Dynamic analysis of sugar metabolism in different harvest seasons of pineapple (Ananas comosus L. (Merr.)

Abstract

In pineapple fruits, sugar accumulation plays an important role in flavor characteristics, which varies according to the stage of fruit development. Metabolic changes in the contents of fructose, sucrose and glucose and reducing sugar related to the activities of soluble acid invertase (AI), neutral invertase (NI), sucrose synathase (SS) and sucrose-phosphate synthase (SPS) were studied in winter and summer pineapple fruits in this paper. Sucrose was significantly increased in most of the harvesting winter fruits which reached the peak of 64.87 mg·g-1 FW at 130 days after anthesis, while hexose was mainly accumulated at the 90 day of the summer fruits in July. The ratio of hexose to sucrose was 5.92:0.73 from the winter fruit in February. Interestingly, the activities of SPS and SS synthetic direction of the harvested fruits in February were significantly higher than those in July, whereas the invertase activities were exactly opposite. NI activity showed a similar trend to AI, but the amount of NI activity was higher than AI in both months. Therefore, NI appears to be one of the vital enzymes in pineapple fruit development. Conclusively, the enzyme activities related to sugar play key roles in the eating of quality pineapple, which could be improved by cultivation in different seasons. So we can arbitrate different temperature to improve the quality of pineapple fruits according to market demand.

Investigation of the cause of reduced sugar content in Kiyomi tangor fruit of Ziyang xiangcheng (Citrus junos Sieb. ex Tanaka) rootstock

“…Sucrose, glucose, and fructose are the major nutritional components of fleshy fruits 42 . Many studies found that sucrose, which was transported through sieve elements and then directly enters into the sink organs through the plasmodesmata or apoplastic space, is the main component for sweetness 43,44 . In accordance with the present study results, sugars in most Citrus fruits, including K .…”

“…Sucrose metabolism enzyme was prepared from frozen tissues of K . tangor as described by Zhang 44 . SPS, SS-I, SS-II, AI, and NI activities were assayed using the Plant SPS, SS-I, SS-II, AI, and NI ELISA test kits (Shanghai BOYE Biology Science and Technology Co. Ltd., Shanghai, China) according to the manufacturer’s instructions.…”

Anthocyanin gene enrichment in the distal region of cotton chromosome A07: mechanisms of reproductive organ coloration

“…The determination of anthocyanin content followed the methodology described by Xu et al (2012). Petal samples (0.1 g) were retrieved from a -80°C freezer, ground to a powder in liquid nitrogen, and then mixed with 1 mL of methanol.…”

0.68% of solar-to-hydrogen efficiency and super-high photostability: Panel H2O-to-H2 conversion based on a novel organic-inorganic membrane catalyst

“…16 Lan et al utilized the synergistic effect between polarized electric field and photocarriers to obtain more efficient catalytic overall water splitting performance. 17 Compared with inorganic ferroelectric materials, polyvinylidene fluoride (PVDF), an organic ferroelectric material with high sensitivity of non-ultrasonic and low-frequency vibration, appears higher piezoelectric coefficient (especially the β, γ, δ phases) due to its strong polarity originated from the parallel molecular accumulation. 18 Moreover, in view of the merits of flexibility, chemical inertia, non-toxicity, etc., it is easy to be processed into fabric membrane structure through a simple polymer processing technique to obtain better operability.…”