2020
DOI: 10.3390/plants9121780
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Abstract: Efforts to develop more environmentally friendly alternatives to traditional broad-spectrum pesticides in agriculture have recently turned to RNA interference (RNAi) technology. With the built-in, sequence-specific knockdown of gene targets following delivery of double-stranded RNA (dsRNA), RNAi offers the promise of controlling pests and pathogens without adversely affecting non-target species. Significant advances in the efficacy of this technology have been observed in a wide range of species, including man… Show more

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Cited by 61 publications
(35 citation statements)
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References 142 publications
(172 reference statements)
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“…Nonetheless, these nanoparticle-based RNA pesticides must be simple and the required raw materials should be affordable for low-cost production [61,70]. In addition, application dsRNA-based pesticides through either irrigation or foliar spray must be explored [68] to promote their oral uptake by insects via feeding [15].…”
Section: Dsrna Stability and Efficiencymentioning
confidence: 99%
“…Nonetheless, these nanoparticle-based RNA pesticides must be simple and the required raw materials should be affordable for low-cost production [61,70]. In addition, application dsRNA-based pesticides through either irrigation or foliar spray must be explored [68] to promote their oral uptake by insects via feeding [15].…”
Section: Dsrna Stability and Efficiencymentioning
confidence: 99%
“…SIGS is a technology that promotes silencing by spraying the target dsRNA on the plant surface. Therefore, it is possible to silence a specific phytopathogen gene and protect the plant without the need for plant gene recombination (Cagliari et al, 2019;Wytinck et al, 2020). Hu et al, (2020) 2 5 demonstrated that SIGS targeting P. pachyrhizi genes encoding an acetyl-CoA acyltransferase, a 40S ribosomal protein S16, and glycine cleavage system H protein reduced pustule numbers over 70%.…”
Section: Discussionmentioning
confidence: 99%
“…The mechanisms triggering the sensitivity of different species to RNAi are not fully understood yet. The potential use of RNAi technology for pest and plant pathogen control is influenced by the cellular uptake of dsRNA (Cooper et al, 2019;Wytinck et al, 2020). Starting with the uptake process and followed by the behavior of the dsRNA in the organism there are many steps that will affect RNAi efficiency.…”
Section: Specific Characteristics Of Dsrna-based Pesticidesmentioning
confidence: 99%