Draft Genome Sequence of an Anaerobic Ammonium-Oxidizing Bacterium, “
            <i>Candidatus</i>
            Brocadia sinica”

Oshiki, Mamoru; Shinyako-Hata, Kaori; Satoh, Hisashi; Okabe, Satoshi

doi:10.1128/genomea.00267-15

Cited by 82 publications

(44 citation statements)

References 13 publications

(10 reference statements)

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“…In the present study, we showed the intracellular localization of the hydroxylamine oxidoreductase (HAO)-like protein kustc0457/58, hydrazine dehydrogenase (HDH), the NO-producing HAO-like protein HOX, nitrite oxidoreductase (NXR), and hydrazine synthase (HZS) within an anammox cell. All of these proteins are encoded in all presently available anammox genomes and play key roles in anammox catabolism (1,2,(9)(10)(11)(12)(13)(14). The fact that all studied protein complexes were exclusively immunolocalized to the anammoxosome in the present study supported the hypothesis that this cell compartment was a true prokaryotic organelle with a dedicated function.…”

Section: Discussionsupporting

confidence: 76%

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Immunogold Localization of Key Metabolic Enzymes in the Anammoxosome and on the Tubule-Like Structures of Kuenenia stuttgartiensis

et al. 2015

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Anaerobic ammonium-oxidizing (anammox) bacteria oxidize ammonium with nitrite as the terminal electron acceptor to form dinitrogen gas in the absence of oxygen. Anammox bacteria have a compartmentalized cell plan with a central membrane-bound "prokaryotic organelle" called the anammoxosome. The anammoxosome occupies most of the cell volume, has a curved membrane, and contains conspicuous tubule-like structures of unknown identity and function. It was suggested previously that the catalytic reactions of the anammox pathway occur in the anammoxosome, and that proton motive force was established across its membrane. Here, we used antibodies raised against five key enzymes of the anammox catabolism to determine their cellular location. The antibodies were raised against purified native hydroxylamine oxidoreductase-like protein kustc0458 with its redox partner kustc0457, hydrazine dehydrogenase (HDH; kustc0694), hydroxylamine oxidase (HOX; kustc1061), nitrite oxidoreductase (NXR; kustd1700/03/04), and hydrazine synthase (HZS; kuste2859-61) of the anammox bacterium Kuenenia stuttgartiensis. We determined that all five protein complexes were exclusively located inside the anammoxosome matrix. Four of the protein complexes did not appear to form higher-order protein organizations. However, the present data indicated for the first time that NXR is part of the tubule-like structures, which may stretch the whole length of the anammoxosome. These findings support the anammoxosome as the locus of catabolic reactions of the anammox pathway. IMPORTANCEAnammox bacteria are environmentally relevant microorganisms that contribute significantly to the release of fixed nitrogen in nature. Furthermore, the anammox process is applied for nitrogen removal from wastewater as an environment-friendly and cost-effective technology. These microorganisms feature a unique cellular organelle, the anammoxosome, which was proposed to contain the energy metabolism of the cell and tubule-like structures with hitherto unknown function. Here, we purified five native enzymes catalyzing key reactions in the anammox metabolism and raised antibodies against these in order to localize them within the cell. We showed that all enzymes were located within the anammoxosome, and nitrite oxidoreductase was located exclusively at the tubule-like structures, providing the first insights into the function of these subcellular structures.

