Downregulation of BLM RecQ helicase inhibits proliferation, promotes the apoptosis and enhances the sensitivity of bladder cancer cells to cisplatin

Abstract

Bloom syndrome protein (BlM) is known to maintain genomic integrity including dna repair, recombination, replication and transcription. its dysregulation affects the genomic instability of cells, which results in a high risk of developing various types of cancer and even Bloom syndrome. However, to date, to the best of our knowledge, no association has been made between human BlM and bladder cancer. Thus, the aim of the present study was to investigate the role of BlM in human bladder cancer. The expression pattern of BlM in bladder cancer tissue was detected by immunohistochemistry. The viability, proliferation, cell cycle and apoptosis of bladder cancer cell lines were determined by Cell Counting Kit-8, EdU and flow cytometry following transfection of BlM small interfering rna. Finally, the effect of BlM on sensitivity of bladder cancer cell lines to cisplatin was investigated by reverse transcription-quantitative Pcr and western blot. it was demonstrated that the expression of BlM in human bladder cancer was increased compared with adjacent healthy bladder tissues. in addition, silencing of BlM inhibited the proliferation and promoted the apoptosis of bladder cancer cells and it also enhanced the sensitivity of bladder cancer cells to cisplatin. Together, the findings of the present study demonstrated that the regulation of BlM activity may have potential for use as a novel therapeutic target and a predictor for the prognosis of bladder cancer.

Bloom syndrome patients and mice display accelerated epigenetic aging

“…This may be explained by the decreased proliferative capacity of postnatal mouse brain resembling cellular features from Bloom mice (Semenov, 2021 ). Indeed, cells from BSyn patients also display decreased DNA replication (Subramanian et al, 2021 ) and silencing of BLM in vitro leads to inhibition of viability and proliferation (Feng et al, 2022 ). This was a surprising result that merits further investigation.…”

The Expression Characteristics and Function of the RECQ Family in Pan-Cancer

“…On the other hand, ER-positive breast cancer patients who had a RECQL protein level above the 75th percentile experienced improved DSS over a 15-year period, particularly among women [ 60 ]. Increased expression of BLM is associated with decreased OS in bladder cancer and reduced DFI in cholangiocarcinoma [ 13 , 61 ]. Breast cancer patients with high RECQL5 expression have a negative prognosis [ 62 ].…”