Dynamic visualization of type II peptidyl carrier protein recognition in pyoluteorin biosynthesis

Abstract

The interface interactions of a type II peptidyl carrier protein and partner enzyme are observed to be unique and dynamic.

Structure and Mechanistic Analyses of the Gating Mechanism of Elongating Ketosynthases

“…coli’s rapid homeoviscous adaptive response to regulate membrane fluidity in response to reductions in temperature. ,,− Therefore, we developed a synthetic route for a C16:1-α-bromo-pantetheinamide unsaturated crosslinking probe (Figure ), designed to mimic FabF’s privileged substrate, palmitoleoyl-ACP (Figure S3). As with other crosslinkers developed to trap ACP–PE interactions, ,− ,− we replaced the labile thioester in the native substrate with an amide linkage, which is necessary to trap crosslinked ACP–PE complexes. However, recent NMR data from our group suggests that the identity of the linkage affects the overall structure of AcpP in solution. These differences are mostly due to altered sequestration − of non-natively tethered substrates and likely minimally affect the conformation and structure in crosslinked complexes.…”

Total synthesis and mechanism of action of the antibiotic armeniaspirol A

“…Armeniaspirols are part of a larger family of natural products that all carry chlorinated pyrrole moieties. The compounds have been isolated from diverse origins, as exemplified by pyoluteorin, a widely studied signaling molecule from Gram-negative Pseudomonas sp., 33 pyralomicin from Nonomuraea spiralis , 34 pyrrolomycin and streptopyrrole from terrestrial Streptomyces sp., 35,36 or chlorizidine 37 and marinopyrrole A 38 from marine Streptomyces sp. Their individual scaffolds and the underlying biosynthesis are different ( Fig.…”

“…The compounds have been isolated from diverse origins, as exemplified by pyoluteorin, a widely studied signaling molecule from Gram-negative Pseudomonas sp, 33 pyralomicin from Nonmuraea spiralis, 34 pyrrolomycin and streptopyrrole from terrestrial Streptomyces sp, 35,36 or chlorizidine 37 and marinopyrrole A 38 from marine Streptomyces sp. Their individual scaffolds and the underlying biosynthesis are different (Figure 4), however, they do not only share a chloro-pyrrole as a common feature, but also an acylated phenol moiety.…”

In silico identification and in vitro evaluation of a protein‐protein interaction inhibitor of Escherichia coli fatty acid biosynthesis

“…Aiding in the discovery and design of inhibitors, the past decade has seen a large advance in the structural knowledge of the FAS‐II system. Our group and others have succeeded in characterizing many E. coli ACP (EcACP) protein‐protein interactions using X‐ray crystallography, protein NMR and other biophysical techniques (Corpuz et al., 2020; Dodge et al., 2019; Du et al., 2020; Jain et al., 2004; Mindrebo et al., 2020; Misson et al., 2020; Nguyen et al., 2014; Tallorin et al., 2016; Zhang et al., 2003). In addition to the wealth of structural knowledge of EcACP·partner enzyme interactions, we also recently published a fluorescent‐based in vitro assay to rapidly monitor PPIs between EcACP and thirteen different partner enzymes (Charov & Burkart, 2019, 2020).…”