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Section: Discussionsupporting

confidence: 76%

“…Apart from nitrite reductase, all currently available anammox genomes encode close homologs of the proteins described above (2,(9)(10)(11)(12)(13)(14), suggesting that they have the same energy metabolism as K. stuttgartiensis.…”

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confidence: 99%

Immunogold Localization of Key Metabolic Enzymes in the Anammoxosome and on the Tubule-Like Structures of Kuenenia stuttgartiensis

et al. 2015

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“…This implies a more diverse cellular metabolism in the IFAS sludges. Of the genome assemblies of Candidatus Brocadia detected in the sludges, these samples had had very low coverage rates (4.7∼7.2%) to the reference genome Candidatus ‘Brocadia sinica’ (Oshiki et al ., ) (http://onlinelibrary.wiley.com/doi/10.1111/1462-2920.13132/suppinfo).…”

Section: Resultsmentioning

confidence: 99%

Metagenomic analysis of anammox communities in three different microbial aggregates

Guo

Peng

Lü

et al. 2016

Environmental Microbiology

147

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There is great potential to understand the functional diversity of microorganisms that are involved in waste water treatment through metagenomic analyses. This study presents the first metagenomic comparison of taxonomic and functional profiles of the microbial communities occurring in different aggregates from anaerobic ammonium-oxidizing (anammox) bioreactors. The anammox bacterial communities in both biofilm and granule sludge samples showed relatively high abundance and diversity compared with floccular sludge. Four of the five known genera of anammox bacteria were detected in the three cultures except Candidatus Jettenia, which was absent in the granules. Candidatus Kuenenia comprised the major population of anammox bacteria in these three sludges, independent of their growth morphologies. The genome assembled for the Candidatus Kuenenia in the granule was very similar to the published reference genome of Candidatus K. stuttgartiensis. Genes involved in the metabolism of the anammox process were highly detected in the biofilm and granule sludges. In particular, the abundance of hydrazine synthase gene (hzs) in the biofilm was around 486 times more pronounced than that in the granules. The knowledge gained in this study highlights an important role of sludge aggregate in affecting community structure and metabolic potential of anammox systems.

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“…Kuenenia’ (Strous et al ., ; Speth et al ., ), ‘ Ca . Brocadia’ (Gori et al ., ; Oshiki et al ., ), ‘ Ca . Jettenia’ (Hira et al ., ; Hu et al ., ) and ‘ Ca .…”

Section: Physiology Of Anammox Bacteriamentioning

confidence: 99%

Ecology and physiology of anaerobic ammonium oxidizing bacteria

Oshiki

Satoh

Okabe

2016

Environmental Microbiology

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332

148

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Summary Anaerobic ammonium oxidation (anammox) is a microbial process in which NH4+ is oxidized to N2 gas with NO2− as an electron acceptor. The anammox process is mediated by bacterial members affiliated with the phylum Planctomycetes, which are ubiquitously detected from anoxic natural and man-made ecosystems and a key player in the global nitrogen cycle. In the past two decades, phylogenetically different anammox bacteria have been recognized in natural and synthetic ecosystems (i.e. 'Candidatus Kuenenia', 'Candidatus Brocadia', 'Candidatus Jettenia', 'Candidatus Anammoxoglobus' and 'Candidatus Scalindua' genera), and the geographic distributions of these anammox bacteria indicate that they have genus-specific or species-specific habitats. Recently, we revealed the physiological characteristics of 'Ca. Jettenia' in addition to 'Ca. Kuenenia', 'Ca. Brocadia' and 'Ca. Scalindua', and, as a result, it is possible to compare the physiological characteristics of the anammox bacteria and discuss their niche partitioning. Therefore, we summarize the current knowledge of anammox bacterial ecology and physiology in this review to assess the potential ecological niche partitioning of anammox bacteria in natural and synthetic ecosystems.

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Draft Genome Sequence of an Anaerobic Ammonium-Oxidizing Bacterium, “ Candidatus Brocadia sinica”

Cited by 82 publications

References 13 publications

Immunogold Localization of Key Metabolic Enzymes in the Anammoxosome and on the Tubule-Like Structures of Kuenenia stuttgartiensis

Immunogold Localization of Key Metabolic Enzymes in the Anammoxosome and on the Tubule-Like Structures of Kuenenia stuttgartiensis

Metagenomic analysis of anammox communities in three different microbial aggregates

Ecology and physiology of anaerobic ammonium oxidizing bacteria

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