E‐cadherin‐mediated contact of endothelial progenitor cells with mesenchymal stem cells through β‐catenin signaling

Xia, Jie; Zhang, Hongwei; Gao, Xiaopeng; Guo, Jun; Hou, Jixue; Wang, Xiaoyi; Wang, Sibo; Yang, Tao; Zhang, Xuyong; Ge, Quanhu; Wan, Longfei; Cheng, Wenzhe; Zheng, Jinpo; Chen, Xueling; Wu, Xiangwei

doi:10.1002/cbin.10579

Cited by 7 publications

(7 citation statements)

References 26 publications

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“…We have previously reported that MSCs and EPCs adhere to each other in the bone marrow cavity and in vitro ( 13 , 14 ), and that this mutual adhesion is important for the biological functions of both cell types. EPCs have also been reported to promote the differentiation of MSCs into osteoblasts ( 15 ).…”

Section: Resultsmentioning

confidence: 99%

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VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms

et al. 2017

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Stem cell therapy is a promising treatment strategy for ischemic diseases. Mesenchymal stem cells (MSCs) and endothelial progenitor cells (EPCs) adhere to each other in the bone marrow cavity and in in vitro cultures. We have previously demonstrated that the adhesion between MSCs and EPCs is critical for MSC self-renewal and their multi-differentiation into osteoblasts and chondrocytes. In the present study, the influence of the indirect communication between EPCs and MSCs on the endothelial differentiation potential of EPCs was investigated, and the molecular mechanisms underlying MSC-mediated EPC differentiation were explored. The effects of vascular endothelial growth factor (VEGF), which is secreted by MSCs, on EPC differentiation via paracrine mechanisms were examined via co-culturing MSCs and EPCs. Reverse transcription-quantitative polymerase chain reaction and western blot analysis were used to detect the expression of genes and proteins of interest. The present results demonstrated that co-culturing EPCs with MSCs enhanced the expression of cluster of differentiation 31 and von Willebrand factor, which are specific markers of an endothelial phenotype, thus indicating that MSCs may influence the endothelial differentiation of EPCs in vitro. VEGF appeared to be critical to this process. These findings are important for the understanding of the biological interactions between MSCs and EPCs, and for the development of applications of stem cell-based therapy in the treatment of ischemic diseases.

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Section: Resultsmentioning

confidence: 99%

“…BMMSCs were isolated using the technique reported in our previous studies ( 13 , 14 ). Briefly, bone marrow cells were collected from 6-week-old wild-type male C57BL/6 mice euthanized by cervical dislocation.…”

Section: Methodsmentioning

confidence: 99%

VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms

et al. 2017

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Section: Discussionmentioning

confidence: 99%

“…[94] The symbiotic relationship of the coculture has also been illustrated by the mutual attachment of MSCs and ECs (particularly EPCs), which augments the pluripotency of MSCs and simultaneously promotes angiogenesis. [95,161] Under optimal conditions, VEGF production by MSCs can be increased. The use of strontium-containing titanium material was reported to stimulate MSCs toward higher secretion of VEGF and platelet-derived growth factor-BB, which are both essential for angiogenesis.…”

Section: Angiogenic Response Of Ecs Mediated Through Other Cell Typesmentioning

confidence: 99%

Inorganic Agents for Enhanced Angiogenesis of Orthopedic Biomaterials

Šalandová

Hengel

Apachitei

et al. 2021

Adv Healthcare Materials

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Aseptic loosening of a permanent prosthesis remains one of the most common reasons for bone implant failure. To improve the fixation between implant and bone tissue as well as enhance blood vessel formation, bioactive agents are incorporated into the surface of the biomaterial. This study reviews and compares five bioactive elements (copper, magnesium, silicon, strontium, and zinc) with respect to their effect on the angiogenic behavior of endothelial cells (ECs) when incorporated on the surface of biomaterials. Moreover, it provides an overview of the state-of-the-art methodologies used for the in vitro assessment of the angiogenic properties of these elements. Two databases are searched using keywords containing ECs and copper, magnesium, silicon, strontium, and zinc. After applying the defined inclusion and exclusion criteria, 59 articles are retained for the final assessment. An overview of the angiogenic properties of five bioactive elements and the methods used for assessment of their in vitro angiogenic potential is presented. The findings show that silicon and strontium can effectively enhance osseointegration through the simultaneous promotion of both angiogenesis and osteogenesis. Therefore, their integration onto the surface of biomaterials can ultimately decrease the incidence of implant failure due to aseptic loosening.

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“…This diversity depends on glycan composition and complexity of branching, which is cell type-specific [340,341]. Glycosylation regulates the biological activity of proteins, such as cell-cell and cell-extracellular matrix interactions, ligand binding affinity, protein trafficking as well as subcellular localization and protein stability [177,[342][343][344]. The IR, for example, is highly glycosylated in the ectodomains of the α and β subunit [345][346][347][348][349][350][351], which is essential for proper processing of the receptor [347,349,350].…”

Section: Protein Glycosylationmentioning

confidence: 99%

Identification and characterization of adipose surface epitopes

Onogi

Khalil

Ussar

2020

Biochemical Journal

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Adipose tissue is a central regulator of metabolism and an important pharmacological target to treat the metabolic consequences of obesity, such as insulin resistance and dyslipidemia. Among the various cellular compartments, the adipocyte cell surface is especially appealing as a drug target as it contains various proteins that when activated or inhibited promote adipocyte health, change its endocrine function and eventually maintain or restore whole-body insulin sensitivity. In addition, cell surface proteins are readily accessible by various drug classes. However, targeting individual cell surface proteins in adipocytes has been difficult due to important functions of these proteins outside adipose tissue, raising various safety concerns. Thus, one of the biggest challenges is the lack of adipose selective surface proteins and/or targeting reagents. Here, we discuss several receptor families with an important function in adipogenesis and mature adipocytes to highlight the complexity at the cell surface and illustrate the problems with identifying adipose selective proteins. We then discuss that, while no unique adipocyte surface protein might exist, how splicing, posttranslational modifications as well as protein/protein interactions can create enormous diversity at the cell surface that vastly expands the space of potentially unique epitopes and how these selective epitopes can be identified and targeted.

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E‐cadherin‐mediated contact of endothelial progenitor cells with mesenchymal stem cells through β‐catenin signaling

Cited by 7 publications

References 26 publications

VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms

VEGF secreted by mesenchymal stem cells mediates the differentiation of endothelial progenitor cells into endothelial cells via paracrine mechanisms

Inorganic Agents for Enhanced Angiogenesis of Orthopedic Biomaterials

Identification and characterization of adipose surface epitopes

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