Early biological responses in ocular tissue after SMILE and LASIK surgery

Khamar, Pooja; Nishtala, Krishnatej; Shetty, Rohit; Panigrahi, Trailokyanath; Shetty, K Bhujang; Pahuja, Natasha; Deshpande, Vrushali; Ghosh, Arkasubhra

doi:10.1016/j.exer.2020.107936

Cited by 10 publications

(9 citation statements)

References 47 publications

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“…We observed higher expressions of cathepsin B, cofilin and profilin 1 in fibrotic tissue, which validated our observations from the in vitro model. The recently reported proteomic profile of human corneal stroma by Khamar et al [ 20 ] has an overlap of 111 proteins with the in vitro primary stromal cell differentiation model ( Figure 6 A). The list of 111 proteins is shown in Figure 6 B.…”

Section: Discussionmentioning

confidence: 93%

“…MMP-2, -9 and -3 have all been connected to keratocyte migration and fibroblast activation at the wound site, in addition to the construction of the basement membrane and stromal remodeling [ 19 ]. Recently, it has been shown that epithelium-derived factors may predispose certain subjects to develop corneal haze post−refractive surgery through PREX1, which also regulates the TGF-β pathway [ 20 ]. Therefore, regulating TGF-β-dependent pathways for modulation of wound healing and preventing visual loss due to scarring is attractive.…”

Section: Introductionmentioning

confidence: 99%

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Quantitative Proteomics Reveals Molecular Network Driving Stromal Cell Differentiation: Implications for Corneal Wound Healing

Nishtala

Panigrahi

Shetty

et al. 2022

IJMS

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The differentiation of keratocytes to fibroblasts and myofibroblasts is an essential requisite during corneal wound closure. The aim of this study is to uncover factors involved in differentiation-dependent alteration in the protein profile of human corneal stromal cells using quantitative proteomics. Human corneal fibroblasts were cultured and differentiated into keratocytes in serum-free media and myofibroblasts through treatment with TGF-β. The protein cell lysates from the donors were tryptic and were digested and labeled using a 3-plex iTRAQ kit. The labeled peptides were subjected to LCMS analysis. Biological functional analysis revealed a set of crucial proteins involved in the differentiation of human corneal stromal cells which were found to be significantly enriched. The selected proteins were further validated by immunohistochemistry. Quantitative proteomics identified key differentially expressed proteins which are involved in cellular signaling pathways. Proteins involved in integrin signaling (Ras-RAP1b, TLN and FN) and SLIT-ROBO pathways (PFN1, CAPR1, PSMA5) as well as extracellular matrix proteins (SERPINH1, SPARC, ITGβ1, CRTAP) showed enhanced expression in corneal fibroblasts and myofibroblasts compared to keratocytes, indicating their possible role in wound healing. Corneal stromal cell differentiation is associated with the activation of diverse molecular pathways critical for the repair of fibroblasts and myofibroblasts. Identified proteins such as profilin 1 and talin could play a tentative role in corneal healing and serve as a potential target to treat corneal fibrosis.

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Section: Discussionmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Quantitative Proteomics Reveals Molecular Network Driving Stromal Cell Differentiation: Implications for Corneal Wound Healing

Nishtala

Panigrahi

Shetty

et al. 2022

IJMS

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“…With the increasing availability of noninvasive commercially available instruments to measure corneal biomechanical properties, a number of investigators have reported the changes induced on corneal biomechanical behavior after refractive surgery. 3,12,13,18,24…”

Section: Discussionmentioning

confidence: 99%

Changes in Corneal Biomechanical Properties After Small-Incision Lenticule Extraction and Photorefractive Keratectomy, Using a Noncontact Tonometer

Zarei‐Ghanavati

Jafarpour

Hassanzadeh

et al. 2021

Cornea

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Purpose:The aim of this study was to evaluate and compare early corneal biomechanical changes after small-incision lenticule extraction (SMILE) and photorefractive keratectomy (PRK).Methods:The study comprised 74 patients eligible for refractive surgery, equally allocated to PRK (37 patients) and SMILE (37 patients). Corneal biomechanical properties were recorded and compared between the 2 groups at preoperatively and 3 months after surgery using a dynamic ultra-high-speed Scheimpflug camera equipped with a noncontact tonometer.Results:Both procedures significantly affected corneal biomechanical properties at 3 months after surgery. Patients in the PRK group showed significantly better results for deformation amplitude ratio (DA ratio) (P = 0.03), maximum inverse radius (InvRadMax) (P = 0.02), and A2 time (P = 0.03). The mean changes in DA ratio, HC radius, InvRadMax, and Ambrosio relational thickness were significantly higher in the SMILE group in comparison with those of the PRK group (all, P < 0.05). In both groups, change in CCT was significantly correlated with changes in DA ratio and InvRadMax (P < 0.05).Conclusions:sBoth SMILE and PRK refractive surgeries significantly altered corneal biomechanical properties but the changes were more prominent after SMILE.

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“…8,98 Lesser inflammatory response in wound healing after SMILE may contribute to better biomechanics. 64,99 The difference tended to be more significant in patients with high myopia, 94,100 probably because thinner flap and larger ablation depth affected more anterior stroma in LASIK. 8 In conclusion, this study has reviewed as many articles as possible and the differences between the three refractive surgeries are summarized in the Table . The applicability, efficacy, safety, and predictability between the three different techniques were comparable in most populations.…”

Section: Lasik Vs Smilementioning

confidence: 99%

Comparison of clinical outcomes of LASIK, Trans-PRK, and SMILE for correction of myopia

Chang

Lin

Hsu

et al. 2022

Journal of the Chinese Medical Association

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Transepithelial photorefractive keratectomy (Trans-PRK), laser-assisted in situ keratomileusis (LASIK), and small incision lenticule extraction (SMILE) are three mainstay refractive surgeries worldwide. The applicability, efficacy, safety, and predictability of these different techniques are quite similar. Trans-PRK has the strongest biostability, earliest return to normal corneal sensitivity but the longest recovery time, most uncomfortable postoperative experience, and possibility of corneal haze. LASIK possesses the fastest visual rehabilitation but the slowest corneal nerve reinnervation, and flap displacement is possibly lifelong. SMILE incurs no flap-related complications and has intermediate vision recovery time and biomechanics compared with Trans-PRK and LASIK. However, it lacks the cyclotorsion-compensation system, eye-tracking system, and customized treatment profile for high astigmatism or irregular corneal surface. This review aims to introduce the mechanisms, pros, and cons of these three types of refractive surgery. With full understanding, practitioners could advise patients on the most suitable treatment of choice.

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Early biological responses in ocular tissue after SMILE and LASIK surgery

Cited by 10 publications

References 47 publications

Quantitative Proteomics Reveals Molecular Network Driving Stromal Cell Differentiation: Implications for Corneal Wound Healing

Quantitative Proteomics Reveals Molecular Network Driving Stromal Cell Differentiation: Implications for Corneal Wound Healing

Changes in Corneal Biomechanical Properties After Small-Incision Lenticule Extraction and Photorefractive Keratectomy, Using a Noncontact Tonometer

Comparison of clinical outcomes of LASIK, Trans-PRK, and SMILE for correction of myopia

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