Downregulation of microRNA-145 is associated with aggressive progression and poor prognosis in human cervical cancer

Abstract

MicroRNAs (miRNAs) play important roles in the processes of tumor initiation and progression. However, miR-145 expression in cervical cancer has been rarely investigated. The aim of this study was to investigate the clinical significance and prognostic value of miR-145 expression in cervical cancer. MiR-145 expression in 114 pairs of human cervical cancer and adjacent normal tissues was detected by real-time quantitative RT-PCR assay. The results showed that miR-145 expression was significantly downregulated in cervical cancer tissues when compared with corresponding adjacent normal tissues (P < 0.001). It was also significantly lower in the cancerous tissues of patients with advanced International Federation of Gynecology and Obstetrics (FIGO) stage cervical cancer than those with early FIGO stage (P = 0.006). In addition, miR-145 was expressed at significantly lower levels in lymph node metastasis-positive patients than in lymph node metastasis-negative patients (P = 0.037). Moreover, poorly differentiated tumors expressed lower miR-145 than well or moderately differentiated tumors (P = 0.012). Patients with vascular invasion or human papillomavirus (HPV) infection also had lower miR-145 expression levels than those without (P = 0.016 and P = 0.025, respectively). Furthermore, Kaplan-Meier analysis showed that cervical cancer patients with low miR-145 expression had shorter overall survival time than those with high miR-145 expression (P < 0.001). When analyzed with a multivariate Cox regression model, miR-145 was identified as an independent prognostic factor for overall survival. Taken together, our results suggest that downregulation of miR-145 in cervical cancer is associated with aggressive progression and poor prognosis and that miR-145 may serve as a prognostic marker.

MiR‐145 inhibits human colorectal cancer cell migration and invasion via PAK4‐dependent pathway

“…Also, downregulation of miR‐145‐5p correlated with poor prognosis in gastric cancer . The same conclusion was also obtained in oral squamous cell carcinomas , cervical cancer , osteosarcoma , and prostate cancer .These results convinced us that lower expression of miR‐145 may predict poor prognosis. And a further study is needed to demonstrate this conclusion.…”

Cucurbitacin D exhibits potent anti-cancer activity in cervical cancer

“…These modulated miRNAs in cancer can function as oncogenes or tumor suppressor genes. Studies have reported that miR-145 and miR143 are significantly lower in cervical cancer and expression of these miRNA inhibits growth of cervical cancer cells3435. Our data show a significant (p < 0.01) increase of miR-145 and miR-143 in cervical cancer cells after treatment with Cucurbitacin D, which clearly indicates that Cucurbitacin D may inhibit the growth of cervical cancer cells via inducing the expression of tumor suppressing miR-145 and miR-143.…”

“…5Bi) compared to vehicle control. Studies have also demonstrated that expression of miR-145 and miR-143 are significantly lower in cervical cancer when compared with their normal counterparts and over-expression of these miRNAs inhibit growth of cervical cancer cells3435. Thus, we were interested to determine whether Cucurbitacin D treatment restores the expression of these tumor suppressor miRNAs in cervical cancer cells.…”

MicroRNA-based potential diagnostic, prognostic and therapeutic applications in triple-negative breast cancer

“…MicroRNAs(miRNAs), a family of 21-25 nucleotide small non-protein-coding RNAs, are significantly involved in a variety of pathophysiological processes such as cell migration, invasion, proliferation and differentiation [12,13]. MicroRNAs can silence target genes efficiently and regulate a broad set of genes of interest simultaneously, which are involved in the initiation, progression and survival of TNBC [14][15][16].…”