Driving forces for the uphill transport of amino acids into epidermal brush border membrane vesicles of the sea anemone, Anemonia sulcata (Cnidaria, anthozoa)

Uptake of lysine and prolinevia separate α-neutral amino acid transport pathways inMytilus gill brush border membranes

“…For example, the rate of Na +-supported lysine transport in BBMV from the lepidopteran, Philosomia cynthia, midgut is 67% of that seen in K + (Giordana et al, 1985). The results of a recent study suggest that this phenomenon may exist in another marine invertebrate, the sea anemone, Anemonia sulcata (Buck & Schlichter, 1987). These authors reported "overshoots" for leucine transport in sea anemone BBMV in the presence of inwardly directed KC1 gradients.…”

Uptake of lysine and prolinevia separate α-neutral amino acid transport pathways inMytilus gill brush border membranes

“…For example, the rate of Na +-supported lysine transport in BBMV from the lepidopteran, Philosomia cynthia, midgut is 67% of that seen in K + (Giordana et al, 1985). The results of a recent study suggest that this phenomenon may exist in another marine invertebrate, the sea anemone, Anemonia sulcata (Buck & Schlichter, 1987). These authors reported "overshoots" for leucine transport in sea anemone BBMV in the presence of inwardly directed KC1 gradients.…”

Involvement of H+-ATPase and carbonic anhydrase in inorganic carbon uptake for endosymbiont photosynthesis

“…2-5 legends. All resuspension buffers contained valinomycin, an ionophore shown to be effective on plasma membrane of A. viridis (15). Stock solution of valinomycin was prepared in ethanol (2.5 mM).…”

Nutrient Absorption in Invertebrates

“…In both groups, inwardly directed gradients of Na + were capable of supporting concentrative accumulation of amino acids (83, 320, 321, 383). Significantly, Na + was not the only cation capable of serving as an energetic activator of transport; in coelenterate brush‐border membrane vesicles, uphill transport was also supported by inwardly directed gradients of K + and choline (83), while in brush‐border membrane vesicles from bivalve gill, gradients of K + and Li + drove uphill transport (321). In both groups, however, activation of transport was greater in the presence of an Na + gradient than with gradients of other cations.…”

“…This effectively limits the use of experimental protocols using isolated membranes to studies in which individual animals are comparatively large or where large numbers of moderately sized individuals can be conveniently collected and dissected rapidly. Despite this significant limitation, studies using epithelial membranes isolated from molluscs (319, 320, 321), coelenterates (83, 383, 395), crustaceans (5), and echinoderms (7, 9) have proven valuable in the identification and detailed study of several unique transport processes discussed in upcoming sections of this chapter.…”

“…Their results revealed many similarities between integumental transport in coelenterates and bivalve molluscs. In both groups, inwardly directed gradients of Na + were capable of supporting concentrative accumulation of amino acids (83, 320, 321, 383). Significantly, Na + was not the only cation capable of serving as an energetic activator of transport; in coelenterate brush‐border membrane vesicles, uphill transport was also supported by inwardly directed gradients of K + and choline (83), while in brush‐border membrane vesicles from bivalve gill, gradients of K + and Li + drove uphill transport (321).…”

Uptake of lysine and prolinevia separate α-neutral amino acid transport pathways inMytilus gill brush border membranes

“…For example, the rate of Na +-supported lysine transport in BBMV from the lepidopteran, Philosomia cynthia, midgut is 67% of that seen in K + (Giordana et al, 1985). The results of a recent study suggest that this phenomenon may exist in another marine invertebrate, the sea anemone, Anemonia sulcata (Buck & Schlichter, 1987). These authors reported "overshoots" for leucine transport in sea anemone BBMV in the presence of inwardly directed KC1 gradients.…”

Involvement of H+-ATPase and carbonic anhydrase in inorganic carbon uptake for endosymbiont photosynthesis

“…2-5 legends. All resuspension buffers contained valinomycin, an ionophore shown to be effective on plasma membrane of A. viridis (15). Stock solution of valinomycin was prepared in ethanol (2.5 mM).…”

