Drug resistance profile of biofilm forming Pseudomonas aeruginosa isolated from aquatic environment in South Eastern Nigeria

Okafor, Collins Onyebuchi Okeke; Ifeanyichukwu, Iroha; Ude, Ibiam Ude; Onuoha, Stanley Chukwudozie; Chika, Ejikeugwu; Ovia, Kenneth Ndidi; Eromonsele, Blessing Osose; Agah, Victor Maduka; Okoronkwo, Chimaobi; Gabriel-Ibeh, I; Okoroafor, Ikechukwu; Nwachukwu, Ogechi Blessing

doi:10.1016/j.envc.2022.100530

Cited by 8 publications

(14 citation statements)

References 43 publications

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“…The CTX-M-1-CTX-M-82 genes and IMP-1/IMP-2 genes were present in Pseudomonas aeruginosa identified in this study (Table 5). Pseudomonas aeruginosa from water sources and nosocomial environment has been reported to show high multi-drug resistance to commonly used antibiotics according to published articles 1,2,3,6,8,9 . This resistance pattern has been traceable to some of the members of Pseudomonas spp., ability to harbour resistance genes and plasmid DNA 1,31,36,37 .…”

Section: Discussionmentioning

confidence: 99%

“…Antimicrobial resistance has risen to all antibiotics introduced into general clinical practice in recent years and is likely to spread to any new antibiotics introduced in the future. The prevalence of antibiotic resistance in a developing country like Nigeria may represent a useful marker to monitor resistance genes because it can provide a good model for ecological studies concerning antimicrobial resistance [1][2][3][4] . The detection of ARGs in drinking water systems in Nigeria is a serious wakeup call on the need for regular surveillance and control measures which have been considered appropriate in other developed countries.…”

Section: Introductionmentioning

confidence: 99%

“…The environmental microbiome is considered to be the natural reservoir of potential antibiotic-resistance genes and has more diversity and novelty than ever expected [8][9][10] . Antibiotic resistance exists in ancient permafrost 1,11,12 , deep terrestrial sub-surfaces 13 , agriculture and animal husbandry and wastewaters 3,6,14 . Since most antibiotics originate from soil microbes, it is obvious that soil is a huge reservoir for antibiotic resistance 8 .…”

Section: Introductionmentioning

confidence: 99%

“…Antibiotic resistance genes are now considered environmental pollution and severe measures to prevent their further spread should be taken 21 . A Better understanding of reservoirs, as well as dissemination of Antibiotic Resistance Genes (ARGs) is needed to curtail the resistance threat in the clinic and the environment 1,22,23 .…”

Section: Introductionmentioning

confidence: 99%

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Antibiotic Resistance Studies of Pseudomonas aeruginosa Harbouring blaCTX-M-1-CTXM-82 and blaIMP-1/IMP-2 Encoding Genes from a Water Treatment Reservoir in South Eastern Nigeria

Okeke¹,

Ude²,

Ndidi³

et al. 2023

J. of Pharmacology and Toxicology

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Background and Objective: The detection of ARGs in drinking water systems in Nigeria is a serious wake-up call on the need for regular surveillance and control measures. This study was carried out to determine the presence of antibiotic-resistance genes in Pseudomonas aeruginosa isolated from the Water Treatment Reservoir in Ebonyi State, Nigeria. Materials and Methods: A total of 70 samples of water were examined in this study. Bacteriological analysis of the test isolates was carried out using standard microbiology techniques. Antibiotic susceptibility studies of the test isolates were determined using the Kirby-Bauer disc diffusion method. Isolates obtained were further identified and characterized by PCR analysis using 16S rRNA gene amplification. Results: A total of 56 (80%) isolates of Pseudomonas spp., were confirmed from 70 water samples tested. The isolates were resistant to imipenem (39.29%), ceftazidime (67.86%), ciprofloxacin (69.64%), ofloxacin (71.43%), ertapenem (83.93%) cefepime (85.71%), ticarcillin (89.29%), amikacin (89.29%), gentamicin (91.93%) and cefotaxime (92.86%) while showing complete resistant to amoxicillin\clavulanic acid, tobramycin, oxacillin and aztreonam (100%). The PCR analysis revealed that bla CTX-M-1-CTX-M-82 was present in Pseudomonas aeruginosa strain F18 16S, Pseudomonas tolaasii strain ATCC 33618 16S, Pseudomonas mendocina strain Y20 16S and Pseudomonas aeruginosa strain NA114 16S while bla IMP-1/IMP-2 gene was present only in Pseudomonas aeruginosa strain F18 16S and Pseudomonas aeruginosa strain NA114 16S. Conclusion: We, report the occurrence of multidrug-resistant Pseudomonas spp., harbouring Extended-Spectrum Beta-Lactamase (ESBL) and carbapenemase resistance genes from a water treatment reservoir in Nigeria.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Antibiotic Resistance Studies of Pseudomonas aeruginosa Harbouring blaCTX-M-1-CTXM-82 and blaIMP-1/IMP-2 Encoding Genes from a Water Treatment Reservoir in South Eastern Nigeria

