Dual-probe electrochemical DNA biosensor based on the “Y” junction structure and restriction endonuclease assisted cyclic enzymatic amplification for detection of double-strand DNA of PML/RARα related fusion gene

Wang, Kun; Lei, Yun; Zhong, Guang‐Xian; Zheng, Yufeng; Sun, Zhouliang; Peng, Hua-Ping; Chen, Wei; Liu, Ailin; Chen, Yuanzhong; Lin, Xinhua

doi:10.1016/j.bios.2015.04.071

Cited by 29 publications

(10 citation statements)

References 39 publications

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“…In recent years, with the maturing of microelectronic processing technology, miniaturization has been a trend for electrochemical biosensors, which has laid a foundation for the application of biosensors in on-site quick testing of pathogens of infectious diseases (Cao 2014, Hao et al 2011, Kaewphinit et al 2012, Wang et al 2015. When building immune sensors, nanometer materials are usually adopted for modification of electrodes in order to improve the detection sensitivity.…”

Section: Resultsmentioning

confidence: 99%

Prevention and control of emergent infectious disease with high specific antigen sensor

Zhang

Liu

2017

Artificial Cells, Nanomedicine, and Biotechnology

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This study aims to evaluate the application of a new type of high specificity antigen sensor in detecting the viruses in sudden infectious diseases. Influenza A (H1N1) virus immunosensor was used for the respective determination of the six kinds of antigens of H1N1, H3N2 viral protein, HA protein of H7N9, influenza B virus, adenovirus, and EV71 virus of same dilution degree on the Screen Printed Carbon Electrode (SPCE), so as to test the specificity of the detection method. In addition, various batches of chick embryo allantoic saliva dilution simulation samples were also detected on their recovery (accuracy), repeatability (precision), and stability. The results were as follows: the linear equation was y = 121.33x + 168; the slope of the linear equation was 121.33 nA/HA unit, representing the sensitivity; correlation coefficient was R=0.9921 > 0.90. Using Statistical Analysis System (SAS) software, we found that: the W values of seven sets of data after Shapiro-Wilk detection were 0.853, 0.991, 0.901, 0.906, 0.825, 0.974, and 0.992, respectively; P values were 0.247, 0.831, 0.386, 0.405, 0.174, 0.691, and 0.821, respectively, all of which were greater than 0.05, suggesting that normality was met. The results of homogeneity test for variance were as follows: F = 2.44, P = 0.0775 > 0.05, suggesting that homogeneity of variance was met. The parametric test results were as follows: F = 19114.0, P < 0.0001, suggesting that there were obvious differences between testing data of the seven groups. The determination recovery rate of electrochemical immunosensor was 80-110%. Relative Standard Deviation (RSD) values of repeatability (precision) test of H1N1 influenza virus electrochemical immunosensor were 7.74%, 3.54%, and 2.01%, all of which were smaller than 10%. The signal response of H1N1 electrochemical immune biological sensor could still maintain more than 85% of the original signal within 30 days of storage. In conclusion, H1N1 electrochemical immune biosensor has good specificity and the test results are not affected by other viruses of the same type. Besides, it has good accuracy which can realize the accurate determination of A (H1N1) influenza virus in actual detection. Thus, the requirement of precision measurement of A (H1N1) flu virus detection can be met. Therefore, H1N1 electrochemical immune biosensors can be used in actual detection with good stability.

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Section: Resultsmentioning

confidence: 99%

Prevention and control of emergent infectious disease with high specific antigen sensor

Zhang

Liu

2017

Artificial Cells, Nanomedicine, and Biotechnology

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“…419,420 This endonuclease-assisted target recycling strategy based on branched DNA could be developed to detect disease-related fusion gene or bacteria. 370,421 Some restriction endonucleases worked on specific sequences, which limited the target detection of arbitrary sequences. To overcome the limitation, Wang et al put forward a universal detection strategy for different sequences of nucleic acid based on a restriction endonuclease.…”

Section: Diagnosticsmentioning

confidence: 99%

DNA Functional Materials Assembled from Branched DNA: Design, Synthesis, and Applications

et al. 2020

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DNA is traditionally known as a central genetic biomolecule in living systems. From an alternative perspective, DNA is a versatile molecular building-block for the construction of functional materials, in particular biomaterials, due to its intrinsic biological attributes, molecular recognition capability, sequence programmability, and biocompatibility. The topologies of DNA building-blocks mainly include linear, circular, and branched types. Branched DNA recently has been extensively employed as a versatile building-block to synthesize new biomaterials, and an assortment of promising applications have been explored. In this review, we discuss the progress on DNA functional materials assembled from branched DNA. We first briefly introduce the background information on DNA molecules and sketch the development history of DNA functional materials constructed from branched DNA. In the second part, the synthetic strategies of branched DNA as building-blocks are categorized into base-pairing assembly and chemical bonding. In the third part, construction strategies for the branched DNA-based functional materials are comprehensively summarized including tile-mediated assembly, DNA origami, dynamic assembly, and hybrid assembly. In the fourth part, applications including diagnostics, protein engineering, drug and gene delivery, therapeutics, and cell engineering are demonstrated. In the end, an insight into the challenges and future perspectives is provided. We envision that branched DNA functional materials can not only enrich the DNA nanotechnology by ingenious design and synthesis but also promote the development of interdisciplinary fields in chemistry, biology, medicine, and engineering, ultimately addressing the growing demands on biological and medical-related applications in the real world.

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“…Y-DNA consists of three oligonucleotides that are partially hybridized to each other. Some older biosensors used this feature to perform DNA detections where one DNA stand is fixed to the surface, another DNA stand and target DNA are added to form a specific structure [77].…”

Section: Dna Nanomaterialsmentioning

confidence: 99%

The Novel Nanomaterials Based Biosensors and Their Applications

Şensoy¹,

Muti²

2021

Novel Nanomaterials

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Since the development of the first biosensor reported, biosensor has received considerable attention due to its high selectivity and sensitivity. Biosensors are highly pursued in order to meet the growing demands and challenges in a large number of analytic applications such as medical diagnosis, food safety control, environmental monitoring, or even military defense. Due to the unique physical, chemical, mechanical and electrical properties, nanomaterials have been widely investigated for their ability and used to fabricate sensors. High surface to volume ratio, good stability, excellent electrocatalytic properties of the nanomaterials plays an important role in the sensitive and selective detection of biomolecules. The synthesis of new nanomaterials with different properties is increasingly common in order to improve these counted properties of nanomaterials. This chapter gives an overview of the importance of the development of novel nanomaterials based biosensors technologies. The use of different funtionalized carbon nanomaterilas, metal oxide nanoparticles, metal nanoparticles, polymeric nanoparticles, quantum dots, graphene sheets and other novel nanomaterials in biosensor technology, and their innovations and advantages are discussed.

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Dual-probe electrochemical DNA biosensor based on the “Y” junction structure and restriction endonuclease assisted cyclic enzymatic amplification for detection of double-strand DNA of PML/RARα related fusion gene

Cited by 29 publications

References 39 publications

Prevention and control of emergent infectious disease with high specific antigen sensor

Prevention and control of emergent infectious disease with high specific antigen sensor

DNA Functional Materials Assembled from Branched DNA: Design, Synthesis, and Applications

The Novel Nanomaterials Based Biosensors and Their Applications

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