Nutrient Absorption in Invertebrates

“…In both groups, inwardly directed gradients of Na + were capable of supporting concentrative accumulation of amino acids (83, 320, 321, 383). Significantly, Na + was not the only cation capable of serving as an energetic activator of transport; in coelenterate brush‐border membrane vesicles, uphill transport was also supported by inwardly directed gradients of K + and choline (83), while in brush‐border membrane vesicles from bivalve gill, gradients of K + and Li + drove uphill transport (321). In both groups, however, activation of transport was greater in the presence of an Na + gradient than with gradients of other cations.…”

“…This effectively limits the use of experimental protocols using isolated membranes to studies in which individual animals are comparatively large or where large numbers of moderately sized individuals can be conveniently collected and dissected rapidly. Despite this significant limitation, studies using epithelial membranes isolated from molluscs (319, 320, 321), coelenterates (83, 383, 395), crustaceans (5), and echinoderms (7, 9) have proven valuable in the identification and detailed study of several unique transport processes discussed in upcoming sections of this chapter.…”

“…Their results revealed many similarities between integumental transport in coelenterates and bivalve molluscs. In both groups, inwardly directed gradients of Na + were capable of supporting concentrative accumulation of amino acids (83, 320, 321, 383). Significantly, Na + was not the only cation capable of serving as an energetic activator of transport; in coelenterate brush‐border membrane vesicles, uphill transport was also supported by inwardly directed gradients of K + and choline (83), while in brush‐border membrane vesicles from bivalve gill, gradients of K + and Li + drove uphill transport (321).…”

Uptake of lysine and prolinevia separate α-neutral amino acid transport pathways inMytilus gill brush border membranes

“…For example, the rate of Na +-supported lysine transport in BBMV from the lepidopteran, Philosomia cynthia, midgut is 67% of that seen in K + (Giordana et al, 1985). The results of a recent study suggest that this phenomenon may exist in another marine invertebrate, the sea anemone, Anemonia sulcata (Buck & Schlichter, 1987). These authors reported "overshoots" for leucine transport in sea anemone BBMV in the presence of inwardly directed KC1 gradients.…”

Involvement of H+-ATPase and carbonic anhydrase in inorganic carbon uptake for endosymbiont photosynthesis

“…2-5 legends. All resuspension buffers contained valinomycin, an ionophore shown to be effective on plasma membrane of A. viridis (15). Stock solution of valinomycin was prepared in ethanol (2.5 mM).…”

Nutrient Absorption in Invertebrates

“…In both groups, inwardly directed gradients of Na + were capable of supporting concentrative accumulation of amino acids (83, 320, 321, 383). Significantly, Na + was not the only cation capable of serving as an energetic activator of transport; in coelenterate brush‐border membrane vesicles, uphill transport was also supported by inwardly directed gradients of K + and choline (83), while in brush‐border membrane vesicles from bivalve gill, gradients of K + and Li + drove uphill transport (321). In both groups, however, activation of transport was greater in the presence of an Na + gradient than with gradients of other cations.…”

“…This effectively limits the use of experimental protocols using isolated membranes to studies in which individual animals are comparatively large or where large numbers of moderately sized individuals can be conveniently collected and dissected rapidly. Despite this significant limitation, studies using epithelial membranes isolated from molluscs (319, 320, 321), coelenterates (83, 383, 395), crustaceans (5), and echinoderms (7, 9) have proven valuable in the identification and detailed study of several unique transport processes discussed in upcoming sections of this chapter.…”

“…Their results revealed many similarities between integumental transport in coelenterates and bivalve molluscs. In both groups, inwardly directed gradients of Na + were capable of supporting concentrative accumulation of amino acids (83, 320, 321, 383). Significantly, Na + was not the only cation capable of serving as an energetic activator of transport; in coelenterate brush‐border membrane vesicles, uphill transport was also supported by inwardly directed gradients of K + and choline (83), while in brush‐border membrane vesicles from bivalve gill, gradients of K + and Li + drove uphill transport (321).…”