Draft Genome Sequence of New Bacillus cereus Strain tsu1

Abstract

This paper reports the draft genome sequence of a new Bacillus cereus strain tsu1, isolated on an agar-cellulose plate. The draft genome sequence is 5.81 Mb, revealing 5,673 coding sequences. It contains genes for cellulose-degradation and biosynthesis pathways of polyhydroxybutyrate (PHB) and 8 rRNA genes (5S, 16S, and 23S).

Genome Structure ofBacillus cereustsu1 and Genes Involved in Cellulose Degradation and Poly-3-Hydroxybutyrate Synthesis

“…Previously, we reported the genome sequence of B. cereus tsu1 [27]. This paper reports on the assembly of the genomic structure, and characterization of cellulolytic and PHB producing activities of this strain.…”

“…In previous research, draft genome of Bacillus cereus tsu1 was generated using next generation sequencing analysis [27]. To generate an alignment map of the assembled tsu1 scaffolds (GenBank: KN321896-KN321931) using MUMmer (version 3.0) on the galaxy working station (biou.psc.edu/galaxy) [28], the genome sequence of B. cereus B4264 (NCBI GenBank: CP001176.1) with the highest identity similarity clustered by phylogenetic COG (PCOGR) was downloaded from NCBI database and used as the reference ( Figure S1 in Supplementary Material, available online at https://doi.org/10.1155/2017/6192924) [29].…”

“…Protein pellets were air-dried, solubilized in a 1x Laemmli protein sample buffer (Biorad), and denatured by boiling for 5 min. Proteins were separated on a SDS-PAGE protein gel [27]. After staining with Coomassie Blue, protein bands were isolated from the gel and digested with trypsin [32], followed by liquid chromatography (LC)/mass spectrometry (MS) as described above.…”

“…The 36 assembled scaffolds of B. cereus tsu1 were each aligned to a distinct region of the genome from B. cereus B4262 [27]. Regions of scaffolds 4, 6, and 15 did not match the reference genome ( Figure 1).…”

“…Among all the proteins identified by searching the tryptic digestion peptides against the B. cereus tsu1 protein dataset annotated by prokaryotic genome annotation pipeline, one endo-glucanase matching (GenBank ID: KGT43479.1) was identified (Table S5 in the Supplementary Material) [27].…”