Downregulation of Ribosomal Contents and Kinase Activities Is Associated with the Inhibitive Effect on the Growth of Group B Streptococcus Induced by Placental Extracellular Vesicles

Gao, Jing; Tang, Yiqun; Sun, Xiance; Chen, Qiujing; Peng, Yiqian; Tsai, Catherine Jia-Yun; Chen, Qi

doi:10.3390/biology10070664

Cited by 1 publication

(2 citation statements)

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“…Upregulation of gene transcription of various ribosomal proteins was noted when Clostridium difficile was treated with clindamycin and amoxicillin and when Streptococcus pneumoniae R6 was treated with erythromycin, chloramphenicol, tetracycline, and puromycin. , On the other hand, 30S ribosomal protein S13, responsible for RNA/tRNA binding, was downregulated as an effect of both JR and MA extracts. The downregulation of this protein was associated with an antimicrobial effect of placental extracellular vesicles on group B Streptococcus …”

Section: Discussionmentioning

confidence: 99%

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Proteomic Profiling Reveals Cytotoxic Mechanisms of Action and Adaptive Mechanisms of Resistance in Porphyromonas gingivalis: Treatment with Juglans regia and Melaleuca alternifolia

et al. 2023

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The increasing trend in the rise of antibiotic-resistant bacteria pushes research to discover new efficacious antibacterial agents from natural and synthetic sources. Porphyromonas gingivalis is a well-known bacterium commonly known for causing periodontal disease, and it is associated with the pathogenesis of life-changing systemic conditions such as Alzheimer’s. Proteomic research can be utilized to test new antibacterial drugs and understand the adaptive resistive mechanisms of bacteria; hence, it is important in the drug discovery process. The current study focuses on identifying the antibacterial effects of Juglans regia (JR) and Melaleuca alternifolia (MA) on P. gingivalis and uses proteomics to identify modes of action while exploring its adaptive mechanisms. JR and MA extracts were tested for antibacterial efficacy using the agar well diffusion assay. A proteomic study was conducted identifying upregulated and downregulated proteins compared to control by 2D-DIGE analysis, and proteins were identified using MADLI-TOF/MS. The bacterial inhibition for JR was 20.14 ± 0.2, and that for MA was 19.72 ± 0.5 mm. Out of 88 differentially expressed proteins, there were 17 common differentially expressed proteins: 10 were upregulated and 7 were downregulated in both treatments. Among the upregulated proteins were Arginine-tRNA ligase, ATP-dependent Clp protease proteolytic, and flavodoxins. In contrast, down-regulated proteins were ATP synthase subunit alpha and quinone, among others, which are known antibacterial targets. STRING analysis indicated a strong network of interactions between differentially expressed proteins, mainly involved in protein translation, post-translational modification, energy production, metabolic pathways, and protein repair and degradation. Both extracts were equi-efficacious at inhibiting P. gingivalis and displayed some overlapping proteomic profiles. However, the MR extract had a greater fold change in its profile than the JA extract. Downregulated proteins indicated similarity in the mode of action, and upregulated proteins appear to be related to adaptive mechanisms important in promoting repair, growth, survival, virulence, and resistance. Hence, both extracts may be useful in preventing P. gingivalis-associated conditions. Furthermore, our results may be helpful to researchers in identifying new antibiotics which may offset these mechanisms of resistance.

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Section: Discussionmentioning

confidence: 99%

“…The downregulation of this protein was associated with an antimicrobial effect of placental extracellular vesicles on group B Streptococcus. 35 4.3. Arginine-tRNA Ligase.…”

Section: D-dige Assessment and Proteomic Studymentioning

confidence: 99%