DSC study of reversible and irreversible thermal denaturation of concertrated globular protein solutions

Pressure sensitive adhesives based on interpolymer complexes

“…A similar behavior has also been observed for such single-phase systems as partially denaturated proteins and polypeptides. In this case a low-temperature relaxation transition is generally treated as a glass transition, whereas the high-temperature heat capacity jump refers to the processes of thermal denaturation and α-helix -coil transition [91][92][93][94][95][96][97]. In both cases, the existence of two Relaxation properties of PVP -PEG PSAs and their relation to adhesion are described in a series of recent publications [98][99][100][101][102] and briefly discussed in the review [38].…”

“…A c c e p t e d M a n u s c r i p t 94 Thus, domination of the cohesion interaction over free volume space is produced by ladder-like cross-linking of FFP that affects the adhesion properties.…”

Solid state chemistry of proteins IV. what is the meaning of thermal denaturation in freeze dried proteins?

“…The unfolding model is quite general, the main results of the simulations are insensitive to model details, and the experimental unfolding behavior of freeze dried hGH is quite representative of the dry proteins that have been studied. [4][5][6][7][8] …”

“…Since the thermal unfolding in systems that are solid (glassy) at ambient temperature is completely irreversible and does not take place until the temperature far exceeds the glass transition temperature of the system, 1,[3][4][5][6][7][8] it is tempting to conclude that the unfolding event is under complete kinetic control. In fact, even studies with proteins in dilute solution 10 often display highly irreversible unfolding.…”

Protein hydration and glass transitions

“…Other estimates of fragility suggest much stronger behavior (m=30) (Green, Fan and Angell, 1994). In addition, the calorimetric signature of the glass transition in globular proteins, that is the heat capacity jump at T g , is very weak and only becomes obvious on denaturation (Sochava, Belopol'skaya and Smirnova, 1985;Sochava and Smirnova, 1993). The glass transition appears to be smeared out over a very large temperature range.…”