondary sexual trait, we focused on the fat body because it shows remarkable sexual dimorphism in physiological properties represented by the biosynthesis of plasma proteins and proteins involved in oogenesis regulated by both cell-autonomous and non-cell-autonomous mechanisms [ 37 , 56 , 58 , 59 , 60 , 62 , 63 ].…”