Drk-mediated signaling to Rho kinase is required for anesthesia-resistant memory in
            <i>Drosophila</i>

Kotoula, Vasileia; Moressis, Anastasios; Semelidou, Ourania; Skoulakis, Efthimios M. C.

doi:10.1073/pnas.1704835114

Cited by 14 publications

(29 citation statements)

References 41 publications

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“…The interaction of RADISH and BRUCHPILOT at the synaptic active zone has been proposed to regulate neurotransmitter release[17], and genetic knockdown of radish or bruchpilot in αβ neurons disrupts ARM[17, 20]. A recent study indicated that Drk–Drok signaling is essential for ARM formation in αβ neurons, and related to dynamic cytoskeletal changes[33]. In addition, the dopamine type 2 (D2R) and serotonin (5HT1A) receptors in αβ neurons are also critical for ARM formation[34, 35].…”

Section: Discussionmentioning

confidence: 99%

Electrical synapses between mushroom body neurons are critical for consolidated memory retrieval in Drosophila

Shyu¹,

Lee²,

Chiang³

et al. 2019

PLoS Genet

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Electrical synapses between neurons, also known as gap junctions, are direct cell membrane channels between adjacent neurons. Gap junctions play a role in the synchronization of neuronal network activity; however, their involvement in cognition has not been well characterized. Three-hour olfactory associative memory in Drosophila has two components: consolidated anesthesia-resistant memory (ARM) and labile anesthesia-sensitive memory (ASM). Here, we show that knockdown of the gap junction gene innexin5 (inx5) in mushroom body (MB) neurons disrupted ARM, while leaving ASM intact. Whole-mount brain immunohistochemistry indicated that INX5 protein was preferentially expressed in the somas, calyxes, and lobes regions of the MB neurons. Adult-stage-specific knockdown of inx5 in αβ neurons disrupted ARM, suggesting a specific requirement of INX5 in αβ neurons for ARM formation. Hyperpolarization of αβ neurons during memory retrieval by expressing an engineered halorhodopsin (eNpHR) also disrupted ARM. Administration of the gap junction blocker carbenoxolone (CBX) reduced the proportion of odor responsive αβ neurons to the training odor 3 hours after training. Finally, the α-branch-specific 3-hour ARM-specific memory trace was also diminished with CBX treatment and in inx5 knockdown flies. Altogether, our results suggest INX5 gap junction channels in αβ neurons for ARM retrieval and also provide a more detailed neuronal mechanism for consolidated memory in Drosophila .

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Section: Discussionmentioning

confidence: 99%

Electrical synapses between mushroom body neurons are critical for consolidated memory retrieval in Drosophila

Shyu¹,

Lee²,

Chiang³

et al. 2019

PLoS Genet

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“…The MEF2 MB lobe expression pattern expression gives clues to possible transcriptional interactors for MEF2. Examples of MB markers with similar Kenyon cell subtype distribution to MEF2 include FOXP ( DasGupta et al, 2014 ), HDAC4 ( Fitzsimons et al, 2013 ), DRK ( Crittenden et al, 1998 ; Kotoula et al, 2017 ), and FASII ( Crittenden et al, 1998 ; Cheng et al, 2001 ). MEF2 interactions with several of these molecules have already been established.…”

Section: Discussionmentioning

confidence: 99%

Drosophila mef2is essential for normal mushroom body and wing development

et al. 2018

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MEF2 (myocyte enhancer factor 2) transcription factors are found in the brain and muscle of insects and vertebrates and are essential for the differentiation of multiple cell types. We show that in the fruit fly Drosophila, MEF2 is essential for the formation of mushroom bodies in the embryonic brain and for the normal development of wings in the adult. In embryos mutant for mef2, there is a striking reduction in the number of mushroom body neurons and their axon bundles are not detectable. The onset of MEF2 expression in neurons of the mushroom bodies coincides with their formation in the embryo and, in larvae, expression is restricted to post-mitotic neurons. In flies with a mef2 point mutation that disrupts nuclear localization, we find that MEF2 is restricted to a subset of Kenyon cells that project to the α/β, and γ axonal lobes of the mushroom bodies, but not to those forming the α’/β’ lobes.

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“…Flies were dissected in cold PBS, fixed in 4% paraformaldehyde for 15 min, and imaged by laser-scanning confocal microscopy (TCS SP8, Leica). F-actin levels were determined in adult fly brains from three independent experiments as described previously (Fulga et al, 2007;Kotoula et al, 2017).…”

Section: Methodsmentioning

confidence: 99%

DrosophilaTau Negatively Regulates Translation and Olfactory Long-Term Memory, But Facilitates Footshock Habituation and Cytoskeletal Homeostasis

Papanikolopoulou¹,

Roussou²,

Gouzi³

et al. 2019

J. Neurosci.

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Although the involvement of pathological tau in neurodegenerative dementias is indisputable, its physiological roles have remained elusive in part because its abrogation has been reported without overt phenotypes in mice and Drosophila. This was addressed using the recently described Drosophila tau KO and Mi{MIC} mutants and focused on molecular and behavioral analyses. Initially, we show that Drosophila tau (dTau) loss precipitates dynamic cytoskeletal changes in the adult Drosophila CNS and translation upregulation. Significantly, we demonstrate for the first time distinct roles for dTau in adult mushroom body (MB)-dependent neuroplasticity as its downregulation within ␣Ј␤Јneurons impairs habituation. In accord with its negative regulation of translation, dTau loss specifically enhances protein synthesis-dependent long-term memory (PSD-LTM), but not anesthesia-resistant memory. In contrast, elevation of the protein in the MBs yielded premature habituation and depressed PSD-LTM. Therefore, tau loss in Drosophila dynamically alters brain cytoskeletal dynamics and profoundly affects neuronal proteostasis and plasticity.

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Drk-mediated signaling to Rho kinase is required for anesthesia-resistant memory in Drosophila

Cited by 14 publications

References 41 publications

Electrical synapses between mushroom body neurons are critical for consolidated memory retrieval in Drosophila

Electrical synapses between mushroom body neurons are critical for consolidated memory retrieval in Drosophila

Drosophila mef2is essential for normal mushroom body and wing development

DrosophilaTau Negatively Regulates Translation and Olfactory Long-Term Memory, But Facilitates Footshock Habituation and Cytoskeletal Homeostasis

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