Dynamic responses of prolactin, growth hormone and their receptors to hyposmotic acclimation in the olive flounder Paralichthys olivaceus

Yuan, Mingzhe; Jia, Qiong; Wang, Ting; Lu, Qi; Tang, Langlang; Wang, Youji; Lu, Wei

doi:10.1016/j.ygcen.2017.09.005

Cited by 15 publications

(17 citation statements)

References 36 publications

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“…Yoko et al research showed that PRLR2 facilitates acclimation responses to increased extracellular osmolality [46]. Yuan et al observed the synchronous expression trend of the renal PRLR with pituitary PRL (5d) and the asynchronous expression peaks between branchial (8d) and renal PRLR (5d) in olive flounder Paralichthys olivaceus, and the mRNA levels of PRL receptor (PRLR) in both gill and kidney displayed a similar trend to the pituitary PRL [47]. Andre et al reported that TRPV4 expression increased by 48 h after transfer from freshwater to seawater and declined as early as 6 h after transfer from seawater to freshwater, indicating TRPV4 expression maybe regulated through the same second messenger pathways involved in hyposmotically-induced PRL release [48].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic response to three osmotic stresses in gills of hybrid tilapia (Oreochromis mossambicus female × O. urolepis hornorum male)

Zhu

et al. 2020

BMC Genomics

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Background: Osmotic stress is a widespread phenomenon in aquatic animal. The ability to cope with salinity stress and alkaline stress is quite important for the survival of aquatic species under natural conditions. Tilapia is an important commercial euryhaline fish species. What's more tilapia is a good experimental material for osmotic stress regulation research, but the molecular regulation mechanism underlying different osmotic pressure of tilapia is still unexplored. Results: To elucidate the osmoregulation strategy behind its hyper salinity, alkalinity and salinity-alkalinity stress of tilapia, the transcriptomes of gills in hybrid tilapia (Oreochromis mossambicus ♀ × O. urolepis hornorum ♂) under salinity stress (S: 25‰), alkalinity stress(A: 4‰) and salinity-alkalinity stress (SA: S: 15‰, A: 4‰) were sequenced using deep-sequencing platform Illumina/HiSeq-2000 and differential expression genes (DEGs) were identified. A total of 1958, 1472 and 1315 upregulated and 1824, 1940 and 1735 downregulated genes (P-value < 0.05) were identified in the salt stress, alkali stress and saline-alkali stress groups, respectively, compared with those in the control group. Furthermore, Kyoto Encyclopedia of Genes and Genomes pathway analyses were conducted in the significant different expression genes. In all significant DEGs, some of the typical genes involved in osmoregulation, including carbonic anhydrase (CA), calcium/calmodulin-dependent protein kinase (CaM kinase) II (CAMK2), aquaporin-1(AQP1), sodium bicarbonate cotransporter (SLC4A4/NBC1), chloride channel 2(CLCN2), sodium/ potassium/chloride transporter (SLC12A2 / NKCC1) and other osmoregulation genes were also identified. RNA-seq results were validated with quantitative real-time PCR (qPCR), the 17 random selected genes showed a consistent direction in both RNA-Seq and qPCR analysis, demonstrated that the results of RNA-seq were reliable. Conclusions:The present results would be helpful to elucidate the osmoregulation mechanism of aquatic animals adapting to saline-alkali challenge. This study provides a global overview of gene expression patterns and pathways that related to osmoregulation in hybrid tilapia, and could contribute to a better understanding of the molecular regulation mechanism in different osmotic stresses.

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Section: Discussionmentioning

confidence: 99%

Transcriptomic response to three osmotic stresses in gills of hybrid tilapia (Oreochromis mossambicus female × O. urolepis hornorum male)

Zhu

et al. 2020

BMC Genomics

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“…To reduce the potential influence of sexual dimorphism in fish (Yuan et al, 2017), gynogenetic olive flounder (body weights: 500 ± 50 g) were produced and reared in SW (30‰) recirculating aquaculture systems at the Central Experimental Station of Chinese Academy of Fisheries Sciences in November 2018 (Beidaihe, Hebei, China). About 80 active coping (AC) individuals and 80 passive coping (PC) individuals were selected from PC and AC broodstock screened according to behavioral tests developed by Rupia et al (2016).…”

Section: Methodsmentioning

confidence: 99%

“…The mean light intensity, measured centrally at the bottom of each tank, was approximately 40 lux. Fish were starved throughout the experiment to ensure that differential rates of feeding did not influence the results of the study (Yuan et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

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Differential Coping Strategies in Response to Salinity Challenge in Olive Flounder

Zeng

Herbert

2019

Front. Physiol.

