Drug resistance and genotypic analysis of <i>Mycobacterium tuberculosis</i> strains from Thai tuberculosis patients

Cheunoy, Wattana; Haile, Melles; Chaiprasert, Angkana; Prammananan, Therdsak; Cristea-Fernström, Mariana; Vondracek, Martin; Chryssanthou, Erja; Hoffner, Sven; Petrini, Björn

doi:10.1111/j.1600-0463.2009.02438.x

Cited by 8 publications

(7 citation statements)

References 17 publications

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“…Compared to previous studies performed in the South and South-East Asian region (India, Bangladesh, Myanmar, Thailand, Vietnam and this study) we observe that the CAS lineage is totally absent in Cambodia and Vietnam only [6,7,9,14-16]. Indonesia and Malaysia seem to be the lower south-eastern borders of the CAS lineage distribution in South-East Asia.…”

Section: Discussioncontrasting

confidence: 87%

“…With the available data, the hypothesis of MDR-TB transmission could not be rejected. Drug-resistance thus could be associated with Beijing family as already observed in Vietnam, Thailand and South Africa [6,9,18-22]. The exact features of MDR-TB in this region clearly warrant further studies.…”

Section: Discussionmentioning

confidence: 57%

“…Multidrug resistant TB (MDR-TB) is an important aspect of tuberculosis control [8,9]. A high level of resistance to Isoniazid (Inh) and Rifampin (Rif) was identified in Phnom Penh in patients co-infected with HIV, and the minimal number of MDR-TB identified new cases in 2009 in Cambodia was 94 [4].…”

Section: Introductionmentioning

confidence: 99%

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A first assessment of the genetic diversity of Mycobacterium tuberculosis complex in Cambodia

et al. 2011

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BackgroundCambodia is among the 22 high-burden TB countries, and has one of the highest rates of TB in South-East Asia. This study aimed to describe the genetic diversity among clinical Mycobacterium tuberculosis complex (MTC) isolates collected in Cambodia and to relate these findings to genetic diversity data from neighboring countries.MethodsWe characterized by 24 VNTR loci genotyping and spoligotyping 105 Mycobacterium tuberculosis clinical isolates collected between 2007 and 2008 in the region of Phnom-Penh, Cambodia, enriched in multidrug-resistant (MDR) isolates (n = 33).ResultsClassical spoligotyping confirmed that the East-African Indian (EAI) lineage is highly prevalent in this area (60%-68% respectively in whole sample and among non-MDR isolates). Beijing lineage is also largely represented (30% in whole sample, 21% among non-MDR isolates, OR = 4.51, CI95% [1.77, 11.51]) whereas CAS lineage was absent. The 24 loci MIRU-VNTR typing scheme distinguished 90 patterns with only 13 multi-isolates clusters covering 28 isolates. The clustering of EAI strains could be achieved with only 8 VNTR combined with spoligotyping, which could serve as a performing, easy and cheap genotyping standard for this family. Extended spoligotyping suggested relatedness of some unclassified "T1 ancestors" or "Manu" isolates with modern strains and provided finer resolution.ConclusionsThe genetic diversity of MTC in Cambodia is driven by the EAI and the Beijing families. We validate the usefulness of the extended spoligotyping format in combination with 8 VNTR for EAI isolates in this region.

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Section: Discussioncontrasting

confidence: 87%

Section: Discussionmentioning

confidence: 57%

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A first assessment of the genetic diversity of Mycobacterium tuberculosis complex in Cambodia

et al. 2011

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“…Even with the addition of the PTB cases, both the BJ and EAI genotypes, which are classified as SCG2 and SCG1, respectively, were still found to be the endemic genotypes causing TB in Thailand (6,11,12,(34)(35)(36). The prevalences of each genotype did not differ significantly between the PTB and EPTB cases in our study.…”

