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Abstract

Background: Vascular thrombosis is an important pathophysiological aspect of sickle cell disease (SCD). This study aimed to detect the inherited risk factors FV Leiden G 1690 A and Prothrombin G 20210 A in sickle cell Anaemia patients. A total of 50blood samples collected from sickle cell disease patients in steady state. The control group consisted of 50 blood samples were collected from healthy people matched with age and gender of study subjects. All samples were investigated for PT, PTT, TT,using Semi automated STA4coagulometer. Level of D-dimer, TAT complex was estimated by ELIZA. Inherited mutations were detected by RFLP method using PCR machine TECHNE (TC-400). The study showed complete absence of FVL mutation (0) % in SCA patients in compare with prothrombin mutation which was found in (18.0%) while the frequency of two mutations in controls is (0%) Comparison of coagulation tests PT, PTT and TT between positive and negative Prothrombin G2021A mutation within the SCD patients, positive patients showed slight prolongation in mean PTT (39.1667s versus 36.6832s in negative patient paralleled with slightly higher in mean level TAT (10.57133 versus 4.94941 ng/ml) and mean level D-dimer (3541. Versus 2261.06 ng/ml). The study concluded that SCA patients exhibit chronic activation coagulation process even in steady state and moreenhanced in heterozygous prothrombin G1962A patients, cohort studies are needed to evaluate the clinical impact of prothrombin mutation on the frequency of vasoclusive crises and other complications.