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To examine how different fish coping strategies respond to salinity challenge, olive flounder (Paralichthys olivaceus) with active coping style (AC) and passive coping style (PC) were transferred from seawater (SW) to freshwater (FW) and their behavior and physiology were analyzed. Different behavioral coping strategies, in terms of escape and feeding tendencies, were confirmed in AC and PC fish without FW exposure. Differences in swimming distance between AC and PC flounder were then assessed after 1 and 2 days of FW transfer. Plasma parameters and branchial gene expression were also determined 2, 5, 8, and 14 days after transfer, with comparisons between AC and PC fish and against a SW-acclimated control group. The results showed that: (1) PC flounder exhibited a significant reduction in swimming activity, while AC flounder significantly increased locomotion 2 days after transfer. (2) The plasma osmolality and plasma ionic (Na+ and Cl−) concentration of FW-acclimated PC flounder declined in a continuous fashion over time but this contrasted against the plasma parameters of AC flounder which fluctuated below the baseline level of a SW-acclimated control group. (3) The expression of NKA-α1 and NHE-3-like mRNA in PC flounder gill increased significantly from 5 days, but the expression of these two genes in AC flounder only increased after 8 days of transfer. (4) There were no remarkable differences observed in Rhcg expressions between AC and PC flounder. This study indicates for the first time that PC flounder adopt a “freeze-passive tolerance” strategy while AC flounder adopt a “flight-active resistance” defense strategy in response to salinity challenge.

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“…The CNSS mRNA expression levels were analyzed by quantitative real-time PCR on ABI 7500 Real-Time PCR System (Applied Biosystems, Singapore). Relative quantification of the target gene transcripts was analyzed using β-actin gene expression as the reference gene (Yuan et al, 2017). Sequences of CRH, UI, UII were obtained from GeneBank.…”

Section: Relative Quantitative Rt-pcrmentioning

confidence: 99%

Dynamic Responses of the Caudal Neurosecretory System (CNSS) Under Thermal Stress in Olive Flounder (Paralichthys olivaceus)

Yuan

Long

et al. 2020

Front. Physiol.

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Temperature is a critical environmental factor that affect most biological and physiological processes in fish. The caudal neurosecretory system (CNSS) is unique to fish and is proved to maintain homeostasis during seasonal alterations. However, the dynamic expression and secretion pattern of its major hormones, corticotrophinreleasing hormone (CRH), urotensin I (UI), and urotensin II (UII), and their response to thermal stress has not been studied. CRH, UII and cortisol in plasma, gene expression levels of CRH, UI, and UII in the CNSS of olive flounder (Paralichthys olivaceus) were therefore characterized. UI-and UII-positive Dahlgren cells, as well as cell proliferation in the CNSS, were also quantified. The results showed that plasma cortisol and CRH were increased in both low temperature (LT) and high temperature (HT) groups. However, there was no difference in plasma UI and UII during thermal stress. In CNSS, CRH, UI, and UII mRNA levels were all significantly elevated in response to acute hypothermal stress and recovered back to the control (normal) level after 8 days of adaptation. During hyperthermal challenge, gene expression of CRH and UI only significantly increased after 8-days of transfer but no change in UII was observed. We also demonstrated an increasing percent of UI-positive Dahlgren cells in the CNSS of 8-days hyperthermal stressed fish. However, no BrdU-labeled Dahlgren cells were found among the three treatment groups. Collectively, our results demonstrate that the CNSS is subjected to dynamic responses under thermal stress and expands upon the role of the CNSS in thermoregulation. The dynamic responses of hormone levels and the gene expression of CRH, UI and UII in CNSS are all involved in the process of hyper-or hypo-thermal stress and adaptation.

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Dynamic responses of prolactin, growth hormone and their receptors to hyposmotic acclimation in the olive flounder Paralichthys olivaceus

Cited by 15 publications

References 36 publications

Transcriptomic response to three osmotic stresses in gills of hybrid tilapia (Oreochromis mossambicus female × O. urolepis hornorum male)

Transcriptomic response to three osmotic stresses in gills of hybrid tilapia (Oreochromis mossambicus female × O. urolepis hornorum male)

Differential Coping Strategies in Response to Salinity Challenge in Olive Flounder

Dynamic Responses of the Caudal Neurosecretory System (CNSS) Under Thermal Stress in Olive Flounder (Paralichthys olivaceus)

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