Downregulation of SLC9A8 Promotes Epithelial–Mesenchymal Transition and Metastasis in Colorectal Cancer Cells via the IL6-JAK1/STAT3 Signaling Pathway

Liu, Wei; Geng, Chong; Li, Xiao; Li, Yanni; Song, Shuailing; Wang, C

doi:10.1007/s10620-022-07805-0

Cited by 6 publications

(1 citation statement)

References 30 publications

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“…In a study on colorectal cancer, Teppei Morikawa and others analyzed the p-STAT3 immunohistochemical staining results of 724 colorectal cancer pathologies and found that p-STAT3 was significantly associated with poor prognosis in colorectal cancer patients [ 29 ]. Furthermore, the activation of JAK1-STAT3-related signals plays a significant role in the metastasis of colorectal cancer [ 30 ]. These studies have all demonstrated the important role of the STAT3 pathway in the proliferation and migration of colorectal cancer.…”

Section: Discussionmentioning

confidence: 99%

CEBPB-mediated upregulation of SERPINA1 promotes colorectal cancer progression by enhancing STAT3 signaling

Ma,

Chen,

Zhan

et al. 2024

Cell Death Discov.

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Colorectal cancer (CRC) is a highly malignant carcinoma associated with poor prognosis, and metastasis is one of the most common causes of death in CRC. Serpin Family A Member 1 (SERPINA1) is a serine protease inhibitor from the Serpin family. Till now, the function and mechanism of SERPINA1 in CRC progression have not been fully illustrated. We established highly metastatic colorectal cancer cells named as RKO-H and Caco2-H by mice liver metastasis model. By integrative bioinformatic approaches, we analyzed the prognostic value and clinical significance of SERPINA1 in CRC, and predicted potential transcription factors. Colony formation, EDU, MTS, Transwell and wound healing assay were performed to evaluate the biological functions of SERPINA1 in CRC in vitro. Experiments in vivo were conducted to explore the effects of SERPINA1 on liver metastasis of CRC. ChIP and luciferase reporter gene assays were performed to identify the transcriptional regulatory mechanism of SERPINA1 by CEBPB. Our results show that SERPINA1 is highly expressed in CRC and correlated with poor clinical outcomes. SERPINA1 promotes the proliferation, migration by activating STAT3 pathway. Mechanistically, CEBPB binds SERPINA1 gene promoter sequence and promotes the transcription of SERPINA1. SERPINA1 drives CEBPB-induced tumor cell growth and migration via augmenting STAT3 signaling. Our results suggest that SERPINA1 is a potential prognostic marker and may serve as a novel treatment target for CRC.

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Section: Discussionmentioning

confidence: 99%