Dynamic DNA nanotechnology: toward functional nanoscale devices

DeLuca, Marcello; Shi, Ze; Castro, Carlos E.; Arya, Gaurav

doi:10.1039/c9nh00529c

Cited by 177 publications

(188 citation statements)

References 172 publications

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“…However, to date the great number of all presented DNA constructions have been static (usually on purpose), and therefore, the coming of age of the mechanically moving more complex DNA nanostructures has only recently been witnessed. 160 That also means there are a great deal of challenges ahead in creating dynamic DNA devices, such as their stability under application-specific conditions.…”

Section: Discussionmentioning

confidence: 99%

Robotic DNA Nanostructures

et al. 2020

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Over the past decade, DNA nanotechnology has spawned a broad variety of functional nanostructures tailored toward the enabled state at which applications are coming increasingly in view. One of the branches of these applications is in synthetic biology, where the intrinsic programmability of the DNA nanostructures may pave the way for smart task-specific molecular robotics. In brief, the synthesis of the user-defined artificial DNA nano-objects is based on employing DNA molecules with custom lengths and sequences as building materials that predictably assemble together by obeying Watson–Crick base pairing rules. The general workflow of creating DNA nanoshapes is getting more and more straightforward, and some objects can be designed automatically from the top down. The versatile DNA nano-objects can serve as synthetic tools at the interface with biology, for example, in therapeutics and diagnostics as dynamic logic-gated nanopills, light-, pH-, and thermally driven devices. Such diverse apparatuses can also serve as optical polarizers, sensors and capsules, autonomous cargo-sorting robots, rotary machines, precision measurement tools, as well as electric and magnetic-field directed robotic arms. In this review, we summarize the recent progress in robotic DNA nanostructures, mechanics, and their various implementations.

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Section: Discussionmentioning

confidence: 99%

Robotic DNA Nanostructures

et al. 2020

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“… 31 Actuation of such devices has been achieved through toehold-mediated strand displacement, 38 as well as thermal, electric, magnetic, salt-based, pH-based, light-based, and aptamer-based actuation. 39 We direct the reader to the excellent review by DeLuca et al on the design and methodologies of dynamic DNA structures 39 and the review by Castro et al on how these systems can be leveraged for molecular-scale precision measurements. 40 …”

Section: Dna Nanotechnologymentioning

confidence: 99%

Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics

et al. 2020

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DNA nanotechnology has proven exceptionally apt at probing and manipulating biological environments as it can create nanostructures of almost arbitrary shape that permit countless types of modifications, all while being inherently biocompatible. Emergent areas of particular interest are applications involving cellular membranes, but to fully explore the range of possibilities requires interdisciplinary knowledge of DNA nanotechnology, cell and membrane biology, and biophysics. In this review, we aim for a concise introduction to the intersection of these three fields. After briefly revisiting DNA nanotechnology, as well as the biological and mechanical properties of lipid bilayers and cellular membranes, we summarize strategies to mediate interactions between membranes and DNA nanostructures, with a focus on programmed delivery onto, into, and through lipid membranes. We also highlight emerging applications, including membrane sculpting, multicell self-assembly, spatial arrangement and organization of ligands and proteins, biomechanical sensing, synthetic DNA nanopores, biological imaging, and biomelecular sensing. Many critical but exciting challenges lie ahead, and we outline what strikes us as promising directions when translating DNA nanostructures for future in vitro and in vivo membrane applications.

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“…[ 4,5 ] Dynamic DNA reactions (e.g., strand displacement) permit the implantation of stimuli‐responsive units on the drug delivery carriers. [ 6,7 ] Nevertheless, the poor stability of DNA molecule under physiological conditions caused by low divalent cation concentration and the nucleases degradation highlights the importance of enhancing their structural stability. The biomedical applications of these frameworks are also limited by the slow internalization kinetics that is mainly caused by the negative charge of nucleic acids.…”

Section: Figurementioning

confidence: 99%

Rational Design of DNA Framework‐Based Hybrid Nanomaterials for Anticancer Drug Delivery

Wang

Irfan

et al. 2020

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Engineered DNA frameworks have been extensively exploited as affinity scaffolds for drug delivery. However, few studies focus on the rational design to comprehensively improve their stability, internalization kinetics, and drug loading efficiency. Herein, DNA framework‐based hybrid nanomaterials are rationally engineered by using a molecular docking tool, where the framework acts as a template to support conjugated polymers. The hybrid materials exhibit high stability in biofluids owning to the multiple interactions between DNA and cationic conjugated polymer. Through molecular docking, it is found that a specific structure of the conjugated polymer at major grooves of DNA gives rise to a unique pocket for small‐molecular drug doxorubicin (DOX) yielding lower binding energy than conventional DOX binding sites. This increases the binding affinity of DOX, allowing for high drug loading content and efficiency, and preventing drug leakage under physiological condition. As a proof of concept, the hybrid nanomaterials equipped with aptamer are used to carry DOX and antisense oligonucleotide G3139, which effectively inhibits solid tumor growth and shows negligible side effects on mice. It is anticipated that this approach would find broad applications in hybrid materials design and precise medicine.

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Dynamic DNA nanotechnology: toward functional nanoscale devices

Abstract: This review presents recent advances and continuing challenges in the design, characterization, and modelling of dynamic DNA nanodevices.

Cited by 177 publications

References 172 publications

Robotic DNA Nanostructures

Robotic DNA Nanostructures

Emerging applications at the interface of DNA nanotechnology and cellular membranes: Perspectives from biology, engineering, and physics

Rational Design of DNA Framework‐Based Hybrid Nanomaterials for Anticancer Drug Delivery

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