Dynamic compression of cartilage constructs engineered from expanded human articular chondrocytes

Démarteau, Olivier; Wendt, David; Braccini, Alessandra; Jakob, Marcel; Schäfer, Dirk; Heberer, Michael; Martin, Ivan

doi:10.1016/j.bbrc.2003.09.099

Cited by 169 publications

(124 citation statements)

References 38 publications

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“…On the one hand, more developed engineered cartilage could support enhanced repair, due to increased retention of donor cells in the repair tissue, 5 and more physiological transduction of loading to the cells. 37 On the other hand, less developed engineered cartilage could integrate more efficiently with native cartilage, due to higher accessibility of cells at the interface. 38 Taken together, these findings indicate the need to identify an appropriate degree of tissue development that guarantees at the same time cell protection, appropriate response to loading, as well as integrative capacity.…”

Section: Discussionmentioning

confidence: 99%

Cartilage tissue engineering by expanded goat articular chondrocytes

Miot

Freitas

Wirz

et al. 2006

Journal Orthopaedic Research

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In this study we investigated whether expanded goat chondrocytes have the capacity to generate cartilaginous tissues with biochemical and biomechanical properties improving with time in culture. Goat chondrocytes were expanded in monolayer with or without combinations of FGF-2, TGF-b1, and PDGFbb, and the postexpansion chondrogenic capacity assessed in pellet cultures. Expanded chondrocytes were also cultured for up to 6 weeks in HYAFF 1 -M nonwoven meshes or Polyactive TM foams, and the resulting cartilaginous tissues were assessed histologically, biochemically, and biomechanically. Supplementation of the expansion medium with FGF-2 increased the proliferation rate of goat chondrocytes and enhanced their postexpansion chondrogenic capacity. FGF-2-expanded chondrocytes seeded in HYAFF 1 -M or Polyactive TM scaffolds formed cartilaginous tissues with wet weight, glycosaminoglycan, and collagen content, increasing from 2 days to 6 weeks culture (up to respectively 2-, 8-, and 41-fold). Equilibrium and dynamic stiffness measured in HYAFF 1 -M-based constructs also increased with time, up to, respectively, 1.3-and 16-fold. This study demonstrates the feasibility to engineer goat cartilaginous tissues at different stages of development by varying culture time, and thus opens the possibility to test the effect of maturation stage of engineered cartilage on the outcome of cartilage repair in orthotopic goat models. ß

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Section: Discussionmentioning

confidence: 99%

Cartilage tissue engineering by expanded goat articular chondrocytes

Miot

Freitas

Wirz

et al. 2006

Journal Orthopaedic Research

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“…The field is further complicated by the fact that the effects of mechanical stimuli on tissue engineered cartilage may vary substantially using different scaffold systems [208] or constructs at different stages of development [209]. Moreover, a crucial issue that still remains to be demonstrated is whether in vitro mechanical preconditioning of engineered cartilage would result in grafts with increased chances of long-term in vivo success [210].…”

Section: Bioreactors To Establish and Maintain 3d Culturesmentioning

confidence: 99%

Cartilage tissue engineering for degenerative joint disease☆

Nešić

Whiteside

Brittberg

et al. 2006

Advanced Drug Delivery Reviews

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Pain in the joint is often due to cartilage degeneration and represents a serious medical problem affecting people of all ages. Although many, mostly surgical techniques, are currently employed to treat cartilage lesions, none has given satisfactory results in the long term. Recent advances in biology and material science have brought tissue engineering to the forefront of new cartilage repair techniques. The combination of autologous cells, specifically designed scaffolds, bioreactors, mechanical stimulations and growth factors together with the knowledge that underlies the principles of cell biology offers promising avenues for cartilage tissue regeneration. The present review explores basic biology mechanisms for cartilage reconstruction and summarizes the advances in the tissue engineering approaches. Furthermore, the limits of the new methods and their potential application in the osteoarthritic conditions are discussed.

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“…Im Umkehrschluss führt eine verminderte Mobilisierung von Gelenken zu einer Atrophie des Knorpels, die sich in einem Verlust der Proteoglykane äußert (Palmoski et al, 1979). Diese interindividuelle Variabilität im Umgang mit humanen Chondrozyten unter mechanostimulatorischen Einflüssen ist vorbeschrieben (Barbero et al, 2002;Demarteau et al, 2003) und ein Hauptproblem, das es zu lösen gilt auf dem Weg zu reproduzierbaren und standardisierten Tissue Engineering-Verfahren.…”

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