Early changes in auxin and ethylene production in vine cuttings before adventitious rooting

Moncousin, Charles; Favre, Jean-Michel; Gaspar, Thomas

doi:10.1007/bf00043350

Cited by 26 publications

(8 citation statements)

References 11 publications

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“…Although ethylene at low levels of exogenous applications (0.01-1 ppm) did not influence rooting of petunia microshoots in an auxin-free medium, it induced adventitious root formation considerably at a concentration of 10 ppm without any effect on the root length. The contradictory experimental results in the literature (Krishnamoorthy 1970(Krishnamoorthy , 1972Roy et al 1972;Coleman et al 1980;Robbins et al 1983;Geneve et al 1990) support the hypothesis that the influence of exogenous ethylene on adventitious root formation varies with the plant species and the physiological and environmental conditions (Moncousin et al 1989;Mudge 1989).…”

Section: Discussionmentioning

confidence: 66%

Promotion of petunia (Petunia hybrida L.) regeneration in vitro by ethylene

Dimasi-Theriou

Economou

Sfakiotakis

1993

Plant Cell Tiss Organ Cult

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The influence of ethylene on shoot and root formation from petunia leaf explants was studied in cultures in test tubes placed in 5 1 glass jars. Reduction of the endogenously produced ethylene by inclusion of ethysorb (KMnO4) , an ethylene absorbent, caused a decrease of the number of shoots. On the other hand, supplementing the cultures with ethylene (0.01-10 ppm) caused a marked increase of the number of shoots without, however, any effect on the length and fresh weight. Ethylene treatments (1 ppm) were found to be most effective when they were applied in the second week of culturing of petunia explants. Addition of Co ++ to the medium resulted in a reduction of the endogenously produced ethylene and concomitantly reduced shoot formation. Similarly, inclusion of Ag +, an inhibitor of ethylene action, resulted in poor shoot formation. Ethylene also appeared to play a role on rooting of petunia microshoots in vitro in an auxin-free medium. Ethylene at a concentration of 10 ppm induced adventitious root formation considerably, whereas at low levels (0.01-1ppm) it had no influence on rooting.

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Section: Discussionmentioning

confidence: 66%

Promotion of petunia (Petunia hybrida L.) regeneration in vitro by ethylene

Dimasi-Theriou

Economou

Sfakiotakis

1993

Plant Cell Tiss Organ Cult

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“…Several conditions have been created by which the rooting performance decreases. Sometimes the expected reduction of the peroxidase peak occurs (Moncousin and Ducreux, 1984) (Moncousin et al, 1989;Hausman, 1993) and polyamines (Torrigiani et al, 1989;Hausman et al, 1994) …”

Section: Biochemical Markersmentioning

confidence: 99%

Markers of adventitious root formation

Klerk¹

1996

Agronomie

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“…5). MONCOUSIN et al (1989) discovered that during the rooting of nodal segments of vine cuttings on a medium without growth regulators, the production of ethylene increased 2 hours after the establishment of the experiment (the values correspond to stress), then after 10 hours again, with a parallel increase in endogenous auxin, and before bud flushing again. FIŠEROVÁ and HRADILÍK (1994) also reported increased production of ethylene before the flushing of apical vine cuttings.…”

Section: Resultsmentioning

confidence: 99%

The effect of growth regulators on the rooting of shoots of the peach rootstock Ishtara in in vitro conditions

Aw¹,

Křižan²,

Klemš³

et al. 2004

Hort. Sci. (Prague)

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The cloning of fruit tree species in vitro is a process that is introduced all over the world (FARI et al. 1996) with the aim to obtain virus-free and identical plant material. The selection of peach rootstocks (Prunus persica, P. domestica, P. armeniaca, P. amygdalus and their crosses) is based on the soil properties, calcium content in the soil and the incidence of nematodes (Meloidogyne arenaria, M. incognita, M. javanica). The Ishtara rootstock tolerates humid soil and is nematode-resistant, the reason why the propagation of this rootstock by means of the explant technique in conditions in vitro is very promising. As Ishtara is a rootstock with a high propagation capacity, but different rooting (NÉMETH 1981;JONES, HOOPGOOD 1979; DRUART 1980;KARHU, ULVINEN 1995), the process of rhizogenesis was studied under in vitro conditions.In 1912, on the basis of his morphological experiments, DOSTÁL reported that specific regulating effects arising from the growing axillary buds (the effect of auxins) boost the formation of adventitious roots, and in 1934 THIMANN and WENT announced that lexogenously applied IBA stimulated the formation of adventitious roots.Many authors who studied the correlation between rhizogenesis and ethylene frequently achieved different results. As early as in 1933 ZIMMERMANN and HITCH-COCK proved the stimulating effect of ethylene on the formation of adventitious roots; RADIN and LOOMIS (1969) discovered the inhibiting effect of ethylene (2 to 3 μl/l) on the formation of lateral roots in radish; and DIMASI-THERIOU et al. (1993) reported that a concentration of 0.01-10 μl/l of ethylene increased the number of shoots and adventitious roots in petunia leaf regeneration.A frequent problem of in vitro cultivation in pots is not only the high concentration of ethylene and ethane released by damaged cells that is considered as the indicator of lipid peroxidase (CELIKEL, VANDOORN 1995) but also the CO 2 concentration. BALLA et al. (2003) examined the process of rooting influenced either by an addition of saccharose into the medium or application of CO 2 into the cultivation area (3-4%); they discovered that an increased level of atmospheric CO 2 in parallel with a reduced level of saccharose in the medium resulted in the same % of rooting of cuttings of apple and prunus as if the medium contained 60 g/l of saccharose.According to GRANT et al. (1992) a higher content of CO 2 stimulated the closing of stomata, after which transpiration in the plants decreased, increasing the water potential of the cuttings; at the same time the starch level in the plants increased, apparently due to a higher partial pressure created by the applied CO 2 . Although the increased starch concentration increased the dry matter content in Correa schlechtendalii (Behr) by 70-90%, in Chamelaucia this increase was only 10-30% and that is why the authors incline to believe that increased rhizogenesis is due to the improved water conditions in the plant.It is not clear yet whether abscisic acid generated in the roots and with con...

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Early changes in auxin and ethylene production in vine cuttings before adventitious rooting

Cited by 26 publications

References 11 publications

Promotion of petunia (Petunia hybrida L.) regeneration in vitro by ethylene

Promotion of petunia (Petunia hybrida L.) regeneration in vitro by ethylene

Markers of adventitious root formation

The effect of growth regulators on the rooting of shoots of the peach rootstock Ishtara in in vitro conditions

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