Okeke¹,

Ude²,

Ndidi³

et al. 2023

J. of Pharmacology and Toxicology

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“…Thus, researchers mediated bacterial QS systems by regulating the concentration of AHL molecules, thereby utilizing QQ as a potential antibacterial strategy (Murugayah and Gerth. 2019;Okafor et al 2022).…”

Section: Introductionmentioning

confidence: 99%

Quorum-quenching potential of recombinant PvdQ-engineered bacteria for biofilm formation

Xie

et al. 2023

Int Microbiol

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Quorum sensing (QS) is a core mechanism for bacteria to regulate bio lm formation, and therefore, QS inhibition or quorum quenching (QQ) is used as an effective and economically feasible strategy against bio lms. In this study, the PvdQ gene encoding AHL acylase was introduced into Escherichia coli (DE3), and a PvdQ-engineered bacterium with highly e cient QQ activity was obtained and used to inhibit bio lm formation. Gene sequencing and western blot analysis showed that the recombinant pET-PvdQ strain was successfully constructed. The color reaction of Agrobacterium tumefaciens A136 indicated that PvdQ engineering bacteria had shown strong AHL signal molecule quenching activity, and signi cantly inhibited the adhesion (motility) of Pseudomonas aeruginosa and bio lm formation of activated sludge bacteria in Membrane Bio-Reactor (MBR; inhibition rate 51-85%, p < 0.05). In addition, qRT-PCR testing revealed that recombinant PvdQ acylase signi cantly reduced the transcription level of QS bio lm formation related genes (cdrA, pqsA, and lasR; p < 0.05). In this study, QQ genetically engineered bacteria enhanced by genetic engineering could effectively inhibit the QS signal transduction mechanism and have the potential to control bio lm formation of pathogenic bacteria in the aquaculture environment, providing an environmentally friendly and alternative antibiotic strategy to suppress bio lm contamination.

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Assessment of antimicrobial resistance, biofilm formation, and surface modification potential in hospital strains of Pseudomonas aeruginosa and Klebsiella pneumoniae

Lordelo,

Branco,

Gama

et al. 2024

Heliyon

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Drug resistance profile of biofilm forming Pseudomonas aeruginosa isolated from aquatic environment in South Eastern Nigeria

Cited by 8 publications

References 43 publications

Antibiotic Resistance Studies of Pseudomonas aeruginosa Harbouring blaCTX-M-1-CTXM-82 and blaIMP-1/IMP-2 Encoding Genes from a Water Treatment Reservoir in South Eastern Nigeria

Antibiotic Resistance Studies of Pseudomonas aeruginosa Harbouring blaCTX-M-1-CTXM-82 and blaIMP-1/IMP-2 Encoding Genes from a Water Treatment Reservoir in South Eastern Nigeria

Quorum-quenching potential of recombinant PvdQ-engineered bacteria for biofilm formation

Assessment of antimicrobial resistance, biofilm formation, and surface modification potential in hospital strains of Pseudomonas aeruginosa and Klebsiella pneumoniae

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