Dual Insect specific virus infection limits Arbovirus replication in Aedes mosquito cells

Schultz, Michaela J.; Frydman, Horácio M.; Connor, John H.

doi:10.1016/j.virol.2018.03.022

Cited by 93 publications

(90 citation statements)

References 51 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…A previous study explored the effect of PCLPV together with Cell-fusing agent virus (CFAV) on the replication of arboviruses in cell line Aa23 derived from Ae. albopictus [63]. The Aa23 cell lines persistently infected with CFAV was further inoculated with PCLPV.…”

Section: Discussionmentioning

confidence: 99%

The conservation of a core virome inAedesmosquitoes across different developmental stages and continents

Shi

Zhao

Zeng

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

The conservation of a core virome inAedesmosquitoes across different developmental stages and continents

Shi

Zhao

Zeng

et al. 2020

Preprint

View full text Add to dashboard Cite

show abstract

“…Since severe disease from DENV infections can manifest as dengue 4 hemorrhagic fever/dengue shock syndrome [1], DENV establishment in the continental United 5 States is a major concern for public health agencies. In the USA, Florida has experienced 6 increases in local DENV transmission since 2009 [3], driven in part by human and pathogen 7 movement. Ae.…”

Section: Introductionmentioning

confidence: 99%

“…Autochthonous 9 DENV infection occurs sporadically, primarily in Southern Florida with limited local cases 10 elsewhere in the state [3]. 12 13 Recently, reports have indicated that certain insect-specific viruses (ISVs) can negatively 14 impact or enhance arbovirus (including DENV) infections in insect cells [5,6] and mosquitoes 15 [7], respectively. 12 13 Recently, reports have indicated that certain insect-specific viruses (ISVs) can negatively 14 impact or enhance arbovirus (including DENV) infections in insect cells [5,6] and mosquitoes 15 [7], respectively.…”

Section: Introductionmentioning

confidence: 99%

Under-the-radar dengue virus infections in natural populations ofAedes aegyptimosquitoes

Boyles

Mavian

Finol

et al. 2020

Preprint

View full text Add to dashboard Cite

AbstractThe incidence of locally acquired dengue infections increased during the last decade in the United States, compelling a sustained research effort on the dengue mosquito vector, Aedes aegypti, and its microbiome, which has been shown to influence virus transmission success. We examined the ‘metavirome’ of four populations of Ae. aegypti mosquitoes collected in 2016-2017 from Manatee County, Florida. Unexpectedly, we discovered that dengue virus serotype 4 (DENV4) was circulating in these mosquito populations, representing the first documented case of such a phenomenon in the absence of a local DENV4 human case in this county over a two-year period. We confirmed that all of the mosquito populations carried the same DENV4 strain, assembled its full genome, validated infection orthogonally by reverse transcriptase PCR, traced the virus origin, estimated the time period of its introduction to the Caribbean region, as well as explored the viral genetic signatures and mosquito-specific virome associations that potentially mediated DENV4 persistence in mosquitoes. We discuss the significance of prolonged maintenance of these DENV4 infections in Ae. aegypti that occurred in the absence of a DENV4 human index case in Manatee County with respect to the inability of current surveillance paradigms to detect mosquito vector infections prior to a potential local outbreak.ImportanceSince 1999, dengue outbreaks in the continental United States (U.S.) involving local transmission have occurred episodically and only in Florida and Texas. In Florida, these episodes appear to be coincident with increased introductions of dengue virus into the region through human travel and migration from endemic countries. To date, the U.S. public health response to dengue outbreaks is largely reactive, and implementation of comprehensive arbovirus surveillance in advance of predictable transmission seasons, which would enable proactive preventative efforts, remains unsupported. The significance of our finding is that it is the first documented report of non-outbreak DENV4 transmission and maintenance within a local mosquito vector population in the continental U.S.in the absence of a human case during a two-year time period. Our data suggest that molecular surveillance of mosquito populations in high-risk, high tourism areas of the U.S., may allow for proactive, targeted vector control before potential arbovirus outbreaks.

show abstract

“…We identified relatives of Phasi Charoen-like virus (PCLV) in Anopheles from Senegal and Cambodia. PCLV relatives also infect Aedes , where they were observed to reduce the replication of ZIKV and DENV arboviruses [30]. Palm Creek virus, an insect specific flavivirus, causes reduced replication of the West Nile virus and Murray Valley encephalitis arboviruses in Aedes cells [31].…”

Section: Discussionmentioning

confidence: 99%

De novo profiling of RNA viruses inAnophelesmalaria vector mosquitoes from forest ecological zones in Senegal and Cambodia

Nanfack-Minkeu

Eiglmeier

Carissimo

et al. 2018

Preprint

View full text Add to dashboard Cite

BackgroundMosquitoes are colonized by a large but mostly uncharacterized natural virome of RNA viruses. Anopheles mosquitoes are efficient vectors of human malaria, and the composition and distribution of the natural RNA virome may influence the biology and immunity of Anopheles malaria vector populations.ResultsAnopheles vectors of human malaria were sampled in forest village sites in Senegal and Cambodia, including Anopheles funestus, Anopheles gambiae group sp., and Anopheles coustani in Senegal, and Anopheles hyrcanus group sp., Anopheles maculatus group sp., and Anopheles dirus in Cambodia. Small and long RNA sequences were depleted of mosquito host and de novo assembled to yield non-redundant contigs longer than 500 nucleotides. Analysis of the assemblies by sequence similarity to known virus families yielded 125 novel virus sequences, 39 from Senegal Anopheles and 86 from Cambodia. Important monophyletic virus clades in the Bunyavirales and Mononegavirales orders are found in these Anopheles from Africa and Asia. Small RNA size and abundance profiles were used to cluster non-host RNA assemblies that were unclassified by sequence similarity. 39 unclassified non-redundant contigs >500 nucleotides strongly matched a pattern of classic RNAi processing of viral replication intermediates, and 1566 unclassified contigs strongly matched a pattern consistent with piRNAs. Analysis of piRNA expression in Anopheles coluzzii after infection with O’nyong nyong virus (family Togaviridae) suggests that virus infection can specifically alter abundance of some piRNAs.ConclusionsRNA viruses ubiquitously colonize Anopheles vectors of human malaria worldwide. At least some members of the mosquito virome are monophyletic with other arthropod viruses. However, high levels of collinearity and similarity of Anopheles viruses at the peptide level is not necessarily matched by similarity at the nucleotide level, indicating that Anopheles from Africa and Asia are colonized by closely related but clearly diverged virome members. The interplay between small RNA pathways and the virome may represent an important part of the homeostatic mechanism maintaining virome members in a commensal or nonpathogenic state, and host-virome interactions could influence variation in malaria vector competence.

show abstract

Dual Insect specific virus infection limits Arbovirus replication in Aedes mosquito cells

Cited by 93 publications

References 51 publications

The conservation of a core virome inAedesmosquitoes across different developmental stages and continents

The conservation of a core virome inAedesmosquitoes across different developmental stages and continents

Under-the-radar dengue virus infections in natural populations ofAedes aegyptimosquitoes

De novo profiling of RNA viruses inAnophelesmalaria vector mosquitoes from forest ecological zones in Senegal and Cambodia

Contact Info

Product

Resources

About