Dynamic aspects of ovarian superoxide dismutase isozymes during the ovulatory process in the rat

Sato, Eisuke F.; Kobuchi, Hirotsugu; Edashige, Keisuke; Takahashi, Masahiko; Yoshioka, Tamotsu; Utsumi, Kozo; Inoue, Masayasu

doi:10.1016/0014-5793(92)80502-8

Cited by 68 publications

(9 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Functional ovarian damage in obese females has been associated with abnormal levels of antioxidant enzymes (Lim and Luderer, 2011), and the mammalian ovary possesses antioxidant defenses, including ROS-scavenging enzymes such as CAT, GSH-Px, and SOD (Sato et al, 1992;Hsieh et al, 1997). CAT catalyzes the decomposition of H 2 O 2 into H 2 O and O 2 to protect cells from H 2 O 2 toxicity (Sun et al, 2013).…”

Section: Discussionmentioning

confidence: 99%

Phycocyanin Improves Reproductive Ability in Obese Female Mice by Restoring Ovary and Oocyte Quality

Wen

Han

Liu

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

Section: Discussionmentioning

confidence: 99%

Phycocyanin Improves Reproductive Ability in Obese Female Mice by Restoring Ovary and Oocyte Quality

Wen

Han

Liu

et al. 2020

Front. Cell Dev. Biol.

View full text Add to dashboard Cite

“…Mammalian ovaries possess antioxidant defenses including the antioxidant tripeptide glutathione (GSH), and ROS-scavenging enzymes such as SOD, GSH-Px, CAT, glutathione S-transferase (GST), and peroxiredoxin (PRDX) [ 4 , 56 , 57 ]. We hypothesized that ovarian aging is associated with decreased expression of ovarian antioxidant genes and lower antioxidant enzyme levels, resulting in oxidative damage to ovarian function.…”

Section: Discussionmentioning

confidence: 99%

C-phycocyanin protects against low fertility by inhibiting reactive oxygen species in aging mice

Han

et al. 2016

Oncotarget

View full text Add to dashboard Cite

Women over 35 have higher rates of infertility, largely due to deterioration of oocyte quality characterized by fragmentation, abnormal meiotic spindle-chromosome complexes, and oxidative stress. C-phycocyanin (PC) is a biliprotein enriched in Spirulina platensis that is known to possess antioxidant, anti-inflammatory, and radical-scavenging properties. D-galactose-induced aging acceleration in mice has been extensively used to study aging mechanisms and for pharmaceutical screening. In this study, adult female B6D2F/1 mice injected with D-galactose were used as a model to test the age-reversing effects of PC on degenerated reproductive ability. Our results show that PC can prevent oocyte fragmentation and aneuploidy by maintaining cytoskeletal integrity. Moreover, PC can reverse the expression of antioxidant genes, increase superoxide dismutase (SOD) activity and decrease methane dicarboxylic aldehyde (MDA) content, and normalize mitochondria distribution. PC exerts its benefit by inhibiting reactive oxygen species (ROS) production, which decreases apoptosis. Finally, we observe a significant increase in litter size after PC administration to D-galactose-induced aging mice. Our study demonstrates for the first time that D-galactose-induced impaired female reproductive capability can be partially rescued by the antioxidant effects of PC.

show abstract

“…The ratio between the levels of oxidant and antioxidant defenses changes depending on the hormonal conditions. After the gonadotropin surge (human chorionic gonadotropin (hCG) or luteinizing hormone (LH)), ROS levels increase while the expression of antioxidants decrease to enhance ovulation (Laloraya et al 1988, Sato et al 1992, Shkolnik et al 2011. Using in vivo and in vitro assays, it has been shown that antioxidants significantly reduce the LH-induced cumulus mucification/expansion, progesterone production, and activation of two signaling pathways (PKA and MAPK), all events indispensable for the ovulatory process (Shkolnik et al 2011).…”

Section: Introductionmentioning

confidence: 99%

Regulation of the ovarian oxidative status by leptin during the ovulatory process in rats

Bilbao¹,

Yorio²,

Galarza³

et al. 2015

Reproduction

View full text Add to dashboard Cite

Leptin exerts both stimulatory and inhibitory effects on the ovulatory process. In this study, we investigated whether these opposite effects involve changes in the oxidative status in response to different levels of leptin. To this end, we performed both in vivo and in vitro assays using ovaries of immature rats primed with gonadotropins to induce ovulation. Superoxide dismutase (SOD) and catalase (CAT) activity, lipid peroxidation, glutathione (GSH) content, and reactive oxygen species (ROS) were studied as oxidative damage-related parameters. The expression of BCL2, BAX, and caspase 3 were measured by western blot as apoptosis-related biomarkers. The acute treatment with leptin, which inhibits ovulation, decreased SOD activity and increased active caspase 3 expression. No differences were found in CAT activity, lipid peroxidation, or total GSH. In contrast, the daily administration of leptin, which induces ovulation, decreased GSH content, ROS levels, and Bax and active caspase 3 expression, but caused no changes in other parameters. In addition, the daily administration of leptin induced follicular growth, measured by the number of antral follicles in ovarian sections. Using ovarian explant cultures, we found increased BCL2 expression and decreased SOD activity at low and high concentrations of leptin respectively. Thus, leptin can modulate the oxidative status of the ovarian tissue, during the ovulatory process, by acting on different targets according to its circulating levels. At low concentration, leptin seems to play a protective role against the oxidative stress, whereas at high concentrations, this protein seems to be involved in cell death.

show abstract

Dynamic aspects of ovarian superoxide dismutase isozymes during the ovulatory process in the rat

Cited by 68 publications

References 31 publications

Phycocyanin Improves Reproductive Ability in Obese Female Mice by Restoring Ovary and Oocyte Quality

Phycocyanin Improves Reproductive Ability in Obese Female Mice by Restoring Ovary and Oocyte Quality

C-phycocyanin protects against low fertility by inhibiting reactive oxygen species in aging mice

Regulation of the ovarian oxidative status by leptin during the ovulatory process in rats

Contact Info

Product

Resources

About