Section: Discussionmentioning

confidence: 99%

Genetic Diversity and Dynamic Distribution of Mycobacterium tuberculosis Isolates Causing Pulmonary and Extrapulmonary Tuberculosis in Thailand

et al. 2014

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“…Of the 31 studies, 31 studies were evaluated for the mutations of rpoB , 27 studies for the mutations of katG , 18 studies for the mutations of inhA and 9 studies for that of oxyR - ahpC [ 5 , 10 , 15 , 17 , 20 , 21 , 22 , 24 , 43 , 44 , 45 , 46 , 47 , 48 , 49 , 50 , 51 , 52 , 53 , 54 , 55 , 56 , 57 , 58 , 59 , 60 , 61 , 62 , 63 , 64 , 65 , 66 , 67 , 68 , 69 ]. The subgroup analysis was conducted for the mutations of rpoB , katG and inhA instead of the mutations of oxyR-ahpC because only a few studies were included to be analyzed for the latter.…”

Section: Resultsmentioning

confidence: 99%

Comparison on Major Gene Mutations Related to Rifampicin and Isoniazid Resistance between Beijing and Non-Beijing Strains of Mycobacterium tuberculosis: A Systematic Review and Bayesian Meta-Analysis

Guo

Chongsuvivatwong

Lei

2022

Genes

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Objective: The Beijing strain of Mycobacterium tuberculosis (MTB) is controversially presented as the predominant genotype and is more drug resistant to rifampicin and isoniazid compared to the non-Beijing strain. We aimed to compare the major gene mutations related to rifampicin and isoniazid drug resistance between Beijing and non-Beijing genotypes, and to extract the best evidence using the evidence-based methods for improving the service of TB control programs based on genetics of MTB. Method: Literature was searched in Google Scholar, PubMed and CNKI Database. Data analysis was conducted in R software. The conventional and Bayesian random-effects models were employed for meta-analysis, combining the examinations of publication bias and sensitivity. Results: Of the 8785 strains in the pooled studies, 5225 were identified as Beijing strains and 3560 as non-Beijing strains. The maximum and minimum strain sizes were 876 and 55, respectively. The mutations prevalence of rpoB, katG, inhA and oxyR-ahpC in Beijing strains was 52.40% (2738/5225), 57.88% (2781/4805), 12.75% (454/3562) and 6.26% (108/1724), respectively, and that in non-Beijing strains was 26.12% (930/3560), 28.65% (834/2911), 10.67% (157/1472) and 7.21% (33/458), separately. The pooled posterior value of OR for the mutations of rpoB was 2.72 ((95% confidence interval (CI): 1.90, 3.94) times higher in Beijing than in non-Beijing strains. That value for katG was 3.22 (95% CI: 2.12, 4.90) times. The estimate for inhA was 1.41 (95% CI: 0.97, 2.08) times higher in the non-Beijing than in Beijing strains. That for oxyR-ahpC was 1.46 (95% CI: 0.87, 2.48) times. The principal patterns of the variants for the mutations of the four genes were rpoB S531L, katG S315T, inhA-15C > T and oxyR-ahpC intergenic region. Conclusion: The mutations in rpoB and katG genes in Beijing are significantly more common than that in non-Beijing strains of MTB. We do not have sufficient evidence to support that the prevalence of mutations of inhA and oxyR-ahpC is higher in non-Beijing than in Beijing strains, which provides a reference basis for clinical medication selection.

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Drug resistance and genotypic analysis of Mycobacterium tuberculosis strains from Thai tuberculosis patients

Cited by 8 publications

References 17 publications

A first assessment of the genetic diversity of Mycobacterium tuberculosis complex in Cambodia

A first assessment of the genetic diversity of Mycobacterium tuberculosis complex in Cambodia

Genetic Diversity and Dynamic Distribution of Mycobacterium tuberculosis Isolates Causing Pulmonary and Extrapulmonary Tuberculosis in Thailand

Comparison on Major Gene Mutations Related to Rifampicin and Isoniazid Resistance between Beijing and Non-Beijing Strains of Mycobacterium tuberculosis: A Systematic Review and Bayesian Meta-Analysis

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