Dynamics and 3D organization of secretory organelles of Toxoplasma gondii

Paredes-Santos, Tatiana C.; Souza, Wanderley de; Attias, Márcia

doi:10.1016/j.jsb.2011.11.028

Cited by 68 publications

(105 citation statements)

References 37 publications

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“…Fig. 3B also shows a 200 nm blob on the parasite surface that is compatible with the diameter of dense granules observed in ultrathin sections by TEM (Dubremetz et al, 1993;Paredes-Santos et al, 2012). However, at this point this assumption is very speculative.…”

Section: The Content Of the Parasitophorous Vacuolesupporting

confidence: 74%

New views of the Toxoplasma gondii parasitophorous vacuole as revealed by Helium Ion Microscopy (HIM)

Souza

Attias

2015

Journal of Structural Biology

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Section: The Content Of the Parasitophorous Vacuolesupporting

confidence: 74%

New views of the Toxoplasma gondii parasitophorous vacuole as revealed by Helium Ion Microscopy (HIM)

Souza

Attias

2015

Journal of Structural Biology

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“…The micronemes are first to discharge upon binding to the host cell. The club-shaped rhoptries, which occupy a large cellular area with the thinner neck portions (Figure 1) oriented toward the apical end of the cell [43], then discharge and mediate entry of the parasite to the host cell. The evolutionary origins of these organelles has been murky, but the most strongly supported hypothesis [36,44–46] is of a highly divergent endolysosomal origin.…”

Section: Rhoptries and Micronemes: Divergent Endolysosomal Homologues?mentioning

confidence: 99%

Cryptic organelle homology in apicomplexan parasites: insights from evolutionary cell biology

Klinger

Nisbet

Ouologuem

et al. 2013

Current Opinion in Microbiology

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The economic and clinical significance of apicomplexan parasites drives interest in their many evolutionary novelties. Distinctive intracellular organelles play key roles in parasite motility, invasion, metabolism, and replication, and understanding their relationship with the organelles of better-studied eukaryotic systems suggests potential targets for therapeutic intervention. Recent work has demonstrated divergent aspects of canonical eukaryotic components in the apicomplexa, including Golgi bodies and mitochondria. The apicoplast is a relict plastid of secondary endosymbiotic origin, harboring metabolic pathways distinct from those of host species. The inner membrane complex is derived from the cortical alveoli defining the superphylum Alveolata, but in apicomplexans functions in parasite motility and replication. Micronemes and rhoptries are associated with establishment of the intracellular niche, and define the apical complex for which the phylum is named. Morphological, cell biological and molecular evidence strongly suggest that these organelles are derived from the endocytic pathway.

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“…15 Previously, we reported that T. gondii infection suppressed apoptosis of HeLa cells for 36hours post-infection in uninfected cells and in cells treated with actinomycin D. 16 T. gondii uses a mixture of specialized parasite secreted proteins, including ROPs, GRAs and MICs to invade cells. 17,18 Many of these parasite secreted proteins are anchored on the parasitophorous vacuole membrane (PVM) and can interact with the host cells directly. The kinase activity ROPs, such as ROP2, 19 …”

Section: Discussionmentioning

confidence: 99%

Increased Expression of Toxoplasma Gondii GRA1 Suppresses Host Cell Apoptosis

Chen¹

2017

JBMOA

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Suppression of host cell apoptosis enable Toxoplasma gondii to proliferate, but the mechanism is not well understood. We explore the relationship between the expression of T. gondii dense granule protein 1 (GRA1) and host HeLa cell apoptosis. We inserted the T. gondii gra1 coding gene into a pET32a vector and produced recombinant GRA1 (rGRA1) in E. coli Rosetta strain. We then immunized rabbits with rGRA1 to produce anti-GRA1 serum and infected HeLa cells with T. gondii tachyzoites. We determined the expression of T. gondii GRA1 by Western blotting with anti-GRA1 serum and determined apoptosis of HeLa cells via the Annexin V-FITC/PI method. All the experiments were repeated for three times in the same condition. The expression of GRA1 decreased gradually after T. gondii infection. The rate of HeLa cell apoptosis increased more rapidly in the infected cells than in the uninfected cells. Our results suggest that the suppression of host cell apoptosis is related to the expression of T. gondii GRA1 expression.

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Dynamics and 3D organization of secretory organelles of Toxoplasma gondii

Cited by 68 publications

References 37 publications

New views of the Toxoplasma gondii parasitophorous vacuole as revealed by Helium Ion Microscopy (HIM)

New views of the Toxoplasma gondii parasitophorous vacuole as revealed by Helium Ion Microscopy (HIM)

Cryptic organelle homology in apicomplexan parasites: insights from evolutionary cell biology

Increased Expression of Toxoplasma Gondii GRA1 Suppresses Host Cell Apoptosis

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